We narrowed to 9,277 results for: Pol;

1. Frt-hspB5

   Plasmid

   #63096

   Purpose

   Expression of HSPB5 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb5 (CRYAB Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX458 eSp(1.1) V3

   Plasmid

   #226963

   Purpose

   CBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

   Plasmid

   #216477

   Purpose

   lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   dsRed
   tagBFP
   

   Use

   Cre/Lox and Lentiviral

   Promoter

   EF1aL and UBC

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUb-Cas9-EGFP_cU6:6-sgRNA

   Plasmid

   #190597

   Purpose

   The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.

   Depositor

   Claudia Rueckert

   Article

   Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR

   Tags

   EGFP - separated by T2A

   Promoter

   Aedes aegypti polyubiquitin and Culex quinquefasc…

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. mouse b3 full length

   Plasmid

   #217814

   Purpose

   Full length mouse integrin b3 expression

   Depositor

   Timothy Springer

   Article

   Yuxin et al bioRxiv 2024.01.26.577394

   Insert

   ITGB3 (Itgb3 Mouse)
   

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTMiniTrex-mCherry-Shark1

   Plasmid

   #233143

   Purpose

   Serves as a template plasmid for amplifying Shark1 (Tet-OFF) hammerhead aptazyme-3xTy-tagging cassette

   Depositor

   Ronald Etheridge

   Article

   Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

   Inserts

   mCherry
   Shark1
   

   Use

   Prokaryotic expression

   Tags

   3x Ty tag

   Mutation

   Codon optimized for Trypanosoma cruzi and changed…

   Available Since

   March 25, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pET28_6xHis-PARP1delBRCT

   Plasmid

   #173938

   Purpose

   Bacterial expression of PARP1 lacking BRCT domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delBRCT (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 374-520; V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. hu-TLR4 cDNA wt pDEST40

   Plasmid

   #42646

   Purpose

   destination vector for expression of human TLR4 in mammalian cells

   Depositor

   Scott Friedman

   Article

   Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

   Insert

   TLR4 (TLR4 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pLentiDP1.3

   Plasmid

   #171096

   Purpose

   lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Cdt1 (AKA ROLECCS G1) and miniAID-fused mCherry2

   Depositor

   Dario Palmieri

   Article

   Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

   Insert

   OsTIR1-mEmerald-Cdt1-P2A-miniAID-mCherry2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Emerald, cdt1, miniAID, mCherry2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-MS2V5-eRF3a F76a

    Plasmid

    #65811

    Purpose

    expression of eRF3a-MS2 fusion. F76A mutation abolishes binding to PABPC1

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    eRF3a (GSPT1 Human)
    

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Mutation

    F76a

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Frt-V5-hspB5

    Plasmid

    #63106

    Purpose

    Expression of HSPB5 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb5 (CRYAB Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiDP1.2

    Plasmid

    #171095

    Purpose

    lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS G2) and miniAID-fused mCherry2

    Depositor

    Dario Palmieri

    Article

    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

    Insert

    OsTIR1-mEmerald-Geminin-P2A-miniAID-mCherry2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Emerald, Geminin, miniAID, mCherry2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Cdt1 human

    Plasmid

    #72681

    Purpose

    mammalian expression of human Cdt1

    Depositor

    Domenico Maiorano

    Article

    Tsanov et al Nucleic Acids Res. 2014 Apr;42(6):3692-7

    Insert

    Cdt1 (CDT1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

14. p2706-pHAGE-EF1aL-YAP5SA-UBC-GFP

    Plasmid

    #216475

    Purpose

    lentiviral dual expression of YAP5SA and eGFP

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    YAP5SA
    eGFP
    

    Use

    Lentiviral

    Mutation

    S61A, S109A, S127A, S164A, S381A

    Promoter

    EF1aL and UBC

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. LLP457 pGK-dCas9-Suntag-BFP

    Plasmid

    #100957

    Purpose

    BPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tag

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    dCas9, SunTag array (10xGCN4)
    

    Use

    CRISPR

    Tags

    3xHA and BFP

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac1-His10TEV

    Plasmid

    #159427

    Purpose

    empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

    Depositor

    Debra Hansen

    Article

    Olson et al Protein Expr Purif. 2021 May 7:105890. d

    Type

    Empty backbone

    Tags

    TEV-protease-cleavable His10-tag

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28_6xHis-PARP1delZn2

    Plasmid

    #173935

    Purpose

    Bacterial expression of PARP1 lacking Zn2 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn2 (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 97-213 (V762A)

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28_6xHis-PARP1-ART

    Plasmid

    #174810

    Purpose

    Bacterial expression of isolated PARP1 ART subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-ART (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28_6xHis-PARP1-BRCT

    Plasmid

    #174807

    Purpose

    Bacterial expression of isolated PARP1 BRCT domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-BRCT (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28_6xHis-PARP1-WGR

    Plasmid

    #174808

    Purpose

    Bacterial expression of isolated PARP1 WGR domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-WGR (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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