We narrowed to 3,399 results for: guide rna expression plasmid

1. pCY5h

   Plasmid

   #160295

   Purpose

   Yeast CRISPR plasmid targeting the hphMX cassette

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
   URA3 selection cassette
   sgRNA expression cassette (without guide)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCY5n

   Plasmid

   #160296

   Purpose

   Yeast CRISPR plasmid targeting the natMX cassette

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
   URA3 selection cassette
   sgRNA expression cassette (without guide)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCY5k

   Plasmid

   #160294

   Purpose

   Yeast CRISPR plasmid targeting the kanMX cassette

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
   URA3 selection cassette
   sgRNA expression cassette (without guide)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEG302-SunTagng-22aa

   Plasmid

   #115345

   Purpose

   Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome

   Depositor

   Steven Jacobsen

   Insert

   NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiCRISPRv2-ACTB-C4

   Plasmid

   #229849

   Purpose

   Expresses wild-type Cas9 and gRNA for ACTB gene. The target sequence is changed from ACTB-C1 to improve the cut efficiency.

   Depositor

   Natsuko Chiba

   Insert

   guide RNA for ACTB gene
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCY5.1nh

   Plasmid

   #160299

   Purpose

   Yeast CRISPR plasmid targeting the natMX and hphMX cassettes

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
   URA3MX selection cassette
   sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCY5.1kn

   Plasmid

   #160298

   Purpose

   Yeast CRISPR plasmid targeting the kanMX and natMX cassettes

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
   URA3MX selection cassette
   sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lenti-SAM-Cre

   Plasmid

   #198714

   Purpose

   For expression of guide RNAs, the SAM transcriptional activator for CRISPRa and Cre in mammalian cells

   Depositor

   Tyler Jacks

   Article

   Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

   Inserts

   U6-MS2-gRNAscaffold
   Pgk-MPH-Cre
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pgk and U6

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

   Plasmid

   #149426

   Purpose

   The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Inserts

   U6.3-gRNAs[Sxl, bTub]
   Hsp70Bb-Cas9-T2A-eGFP-p10
   

   Use

   CRISPR

   Tags

   eGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. 1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]

    Plasmid

    #149427

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Inserts

    U6.3-gRNAs[TraB, bTub]
    Hsp70Bb-Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge