We narrowed to 10,563 results for: ESP

1. pMOD_C2516b

   Plasmid

   #91085

   Purpose

   Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, (+ BaeI site for GT donor cloning), Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Promoter

   At7SL

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-WRAP53beta-DC-mutant-G435R

   Plasmid

   #64684

   Purpose

   expresses single mutated WRAP53beta at G435R, found in Dyskeratosis congenita patients

   Depositor

   Marianne Farnebo

   Article

   Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

   Insert

   WD40-encoding RNA antisense to P53 (WRAP53 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   mutation at G435R

   Promoter

   CMV

   Available Since

   May 21, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pEGFP-WRAP53beta-DC-mutant-R398W

   Plasmid

   #64683

   Purpose

   expresses single mutated WRAP53beta at R398W, found in Dyskeratosis congenita patients

   Depositor

   Marianne Farnebo

   Article

   Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

   Insert

   WD40-encoding RNA antisense to P53 (WRAP53 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   mutation at R398W

   Promoter

   CMV

   Available Since

   May 21, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pEE038

   Plasmid

   #176795

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   407
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEE048

   Plasmid

   #176805

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   504
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST27-GST-ATXN1 FL(2Q)

   Plasmid

   #21752

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains 2Q (2 glutamines) and there isno …

   Promoter

   CMV

   Available Since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3 Flag MFF iso1 WT

   Plasmid

   #74388

   Purpose

   Flag tagged human MFF isoform 1

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   delta exon 1 (1-26)

   Available Since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pGL2 p15 4xSBR2 Mut 3' box

   Plasmid

   #15708

   Depositor

   Joan Massague

   Article

   Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

   Insert

   p15 4xSBR2 (CDKN2B Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutant 3' box. The 3' box contains the …

   Available Since

   Sept. 7, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3 Flag MFF iso4 WT

   Plasmid

   #74390

   Purpose

   Flag tagged human MFF isoform 4

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pEGFP-WRAP53beta-DC-mutant-F164L

    Plasmid

    #64681

    Purpose

    expresses single mutated WRAP53beta at F164L, found in Dyskeratosis congenita patients

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    WD40-encoding RNA antisense to P53 (WRAP53 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    mutation at F164L

    Promoter

    CMV

    Available Since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pEGFP-WRAP53beta-DC-mutant-H376Y

    Plasmid

    #64682

    Purpose

    expresses single mutated WRAP53beta at H376Y, found in Dyskeratosis congenita patients

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    WD40-encoding RNA antisense to P53 (WRAP53 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    mutation at H376Y

    Promoter

    CMV

    Available Since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA-p160(400-580)

    Plasmid

    #45

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    expresses only aa400-580

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA-p160(200-400)

    Plasmid

    #44

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Expresses only aa200-400

    Available Since

    July 21, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA5 FRT TO myc Separase S1226A/ C2029A

    Plasmid

    #59823

    Purpose

    Allows the integration of myc Separase S1226A/ C2029A in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

    Insert

    Separase (ESPL1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S1226A/ C2029A

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDual-eGFP(Stop66)

    Plasmid

    #63218

    Purpose

    Expression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP(Stop66)
    

    Use

    Lentiviral

    Tags

    T7 and his6

    Expression

    Bacterial and Mammalian

    Mutation

    Changed tyrosine at position 66 with respect to t…

    Promoter

    CMV-EF1α hybrid (CEF)

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCin4-3xFLAG-MKL1 D444-500

    Plasmid

    #19839

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 D444-500 (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 444-500

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pGL3b(1454/3172)P2P-wt

    Plasmid

    #21404

    Depositor

    Eric Metzen

    Article

    Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

    Insert

    EGLN1 (EGLN1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pCin4-3xFLAG-MKL1 T450A

    Plasmid

    #19843

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 T450A (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Threonine 450 to Alanine

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pDual-eGFP(W66)

    Plasmid

    #63217

    Purpose

    Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP(W66)
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Changed tyrosine at position 66 with respect to t…

    Promoter

    CMV-EF1α hybrid (CEF)

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA5FRT/TO-UBXD5-Strep-HA

    Plasmid

    #113493

    Purpose

    Expression of human UBXD5 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    UBXD5 (UBXN11 Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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