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pMLM3705

Plasmid

#47754

Purpose

Expresses mammalian cell codon-optimized dCas9-VP64

Depositor

Keith Joung

Publication

Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

codon optimized dCas9-VP64

Use

CRISPR

Tags

3x FLAG, NLS, and VP64

Expression

Mammalian

Mutation

D10A and H840A mutations in Cas9 (catalytically i…

Promoter

CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pCAG-T7-TALEN(Sangamo)-Destination

Plasmid

#37184

Purpose

Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.

Depositor

Pawel Pelczar

Publication

Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

Type

Empty backbone

Tags

FokI nuclease

Expression

Mammalian

Promoter

CAG

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAG-T7-TALEN(Sangamo)-Destination

Plasmid

#37184

Purpose

Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.

Depositor

Pawel Pelczar

Publication

Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

Type

Empty backbone

Tags

FokI nuclease

Expression

Mammalian

Promoter

CAG

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-Gateway

Plasmid

#32671

Depositor

Matthew Nolan

Publication

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Type

Empty backbone

Use

AAV; Aav

Available since

June 29, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-Gateway

Plasmid

#32671

Depositor

Matthew Nolan

Publication

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Type

Empty backbone

Use

AAV; Aav

Available since

June 29, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-Ptet-RFP-shR-rtTA

Plasmid

#35625

Purpose

Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

Depositor

Howard Gu

Publication

Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

Type

Empty backbone

Use

AAV and RNAi; Tet inducible

Expression

Mammalian

Promoter

Ptet

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Ptet-RFP-shR-rtTA

Plasmid

#35625

Purpose

Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

Depositor

Howard Gu

Publication

Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

Type

Empty backbone

Use

AAV and RNAi; Tet inducible

Expression

Mammalian

Promoter

Ptet

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FRB-NLuc

Plasmid

#31181

Depositor

David Piwnica-Worms

Publication

Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rapamycin-binding domain and the N-terminus of Firefly Luciferase (MTOR Human)

Expression

Mammalian

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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FRB-NLuc

Plasmid

#31181

Depositor

David Piwnica-Worms

Publication

Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rapamycin-binding domain and the N-terminus of Firefly Luciferase (MTOR Human)

Expression

Mammalian

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCDNA3.1-Nanog

Plasmid

#28221

Depositor

Linzhao Cheng

Publication

Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nanog (NANOG Human)

Expression

Mammalian

Promoter

CMV

Available since

March 2, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCDNA3.1-Nanog

Plasmid

#28221

Depositor

Linzhao Cheng

Publication

Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nanog (NANOG Human)

Expression

Mammalian

Promoter

CMV

Available since

March 2, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pMLM800

Plasmid

#27202

Depositor

Keith Joung

Publication

Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

Insert

FokI endonuclease

Use

Zebrafish expression

Tags

FLAG

Expression

Mammalian

Available since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMLM802

Plasmid

#27203

Depositor

Keith Joung

Publication

Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

Insert

FokI endonuclease

Use

Zebrafish expression

Tags

FLAG

Expression

Mammalian

Available since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pMLM800

Plasmid

#27202

Depositor

Keith Joung

Publication

Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

Insert

FokI endonuclease

Use

Zebrafish expression

Tags

FLAG

Expression

Mammalian

Available since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pMLM802

Plasmid

#27203

Depositor

Keith Joung

Publication

Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

Insert

FokI endonuclease

Use

Zebrafish expression

Tags

FLAG

Expression

Mammalian

Available since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pAC-GAL4

Plasmid

#24344

Depositor

Liqun Luo

Publication

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

GAL4

Expression

Insect

Available since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pAC-GAL4

Plasmid

#24344

Depositor

Liqun Luo

Publication

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

GAL4

Expression

Insect

Available since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

Enlarge
VCP(R155H)-EGFP

Plasmid

#23972

Depositor

Nico Dantuma

Publication

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Valosin containing protein (VCP Human)

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

R155H

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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VCP(R155H)-EGFP

Plasmid

#23972

Depositor

Nico Dantuma

Publication

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Valosin containing protein (VCP Human)

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

R155H

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pCMV-Caspase1-flag

Plasmid

#21142

Depositor

Junying Yuan

Publication

Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

caspase-1 (Casp1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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