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pEGFP VAMP7 (1-120)

Plasmid

#42317

Depositor

Thierry Galli

Article

Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

Insert

VAMP7 Longin domain (1-120) (VAMP7 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Δ120-220

Promoter

CMV

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only
pD649-HAsp-TNFRSF17-COMP5AP-AviTag-9xHis

Plasmid

#157053

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TNFRSF17 (TNFRSF17 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 BA.5

Plasmid

#212505

Purpose

Encodes SARS-CoV-2 variant BA.5 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BA.5 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIR2DS3-COMP5AP-AviTag-9xHis

Plasmid

#157237

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DS3 (KIR2DS3 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-KIR2DS5-COMP5AP-AviTag-9xHis

Plasmid

#157238

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DS5 (KIR2DS5 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-KIR2DP1-COMP5AP-AviTag-9xHis

Plasmid

#157227

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DP1 (KIR2DP1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 ORF3B

Plasmid

#141272

Purpose

Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF3B

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pD649-HAsp-PTPRS-COMP5AP-AviTag-9xHis

Plasmid

#157602

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PTPRS (PTPRS Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-SEMA5A-COMP5AP-AviTag-9xHis

Plasmid

#157572

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA5A (SEMA5A Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP VAMP7 (102-220)

Plasmid

#42314

Depositor

Thierry Galli

Article

Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

Insert

VAMP7 Coiled Coil domain (102-220) (VAMP7 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Δ1-102

Promoter

CMV

Available Since

March 13, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV-IRES-mOrange/CALR del52

Plasmid

#214768

Purpose

Mammalian expression of human CALR del52

Depositor

Norio Komatsu

Article

De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

Insert

human CALR del52 (CALR Human)

Use

Retroviral

Promoter

MSCV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-TNFSF12-COMP5AP-AviTag-9xHis

Plasmid

#157187

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TNFSF12 (TNFSF12 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-PILRA-COMP5AP-AviTag-9xHis

Plasmid

#157142

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PILRA (PILRA Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTT3-SP-6xHis-NTRK1(FL)-FLAG

Plasmid

#157620

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NTRK1 (NTRK1 Human)

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pInducer20 ALPK1 V1092A

Plasmid

#231588

Purpose

ALPK1 gene mutated in position V1092A (mutation found in spiradenomacarcinoma (sweat gland cancer)) under control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Article

Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

Insert

ALPK1 (ALPK1 Human)

Use

Lentiviral

Tags

3X flag

Expression

Mammalian

Mutation

changed Valine1092 to Alanine

Promoter

doxycycline

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CEACAM5-COMP5AP-AviTag-9xHis

Plasmid

#157501

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CEACAM5 (CEACAM5 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTT3-SP-6xHis-KIR2DL1(FL)-FLAG

Plasmid

#157625

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL1 (KIR2DL1 Human)

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTT3-SP-6xHis-KIR2DL4(FL)-FLAG

Plasmid

#157624

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL4 (KIR2DL4 Human)

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 LB.1

Plasmid

#223277

Purpose

Encodes SARS-CoV-2 spike LB.1 for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike LB.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-CEACAM6-COMP5AP-AviTag-9xHis

Plasmid

#157287

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CEACAM6 (CEACAM6 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge