We narrowed to 1,316 results for: sumo

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pSA1

Plasmid

#52261

Purpose

Bacterial expression of human 6His-tagged SUMO1 and SUMO-E1

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-1 (SUMO1 Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-NTID194-GSQ-PMLiva3MAS-P2A-BLAST

Plasmid

#208039

Purpose

Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; 3MAS mutant; K>R sumoylation sites, mutated SIM); selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

PML (isoform IVa; 3MAS) (PML Human)

Use

Lentiviral

Tags

FLAG, N-terminal Split-TurboID

Mutation

3MAS mutant: K65R, K160R and K490R; mutated SIM

Promoter

EFS

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-SENP1 (catalytic domain)

Plasmid

#16356

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP1 catalytic domain (SENP1 Human)

Tags

His

Expression

Bacterial

Mutation

Amino Acid 419 to 644 catalytic domain of human S…

Available Since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pET28a-SENP2 (catalytic domain)

Plasmid

#16357

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP2 catalytic domain (SENP2 Human)

Tags

His

Expression

Bacterial

Mutation

Amino Acid 365 to 590 catalytic domain of human S…

Available Since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pSC-PreE2

Plasmid

#52264

Purpose

Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linker

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

Ubc9 GST fusion (UBE2I Human)

Tags

GST

Expression

Bacterial

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pSC-IntE2

Plasmid

#52265

Purpose

Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linker

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

Ubc9 GST fusion (UBE2I Human, Synthetic)

Tags

GST and GyrA-intein

Expression

Bacterial

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-SENP8 (full length))

Plasmid

#16361

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP8 full length (SENP8 Human)

Tags

His

Expression

Bacterial

Mutation

human SENP8 full length, see Depositor Comments

Available Since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pThuAsol

Plasmid

#171675

Purpose

pThuAsol expression in E. coli

Depositor

Wilfred van der Donk

Article

Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

Insert

SUMO-TEV-ThuAsol

Tags

His-tag and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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MERS-CoV Mpro strain HCoV-EMC 3CL protease domain

Plasmid

#228646

Purpose

Bacterial expression of the MERS-CoV Mpro domain for biochemical assay.

Depositor

ASAP Discovery, Frank von Delft

Insert

MERS-Cov Mpro domain

Tags

His-SUMO

Expression

Bacterial

Available Since

March 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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CTA2-mNG211

Plasmid

#141167

Purpose

Expresses GST-SUMO tagged CTA2 Subunit with 11th Strand

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

SUMO-CTA2-mNG211

Tags

SUMO

Expression

Bacterial

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GATA1 K137R

Plasmid

#85694

Purpose

Mammalian expression of mouse GATA1 with K137R mutation

Depositor

Licio Collavin, Giannino Del Sal

Article

Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

Insert

GATA1 (Gata1 Mouse)

Expression

Mammalian

Mutation

K137R (SUMO-1 attachment site)

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
6xHis-TEV-SMO-1(GG)

Plasmid

#87054

Purpose

Expresses 6xHis-tagged smo-1(GG), with a TEV protease cleavage site between the tag and the ORF

Depositor

Ronald Hay

Article

Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

Insert

smo-1 (smo-1 Nematode)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

The last aa was removed to render processed SMO-1…

Promoter

T7

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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TRIPZ-CTID195-GSQ-UBnc-PURO

Plasmid

#208037

Purpose

Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

Ubnc (UBC Human)

Use

Lentiviral

Tags

Myc, C-terminal Split-TurboID

Mutation

L73P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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5H9M (SIAH2)

Plasmid

#79521

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

SIAH2 (SIAH2 Human)

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Promoter

T7-lacO

Available Since

June 15, 2016

Availability

Industry, Academic Institutions, and Nonprofits
Human Coxsackievirus A16 strain G10 inactive 2A protease

Plasmid

#228665

Purpose

Bacterial expression of the A71 2A protease, innactive and can be used as a control in assays

Depositor

ASAP Discovery, Frank von Delft

Insert

Enterovirus A71 2A protease

Tags

His-SUMO

Expression

Bacterial

Mutation

Avitag insertion and C2A mutations / Active site …

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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WNV isolate WN NY 2000-crow3356 NS5 RdRp full length

Plasmid

#228662

Purpose

Bacterial expression of the West Nile virus NS5 RdRp protein for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

West nile NS5 RdRp

Tags

His-SUMO

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-SENP6 (catalytic domain)

Plasmid

#16359

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP6 catalytic domain (SENP6 Human)

Tags

His

Expression

Bacterial

Mutation

Amino Acid 628 to 1112 catalytic domain of human …

Available Since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only
pET28-His6-Ubc9-K14R

Plasmid

#185759

Purpose

Bacterial expression of His6-Ubc9-K14R

Depositor

Jeffrey Bode

Article

Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

K14R

Promoter

T7

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-TEV-GEI-17(133-509)

Plasmid

#87056

Purpose

Expresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORF

Depositor

Ronald Hay

Article

Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

Insert

GEI-17 isfoomr f (aa 133-509) (gei-17 Nematode)

Tags

6xHis-TEV and GAGGGS linker

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1

Plasmid

#32022

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1

Tags

His6-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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