164,892 results

1. pFastBac1-Nucleocapsid

   Plasmid

   #179696

   Purpose

   Expresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cells

   Depositor

   Mark Arbing

   Insert

   SARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   His6-TEV protease site

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Jan. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF1a-nSpCas9

   Plasmid

   #171691

   Purpose

   Expresses nSpCas9 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   nSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   EF1a

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pM162

   Plasmid

   #173221

   Purpose

   Expresses ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP(1-9)-GB1 and Puro-T2A-MCP-mCherry-GFP11-GB1

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Inserts

   ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP (1-10)-GB1
   MCP-mCherry-GFP11-GB1
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-mCherry-Tandem-PSAM4-GlyR

   Plasmid

   #208361

   Purpose

   Expression of the inhibitory chemogenetic tool PSAM4-GlyR and mCherry reporter. Application of ultrapotent agonists induces neuronal silencing.

   Depositor

   David Bennett

   Article

   Perez-Sanchez et al Sci Transl Med. 2023 Oct 4;15(716):eadh3

   Insert

   mCherry-TANDEM-PSAM4-GlyR
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3 MKK7b1(K149A)

   Plasmid

   #14674

   Depositor

   Roger Davis

   Article

   Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

   Insert

   MKK7b1(K149A) (Map2k7 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Lys-149Ala

   Promoter

   Available since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

6. GFP-WIPI2d-TEVcs-TSF

   Plasmid

   #171420

   Purpose

   For the purifcation of GFP- WIPI2d protein

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. eNS2B47NS3 L30SF31S

   Plasmid

   #155319

   Purpose

   DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker L30SF31S mutant

   Depositor

   Dahai Luo

   Article

   Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

   Insert

   DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   L30 to S, F31 to S

   Promoter

   M13

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYJA5

   Plasmid

   #217778

   Purpose

   The empty vector for quadruple sgRNA cloning

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Puromycin resistance gene, T2A, and TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   PGK, CMV, U6

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEBTet-SNAP-POC5

   Plasmid

   #136786

   Purpose

   Mammalian expression of the centrosomal protein POC5 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-POC5 (POC5 Human, Synthetic)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA6.2 EmGFP hAQP4(M23)

    Plasmid

    #126464

    Purpose

    Expresses human AQP4 (isoform M23) as an EmGFP fusion protein in mammalian cells

    Depositor

    Markus Reindl

    Article

    Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

    Insert

    Aquaporin 4 (AQP4 Human)
    

    Use

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. GtCCR2_EYFP_pcDNA3.1

    Plasmid

    #159121

    Purpose

    This plasmid encodes GtCCR2 rhodopsin domain

    Depositor

    John Spudich

    Article

    Sineshchekov et al Proc Natl Acad Sci U S A. 2017 Nov 7;114

    Insert

    GtCCR2
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSPneogRNA241510+MT

    Plasmid

    #84292

    Purpose

    Express LdPBK_241510.1 and LdMT targeting gRNAs simutaneously

    Depositor

    Greg Matlashewski

    Article

    Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

    Insert

    LdBPK_241510.1 targeting gRNA and LdMT targeting gRNA
    

    Use

    CRISPR; Leishmania donovani

    Tags

    Expression

    Mutation

    Promoter

    L. donovani ribosome RNA promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-RILP

    Plasmid

    #110498

    Purpose

    Fluorescent RILP

    Depositor

    Terence Dermody, Bernardo Mainou

    Article

    Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

    Insert

    RILP (RILP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEBTet-SNAP-STIL

    Plasmid

    #136795

    Purpose

    Mammalian expression of the centrosomal protein STIL N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-STIL (STIL Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX552-mScn2a-3xV5-EF1-smFP-flag

    Plasmid

    #182561

    Purpose

    For mouse Nav1.2 knockin with 3xV5 at the C-terminal

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    mouse Scn2a gRNA and 3xV5 donor DNA
    smFP-flag
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1 and U6

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHybr_r2a>mA-srt-his

    Plasmid

    #124806

    Purpose

    HDR template to knock-in mouse IgA Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgA producing cell lines.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Murine IgA
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LCV2_AAVS1_sgRNA_2

    Plasmid

    #155088

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNG6

    Plasmid

    #30990

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    repeat NG6
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (AAV9)

    Viral Prep

    #162382-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (#162382). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of the ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    CAG

    Tags

    None

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

20. pRECMYC-Ebu1

    Plasmid

    #240713

    Purpose

    pLAM12 vector backbone containing Ebu1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

    Depositor

    Seth Shipman

    Insert

    Ebu1 RT, Eco1 ncRNA, MspRecT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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