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bMS.346

Bacterial Strain

#220588

Purpose

Increase editing efficiency of retron and oligo recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Bacterial Resistance

None

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only
C321

Bacterial Strain

#48999

Purpose

Recoded E. coli MG1655 strain with wt UAG termination function (RF1 is still present)

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Ampicillin

Species

Escherichia coli

Available Since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
AiP13905 - pAAV-AiE0441h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3905)

Plasmid

#199774

Purpose

enhancer AAV for expression of SYFP2 in all striatal medium spiny neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

SYFP2

Use

AAV

Mutation

wtGFP plus mutations F46L F64L S65G S72A M153T V1…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12555 - pAAV-3xSP10ins-AiE0367h_3xC2-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2555)

Plasmid

#191722

Purpose

AI plasmid ALIAS: AiP12555. Expression of SYFP2 (yellow fluorescent protein) in striatal medium spiny neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP15571: pAAV-AiE1426m-minBG-iCre(R297T)-BGHpA (Alias: CN5571)

Plasmid

#230877

Purpose

AiE1426m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Sst-Chodl interneurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV2

Insert

iCre(R297T)

Use

AAV

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15481: pAAV-AiE0140h_3xC2-minBG-iCre(R297T)-4X2C-BGHpA (Alias: CN5481)

Plasmid

#230876

Purpose

AiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb interneurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

iCre(R297T)

Use

AAV

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12982 - pAAV-AiE0452h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2982)

Plasmid

#191709

Purpose

AI plasmid ALIAS: AiP12982. Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781)

Plasmid

#199777

Purpose

enhancer AAV for Cre recombinase expression in striatal indirect pathway D2-MSNs

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

iCre(R297T)

Use

AAV

Mutation

R297T for reduced recombinase efficiency.

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779)

Plasmid

#199776

Purpose

enhancer AAV for Cre recombinase expression in striatal direct pathway D1-MSNs

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

iCre(R297T)

Use

AAV

Mutation

R297T for reduced recombinase efficiency.

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12609 - pAAV-AiE0779m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2609)

Plasmid

#191705

Purpose

AI plasmid ALIAS: AiP12609. Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP15043 - pAAV-AiE1419m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN5043)

Plasmid

#224189

Purpose

AiE1419m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+/Pthlh+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15578 - pAAV-AiE0780m-minBG-iCre(R297T)-BGHpA (Alias: CN5578)

Plasmid

#224200

Purpose

AiE0780m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D1 MSNs

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

iCre(R297T)

Use

AAV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12610 - pAAV-AiE0780m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2610)

Plasmid

#191737

Purpose

AI plasmid ALIAS: AiP12610. Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

SYFP2

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP15457 - pAAV-AiE0441h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5457)

Plasmid

#224198

Purpose

AiE0441h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal MSNs

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

CoChR-EGFP

Use

AAV

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP13278: pAAV-AiE0779m_3xC2-minBG-ChR2(CRC)-EYFP-WPRE3-BGHpA (Alias: CN3278)

Plasmid

#214843

Purpose

Expression of ChR2(L132C, H134R, T159C)-EYFP in striatal direct pathway medium spiny neurons (D1 MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

ChR2(L132C, H134R, T159C)-EYFP

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14035: pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035)

Plasmid

#214853

Purpose

Expression of CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

CoChR-EGFP

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12689 - pAAV-AiE0682h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2689)

Plasmid

#191727

Purpose

AI plasmid ALIAS: AiP12689. Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP13038 - pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3038)

Plasmid

#191720

Purpose

AI plasmid ALIAS: AiP13038. Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

SYFP2

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496)

Plasmid

#214858

Purpose

Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

SYFP2

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP13780: pAAV-AiE0743m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3780)

Plasmid

#214848

Purpose

Expression of iCre(R297T) in striatal cholinergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV2

Insert

iCre(R297T)

Use

AAV

Mutation

R297T

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge