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We narrowed to 2,224 results for: Pam

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Showing: 601 - 620 of 2224 results

  1. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65767-plasmid-map-sequence-id-471631

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  3. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

    Plasmid
    #160140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Insert
    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    160140-plasmid-map-sequence-id-317331

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  5. dxCas(3.7)-VPR

    Plasmid
    #108383
    Purpose
    Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus
    Depositor
    David Liu
    Article
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Insert
    dxCas(3.7)-VPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Available Since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    108383-plasmid-map-sequence-id-207172

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  7. pNA0304

    Plasmid
    #165612
    Purpose
    Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    NotI site replaced the CAN1 spacer in parent vect…
    Promoter
    SNR52
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165612-plasmid-map-sequence-id-331070

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  9. MSP469

    Plasmid
    #65771
    Purpose
    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65771-plasmid-map-sequence-id-112653

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  10. BPK2101

    Plasmid
    #65770
    Purpose
    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    Use
    CRISPR
    Tags
    NLS-3xFlag
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65770-plasmid-map-sequence-id-112650

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  11. MSP680

    Plasmid
    #65772
    Purpose
    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65772-plasmid-map-sequence-id-112654

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  12. pEND-164_Cacnes_pFAST

    Plasmid
    #225616
    Purpose
    Replicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    pFAST
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225616-plasmid-map-sequence-id-451365

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  14. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

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  15. pEND-224_Cacnes_sfGFP

    Plasmid
    #225611
    Purpose
    Replicative vector for green fluorescent reporter protein sfGFP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+sfGFP.
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    sfGFP
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225611-plasmid-map-sequence-id-451364

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  16. dxCas(3.6)-VPR

    Plasmid
    #108388
    Purpose
    Mammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminus
    Depositor
    David Liu
    Article
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Insert
    dxCas(3.6)-VPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …
    Available Since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    108388-plasmid-map-sequence-id-207184

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  17. pEND-229_Cacnes_EforCP

    Plasmid
    #225615
    Purpose
    Replicative vector for red fluorescent reporter protein eForCP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+eForCP
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    EforCP
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225615-plasmid-map-sequence-id-451383

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  18. pEND-303_Cacnes_tetR

    Plasmid
    #225618
    Purpose
    C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherry
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    TetR
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225618-plasmid-map-sequence-id-451367

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  19. pEND-304_Cacnes_tetR

    Plasmid
    #225619
    Purpose
    C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO1O3)+RBS_1+mCherry
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    TetR
    Use
    Synthetic Biology
    Tags
    6xHis and mCherry-V5-6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225619-plasmid-map-sequence-id-451400

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  20. pEND-375_Cacnes_phlF

    Plasmid
    #225620
    Purpose
    C. acnes with PhlF-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+PhlF // P(BBa_23119-PhlO1O3)+RBS_1+mCherry.
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    PhlF
    Use
    Synthetic Biology
    Tags
    6xHis and mCherry-V5-6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225620-plasmid-map-sequence-id-451415

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Showing: 601 - 620 of 2224 results