We narrowed to 19,003 results for: Tec

1. pU6-Sp-gRNA-IDS_DF_D1b_attB_rev

   Plasmid

   #182152

   Purpose

   To insert attB-rev attachment site at IDS in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   IDS-D1b-attB-rev pegRNA
   

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-Sp-gRNA-IDS_DF_C2c_attB_rev

   Plasmid

   #182151

   Purpose

   To insert attB-rev attachment site at IDS in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   IDS-C2c-attBrev pegRNA
   

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-Sp-gRNA-IDS2_DF_B7b_attP_fwd

   Plasmid

   #182150

   Purpose

   To install attP-fwd attachment site at IDS2 in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   IDS2-B7b-attP-fwd pegRNA
   

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-CMV-H2B-FusionRed

   Plasmid

   #164430

   Purpose

   AAV plasmid expressing nuclear-localized FusionRed fluorophore

   Depositor

   Connie Cepko

   Article

   Wang et al Mol Ther Methods Clin Dev. 2020 Oct 10;1

   Insert

   H2B-FusionRed
   

   Use

   AAV

   Tags

   H2B

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. TAL2268

   Plasmid

   #36705

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   FOXO3-TALEN_Left (Foxo3 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

6. TAL2269

   Plasmid

   #36706

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   FOXO3-TALEN_Right (Foxo3 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pOCC92

   Plasmid

   #118910

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal eGFP-HIS6

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HBMss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
   

   Tags

   eGFP-HIS6

   Expression

   Insect

   Promoter

   polH

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMM0647

   Plasmid

   #129008

   Purpose

   Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   KanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
   

   Expression

   Yeast

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOCC6

   Plasmid

   #118864

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal EGFP

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-EGFP-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease, EGFP

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB3043(gRNA XI-1)

    Plasmid

    #73285

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOCC204

    Plasmid

    #118894

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C and C-terminal mKate2, and HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease and mKate2, HIS6,…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. TAL2382

    Plasmid

    #36791

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    AKT2-TALEN_Left (Akt2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pG.Lantern-SM50-GFP

    Plasmid

    #135593

    Purpose

    Expression of SM50 Promoter-GFP mRNA for microinjection to visualize SM50 promoter activity in S. purpuratus

    Depositor

    Koji Akasaka, Mamiko Yajima

    Article

    Yajima et al Dev Genes Evol. 2007 Apr;217(4):331-6. d

    Insert

    SM50 promoter (SM50 S. purpuratus)
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    Green Lantern GFP

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOCC81

    Plasmid

    #118900

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C , and N-terminal GST

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-GST-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease; GST tag

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCfB3049(gRNA XII-4)

    Plasmid

    #73291

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSET FLIPglu-600uDelta11 Ares-Aphrodite

    Plasmid

    #13571

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta11 Aphrodite
    

    Tags

    Aphrodite and Ares

    Expression

    Bacterial

    Mutation

    Fluorophore-derived linker shortened. F16A mutati…

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. TAL2450

    Plasmid

    #36839

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    HMGA2-TALEN_Left (Hmga2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

18. CD4_LD 0_CS G_tTA-BFP

    Plasmid

    #58869

    Purpose

    MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TAL2208

    Plasmid

    #36671

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    HOXA13-TALEN_Left (HOXA13 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

20. TAL2307

    Plasmid

    #36735

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    ERCC2-TALEN_Right (Ercc2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only