We narrowed to 16,162 results for: grna

1. M-ST1-sgRNA

   Plasmid

   #48672

   Purpose

   Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter
   

   Use

   CRISPR

   Promoter

   hU

   Available Since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATG9A sgRNA

   Plasmid

   #207557

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the ATG9A locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   CACTGAATACCAGCGCCTAG
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Chr1q_Cassette-Integration_gRNA

   Plasmid

   #195126

   Purpose

   gRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integration

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Chr1q gRNA
   

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. ATP1A1_G4_Q118R_Std_BFP-to-EGFP_Dual_epegRNA_tevopreQ1

   Plasmid

   #187459

   Purpose

   Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G4 Q118R pegRNA + EBFP to EGFP epegRNA (tevopreq1) (ATP1A1 Human)
   

   Use

   CRISPR; Prime editing

   Expression

   Mammalian

   Promoter

   Tandem U6 promoters

   Available Since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1_Cas9-hGem_gRNA2

   Plasmid

   #217659

   Purpose

   expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   sgRNA2 targeting the human AAVS1 safe harbour locus
   

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX459-sgRNA048_Grb10

   Plasmid

   #232934

   Purpose

   Expression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   Cas9-T2A-PuroR (Grb10 S. pyogenes)
   

   Expression

   Mammalian

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. WIPI2 sgRNA

   Plasmid

   #207554

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the WIPI2 locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   CGCGCGCCCAGCCATGAACC
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA

   Plasmid

   #110624

   Purpose

   A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   sgRNA for St1Cas9
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   LP1B and hU6

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgRNA with U6 promoter

   Plasmid

   #48962

   Purpose

   to drive the sgRNA expression under a U6 promoter

   Depositor

   Mario de Bono

   Article

   Chen et al Nucleic Acids Res. 2013 Sep 5.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiGuidPuro-hTP73_sgRNA-1

    Plasmid

    #88855

    Purpose

    CRISPR KO of Trp73

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp73
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pH-PABE-7-sgRNA

    Plasmid

    #115621

    Purpose

    Targeted A to G in rice

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    wtTadA-TadA7.10-nCas9-3*NLS
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p426_Cas9_gRNA-ARS208a

    Plasmid

    #87383

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS208a sequence GTCCGCTAAACAAAAGATCT in yeast chromosome 2.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS208a
    

    Use

    CRISPR

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. U6-sgRNAs-Crym

    Plasmid

    #200067

    Purpose

    For the knock down of Crym with an astrocyte specific mCherry expression

    Depositor

    Baljit Khakh

    Article

    Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

    Insert

    U6-3xsgRNA-Crym
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    GfaABC1D-mCherry

    Promoter

    U6, GfaABC1D

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. 4 gRNA concatemer

    Plasmid

    #84881

    Purpose

    Empty concatmer vector in which 4 gRNAs can be inserted

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. AM77_pU6.Sa-gRNA.CLYBL

    Plasmid

    #199237

    Purpose

    Expression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    S. aureus gRNA spacer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    RNA polymerase III promoter for human U6 snRNA

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPEPX-P3-sgRNAluc

    Plasmid

    #85590

    Purpose

    Integrate plasmid of Streptococcus pneumoniae, which can integrate sgRNA targeting luc gene, which encode luciferase, into the locus between amiF and treR. This vector can be used as the template for

    Depositor

    Jan-Willem Veening

    Article

    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

    Insert

    sgRNA targeting firefly luciferase encoding gene
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    P3

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pgRNA-R-gfp

    Plasmid

    #220927

    Purpose

    Expresses gfp using J23119 promoter and pgRNA_AT backbone

    Depositor

    Andrea Puhar

    Article

    Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

    Insert

    superfolder GFP
    

    Tags

    FLAG

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBSV2_Psyn-sgRNA500

    Plasmid

    #149613

    Purpose

    gRNA expression in B. burgdorferi, parental vector

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Psyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBbdCas9S_P0526-sgRNAmreB

    Plasmid

    #149654

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30, P0526

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. 2 gRNA concatmer

    Plasmid

    #84879

    Purpose

    Empty concatmer vector in which 2 gRNAs can be inserted

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only