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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 19,618 results for: EGFP

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Showing: 6341 - 6360 of 19618 results

  2. pAAV-Dlx5/6-Chronos-eGFP-p2a-nls-mScarlet

    Plasmid
    #121543
    Purpose
    Chronos with red nucleus-targeted fluorescence for inhibitory neurons
    Depositor
    Matthias Prigge
    Insert
    blue-absorbing channelrhodopsin Chronos with eGFP and nls-mScarlet
    Use
    AAV
    Promoter
    dlx5/6
    Available since
    Feb. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    121543-plasmid-map-sequence-id-235975

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  4. pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

    Plasmid
    #185492
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Publication
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMV and T7
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185492-plasmid-map-sequence-id-363405

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  5. pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

    Plasmid
    #223123
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9=D10A
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223123-plasmid-map-sequence-id-447704

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  6. GOSR2-eGFP

    Plasmid
    #209886
    Purpose
    Expresses human GOSR2 (GS27) fused to eGFP in mammalian cells
    Depositor
    Geert van den Bogaart
    Insert
    GOSR2 (GOSR2 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    209886-plasmid-map-sequence-id-413667

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  10. pHSV-ELGA1

    Plasmid
    #246926
    Purpose
    Bioluminescent endolysosomal Ca2+ indicator lower Ca2+ affinity
    Depositor
    Teresa Alonso
    Publication
    Calvo et al J Cell Biol. 2025 Dec 1;224(12):e2024100
    Insert
    ELGA1 (Endo-lysosome GFP-Aequorin)
    Use
    Herpes viral vector
    Expression
    Mammalian
    Mutation
    D117A, D119A, D163A in aequorin gene
    Promoter
    IE4/5 promoter (HSV)
    Available since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246926-plasmid-map-sequence-id-492862

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  12. pCMV-T7-ABE8e-SpCas9(LWKYQS)-P2A-EGFP (AHK404)

    Plasmid
    #223113
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(LWKYQS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/LWKYQS)=D1135L, S1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223113-plasmid-map-sequence-id-447636

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  13. pOCC94

    Plasmid
    #118912
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    Tags
    MBP, cleavable with 3C protease and eGFP-HIS6
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118912-plasmid-map-sequence-id-232258

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  17. pEGFP-Kv2.2

    Plasmid
    #231678
    Purpose
    Expression of mouse Kcnb2 fused with EGFP on N-terminal.
    Depositor
    Federico Sesti
    Insert
    Kcnb2 (Kcnb2 Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    None
    Available since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231678-plasmid-map-sequence-id-463617

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  18. KA801: pMVP (L3-L2) HA tag + pA; CMV::eGFP-P2A-TETa-pA

    Plasmid
    #121800
    Purpose
    pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven GFP-P2A-TETa
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    HA epitope tag-polyA + CMV::eGFP-P2A-TETa-polyA
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121800-plasmid-map-sequence-id-237308

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Showing: 6341 - 6360 of 19618 results