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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,323 results for: ina

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Showing: 6281 - 6300 of 25323 results

  2. CaMKIV-DCTK75E

    Plasmid
    #126423
    Purpose
    A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)
    Depositor
    Douglas Black
    Publication
    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    calcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed lysine 75 to glutamic acid
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126423-plasmid-map-sequence-id-249663

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  3. pcDNA3-MKK1K97A

    Plasmid
    #107974
    Purpose
    expression of a dominant negative MAP Kinase Kinase
    Depositor
    Edwin Krebs
    Publication
    Seger et al J Biol Chem. 1994 Oct 14;269(41):25699-7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MKK1 K97A (MAP2K1 Human)
    Expression
    Mammalian
    Mutation
    dominant negative; see Depositor Comments below
    Promoter
    CMV
    Available since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    107974-plasmid-map-sequence-id-213451

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  4. Alpha MHC-DN.JNK2

    Plasmid
    #51931
    Purpose
    mammalian expression of dominant negative JNK2
    Depositor
    Jeffery Molkentin
    Publication
    Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DN JNK2 (Mapk9 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    dominant negative: the dual phosphorylation moti…
    Promoter
    cardiac-specific α-myosin heavy chain 5.5 kb pro…
    Available since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

    Plasmid
    #173872
    Purpose
    Mammalian expression of mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)
    Depositor
    Takao Nakata
    Publication
    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
    Tags
    C-terminal CAAX-box and mCherry
    Expression
    Mammalian
    Available since
    Sept. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    173872-plasmid-map-sequence-id-341725

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  8. W45A mutant of H3K9me3 biosensor

    Plasmid
    #120808
    Purpose
    FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability
    Depositor
    Yingxiao Wang
    Publication
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
    Expression
    Mammalian
    Mutation
    Tryptophan 45 is mutated to Alanine 45 on HP1 dom…
    Promoter
    CMV
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120808-plasmid-map-sequence-id-235136

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  9. MSP2-bio

    Plasmid
    #47710
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP2 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon-optimised MSP2
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47710-plasmid-map-sequence-id-68424

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  18. pGBW-m4046747

    Plasmid
    #149249
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149249-plasmid-map-sequence-id-358332

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  19. pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS

    Plasmid
    #134653
    Purpose
    lysosome-targeted Sac1 catalytic domain C389S
    Depositor
    Roberto Zoncu
    Publication
    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218
    Insert
    Sac1 catalytic domain (77-520 aa) CS mutant (SACM1L Human)
    Use
    Lentiviral
    Tags
    FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…
    Expression
    Mammalian
    Mutation
    C389S and contains only amino acids 77-520 (Pleas…
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    134653-plasmid-map-sequence-id-266494

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  20. AICSDP-9:DSP-mEGFP

    Plasmid
    #87424
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human DSP
    Depositor
    Allen Institute for Cell Science
    Publication
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DSP Homology Arms with linker-mEGFP (DSP Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    87424-plasmid-map-sequence-id-173979

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Showing: 6281 - 6300 of 25323 results