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pXPR_BRD003 LIAS-1

Plasmid

#184478

Purpose

Lentivirus gRNA targeting human LIAS gene

Depositor

Peter Tsvetkov, Todd Golub

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

LIAS (LIAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 LIAS-2

Plasmid

#184479

Purpose

Lentivirus gRNA targeting human LIAS gene

Depositor

Peter Tsvetkov, Todd Golub

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

LIAS (LIAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CCND1(T286A)

Plasmid

#172631

Purpose

Expresses untagged cyclin D1(T286A) in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Tags

Expression

Mammalian

Mutation

Thr286Ala

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-eGFP-NLS-RNF168 delta RING

Plasmid

#133983

Purpose

mammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

deletion of RING; siRNA resistant sequence: GAGGA…

Promoter

CMV

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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W118-1_hRSK3(RPS6KA2)

Plasmid

#180384

Purpose

lentiviral transduction of human RSK3 (RPS6KA2) gene

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

RPS6KA2 (RPS6KA2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-PLXNB2-shRNA2

Plasmid

#98400

Purpose

Lentivirus for expression of shRNA2 against human PLEXIN-B2 (Dox-inducible)

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

Plexin-B2 (PLXNB2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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R620-M21-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.429

Plasmid

#170200

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.429 variant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) B.1.429 (S SARS-CoV-2)

Use

Tags

His6

Expression

Mammalian

Mutation

S13I, W152C, L452R, D614G

Promoter

CMV51

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

Plasmid

#136659

Purpose

Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD9:TH1477 chimera
sgRNA for St1Cas9
APOBEC-1 (APOBEC1 Human)
UGI
HA tag

Use

CRISPR and Synthetic Biology

Tags

2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

Expression

Mammalian

Mutation

Promoter

CAG and U6

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL WT

Plasmid

#107156

Purpose

MBP-rPKL WT for production and purification of rat PKL WT

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Use

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-hPKL S113D

Plasmid

#107161

Purpose

For mammalian expression of Flag-hPKL S113D

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

Flag-hPKL S113D (PKLR Human)

Use

Retroviral

Tags

N-term Flag

Expression

Mammalian

Mutation

Serine 113 mutated to aspartate

Promoter

CMV

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-hPKL S113A

Plasmid

#107160

Purpose

For mammalian expression of Flag-hPKL S113A

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

Flag-hPKL S113A (PKLR Human)

Use

Retroviral

Tags

N-term Flag

Expression

Mammalian

Mutation

Serine 113 mutated to alanine

Promoter

CMV

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pet-ECHB(35-475)

Plasmid

#96890

Purpose

expresses His-tagged mouse ECHB (35-475) in E.coli cells

Depositor

Hening Lin

Article

Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

Insert

Trifunctional enzyme subunit beta, mitochondrial (Hadhb Mouse)

Use

Tags

His6 tag

Expression

Bacterial

Mutation

deleted amino acid 1-34

Promoter

T7

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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LZF40: LZF40_Fsyn_Cre

Plasmid

#109378

Purpose

Synaptic transmission

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

Cre-NCBI

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human synapsin I

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJJR50

Plasmid

#75026

Purpose

U6 promoter driven flipped + extended sgRNA expression vector

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

guide RNA, flipped and extended version

Use

CRISPR

Tags

Expression

Mutation

Promoter

R07E5.16 (U6)

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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sgALK(Chr. Inv.)

Plasmid

#85569

Purpose

Expresses sgRNA targeting human ALK between exon 20 and 21

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

anaplastic lymphoma receptor tyrosine kinase (ALK Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pVZ_PcoaT_SQE1

Plasmid

#103860

Purpose

Heterologous, cobalt-inducible expression of SQE1 from A. thaliana in Synechocystis sp. PCC6803

Depositor

Ilka Axmann

Article

Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d

Insert

squalene epoxidase 1 (XF1 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

inserted gene is codon optimized. 2nd amino acid …

Promoter

PcoaT (from Synechocystis sp. PCC 6803)

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET-Duet-ECHA(37-763)-FLAG (no His-tag)

Plasmid

#96887

Purpose

expresses flag-tagged mouse ECHA (37-768) in E.coli cells (no His-tag)

Depositor

Hening Lin

Article

Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

Insert

Trifunctional enzyme subunit alpha, mitochondrial (Hadha Mouse)

Use

Tags

FLAG-tagged

Expression

Bacterial

Mutation

deleted amino acid 1-36

Promoter

T7

Available since

July 20, 2019

Availability

Academic Institutions and Nonprofits only

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sgEGFR(B)

Plasmid

#85565

Purpose

Expresses sgRNA targeting human EGFR around codon T790

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

epidermal growth factor receptor (EGFR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pCDF-casBCDE(+6)

Plasmid

#89728

Purpose

Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.

Depositor

Konstantin Kuznedelov, Konstantin Severinov

Article

Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

CRISPR array

Use

Tags

Expression

Bacterial

Mutation

Derivative CRISPR array containing a longer versi…

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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3' MBD3 TALEN

Plasmid

#52359

Purpose

TALEN vector for targeting human MBD3 locus (3')

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

NN NN HD NN NG NN NI HD HD HD HD NI NN NI HD HD NG

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only