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pFAB3906

Plasmid

#47809

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD2 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD2

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pINEAPLE

Plasmid

#250295

Purpose

Big-IN Payload assembly vector by Golden Gate Cloning using Esp3I. Contains a bacterial green/white GFP-based screening system and a mammalian transient (backbone) BSD selection cassette.

Depositor

Matthew Maurano

Article

Ordoñez et al bioRxiv 2025.08.13.669251

Insert

SuperfolderGFP

Use

Synthetic Biology; Assembly vector for big-in pay…

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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UbSYRAP_pDONR201

Plasmid

#249816

Purpose

Encodes UbSYRAP, a SYRAP split version (UBQ-RAP2.12-GAL4STE12), Gateway compatible as Donor plasmid

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Insert

UBQ-RAP2.12-GAL4STE (RAP2.12 Budding Yeast, Mustard Weed)

Use

Synthetic Biology

Tags

GAL4-STE12 and UBQ

Expression

Plant and Yeast

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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RPS6KA3-P1

Plasmid

#236315

Purpose

Study the role and mechanism of the 3' UTR in regulating RPS6KA3-P1 gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

RPS6KA3-P1 3'UTR (RPS6KA3 Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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RPS6KA3-P3

Plasmid

#236316

Purpose

Study the role and mechanism of the 3' UTR in regulating RPS6KA3-P3 gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

RPS6KA3-P3 3'UTR (RPS6KA3 Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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NCOA7-P1

Plasmid

#237834

Purpose

Study the role and mechanism of the NCOA7-P1 3' UTR in regulation gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

NCOA7-P1 3'UTR (NCOA7 Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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NCOA7-P2

Plasmid

#237835

Purpose

Study the role and mechanism of the NCOA7-P2 3' UTR in regulation gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

NCOA7-P2 3'UTR (NCOA7 Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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OPTN-P1

Plasmid

#237836

Purpose

Study the role and mechanism of the OPTN-P1 3' UTR in regulation gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

OPTN-P1 3'UTR (OPTN Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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OPTN-P2

Plasmid

#237837

Purpose

Study the role and mechanism of the OPTN-P2 3' UTR in regulation gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

OPTN-P2 3'UTR (OPTN Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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MQ1v-dCas9-BFP (M39)

Plasmid

#239485

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-MQ1v-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MQ1v-dCas9-cSRDX (M40)

Plasmid

#239486

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-MQ1v-XTEN80-NLS-dCas9-2xNLS-XTEN16-SRDX

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pVCS7

Plasmid

#231621

Purpose

Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS1 at R2 site.

Depositor

Hans Christopher Bernstein

Article

Chan et al ACS Synth Biol. 2025 Jan 17;14(1):193-20

Insert

Pcym-VanR-mKate2-Pvan-CymR-sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pvan/Pcym

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pmRNA-iEMCV-EGFP-A120

Plasmid

#225905

Purpose

In vitro transcription of cap-independently (EMCV IRES) translated mRNA encoding EGFP.

Depositor

Tom Brown

Article

Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

Inserts

A-inserted T7 class III Φ6.5 promoter
EMCV IRES
EGFP
2x β-globin UTR
poly(A) tail

Use

Synthetic Biology; Mrna preparation

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pmRNA-iHCV-EGFP-A120

Plasmid

#225906

Purpose

In vitro transcription of cap-independently (HCV IRES) translated mRNA encoding EGFP.

Depositor

Tom Brown

Article

Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

Inserts

A-inserted T7 class III Φ6.5 promoter
HCV IRES
EGFP
2x β-globin UTR
poly(A) tail

Use

Synthetic Biology; Mrna preparation

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCK761

Plasmid

#242916

Purpose

Constitutive expression of dCas9, αNTD-MCP, and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCK948

Plasmid

#242917

Purpose

Constitutive expression of dCas9, MCP-TetD, and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pKO025

Plasmid

#242913

Purpose

aTc-inducible expression of Spy-dCas9-AsiA and Sth1-dCas9, and constitutive expression of MCP-SoxS

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

Sth1-dCas9

Use

CRISPR and Synthetic Biology

Promoter

Ptet

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCK660

Plasmid

#242914

Purpose

Constitutive expression of dCas9, MCP-SoxS, and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCK320

Plasmid

#242906

Purpose

aTc-inducible expression of dCas9-AsiA

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

AsiA (asiA Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

TetR-Ptet

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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mE-mCherry2A

Plasmid

#236248

Purpose

Middle entry fluorescent mCherry reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately

Depositor

James Amatruda, Genevieve Kendall

Article

Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10

Insert

mCherry2A

Expression

Mammalian

Mutation

Added a viral2A sequence to mCherry with no stop …

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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