We narrowed to 15,981 results for: LEA

1. SuExp-hPLD3

   Plasmid

   #201243

   Purpose

   express full length human PLD3 protein

   Depositor

   David Nemazee

   Article

   Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

   Insert

   PLD3 (PLD3 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.10 Dub3

   Plasmid

   #72684

   Purpose

   luciferase reporter with Dub3 promoter

   Depositor

   Domenico Maiorano

   Article

   van der Laan et al PLoS One. 2014 Apr 2;9(4):e93663. doi: 1

   Insert

   Dub3 promoter (Usp17le Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Dub3

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHLmMBP-10

   Plasmid

   #72348

   Purpose

   Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Tags

   6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

   Expression

   Mammalian

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpCas9-NG-YE1-BE4max

   Plasmid

   #198535

   Purpose

   Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-YE1-BE4max
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLIB-WDR61

   Plasmid

   #231729

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Insert

   WDR61 (SKIC8 Human)
   

   Expression

   Insect

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cas9-NAT

   Plasmid

   #64329

   Purpose

   Cas9 expression cassette carried by a yeast single-copy episome plasmid

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sec61 beta pAcGFP-C1 (802)

   Plasmid

   #62008

   Purpose

   mammalian expression of GFP-Sec61 beta

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   Sec61 beta (SEC61B Human)
   

   Tags

   AcGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-Ef1a-Coff/Fon-EYFP

   Plasmid

   #231927

   Purpose

   Cre-off/Flp-on EYFP under the Ef1a promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   eYFP with introns
   

   Use

   AAV

   Promoter

   Ef1a

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-DsRed_IRES_SNCA:EGFP

   Plasmid

   #92195

   Purpose

   Lentiviral overexpression vector to make stable bicistronic cell line for α-Syn screen

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   DsRed-IRES-SNCA-EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

10. AAV-CAG-SPOTlight-U2GCR

    Plasmid

    #164819

    Purpose

    Reporter to measure ISR state-dependent protein synthesis

    Depositor

    Nicole Calakos

    Article

    Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

    Insert

    SPOTlight-U2GCR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-EF1a-CCO-IRES-Puromycin

    Plasmid

    #134861

    Purpose

    Expresses EGFP-tagged inner mitochondrial membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    cytochrome c oxidase subunit VIII (COX8A Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGPTVII-nlsSED1

    Plasmid

    #178190

    Purpose

    SED1 biosensor for expression in Arabidopsis thaliana

    Depositor

    Jose Dinneny, Cesar Cuevas-Velazquez

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 biosensor
    

    Tags

    SV40 NLS

    Expression

    Plant

    Promoter

    pUBQ10

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-CD81-(TEV)-FKBP-SmBiT

    Plasmid

    #182651

    Purpose

    human CD81-TEV cleavage site-FKBP-SmBiT tag

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

    Insert

    human CD81-TEVp cleavage site-FKBP-SmBiT (CD81 Synthetic, Human)
    

    Tags

    SmBiT

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term

    Plasmid

    #182925

    Purpose

    Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Type

    Empty backbone

    Tags

    APEX2 and FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMH-SFB-DYRK1A

    Plasmid

    #101770

    Purpose

    Mammalian expression of DYRK1A fusion protein

    Depositor

    Michael Huen

    Article

    Li et al Nucleic Acids Res. 2024 Jul 5:gkae574. d

    Insert

    DYRK1A (DYRK1A Human)
    

    Tags

    S peptide-flag-streptavidin binding peptide (SFB)

    Expression

    Mammalian

    Mutation

    siRNA-resistant allele

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-term

    Plasmid

    #182926

    Purpose

    Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Type

    Empty backbone

    Tags

    APEX2 and FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pShuttle-Flag-XBP1u

    Plasmid

    #63679

    Purpose

    Adenoviral shuttle vector for XBP1u expression

    Depositor

    Lingfang Zeng, Qingbo Xu

    Article

    Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

    Insert

    XBP1 (XBP1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    unspliced

    Promoter

    CMV

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR

    Plasmid

    #230935

    Purpose

    gRNA cloning vector encoding BFP and puromycin resistance

    Depositor

    Charles Gersbach

    Article

    Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP

    Promoter

    hU6

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS

    Plasmid

    #187588

    Purpose

    Express FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    NLS
    

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETM11-6xHis-TEV-hORF1p

    Plasmid

    #202589

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Kathleen Burns, Liam Holt

    Article

    Taylor et al Cell. 2013 Nov 21;155(5):1034-48. doi: 1

    Insert

    ORF1p
    

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Available Since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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