We narrowed to 9,378 results for: mel

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SGyA

Plasmid

#160292

Purpose

Drives expression of Cas9 and a TdTomato fluorescent marker

Depositor

Omar Akbari

Article

Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:

Inserts

Vas promoter
hSpCas9
tdTomato
CTCF insulator
Gypsy insulator
Left homology arm Y chromosome
Right homology arm Y chromosome

Tags

T2A GFP

Expression

Insect

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mCerulean 156-239-GGGS

Plasmid

#162616

Purpose

Allows for transcription of control mCerulean fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mCerulean

Expression

Mammalian

Mutation

156-239

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-CLASP2 512-650

Plasmid

#24409

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (512-650) (CLASP2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

CLASP2 deletion mutant with only MT plus end trac…

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarG

Plasmid

#31221

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Insert

CD4-tdGFP (CD4 Aequorea victoria, Human, Fly)

Tags

CD4 and EGFP/GFP

Expression

Insect

Mutation

Fusion of signal peptide of Drosophila Akh gene, …

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pGEX-TEVsite-human Glomulin

Plasmid

#52292

Purpose

bacterial expression of human Glomulin

Depositor

Brenda Schulman

Article

Duda et al Mol Cell. 2012 Aug 10;47(3):371-82. doi:

Insert

Glomulin (GLMN Human)

Tags

GST and TEV

Expression

Bacterial

Promoter

tac

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pAWG-dCas9-VPR

Plasmid

#63802

Purpose

SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vector

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

SP-dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Insect

Promoter

act5c

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
myr-FLAG-PIK3CA-pcw107

Plasmid

#64605

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

PIK3CA (PIK3CA Bovine)

Use

Lentiviral

Tags

FLAG and myristoylated

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pLEX307-IBV-3CL

Plasmid

#160288

Purpose

Allows for constituitive mammalian expression of the IBV 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR2 DD_NES

Plasmid

#124706

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD_NES (RAB7A Human)

Use

AAV

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRN3P-HA-Suv4-20h1mut

Plasmid

#86690

Purpose

For transcription of of Suv4-20h1mut mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Article

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5B mutant (Kmt5b Mouse)

Tags

HA

Mutation

mutated region NHDC (asparagine 273 to cysteine 2…

Promoter

T3

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR1 DD_NES

Plasmid

#124630

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with MCP_ADAR1 DD_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR1 DD_NES (RAB7A Human)

Use

AAV

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MED7

Plasmid

#15416

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED7 (MED7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pRecLV105

Plasmid

#107238

Purpose

A lentiviral vector for expression in mammalian cells

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pET-Sac-Aβ(M1-42/C18C33)

Plasmid

#173759

Purpose

Expresses the AβC18C33 peptide.

Depositor

James Nowick

Article

Zhang et al Biochemistry. 2022 Feb 15;61(4):252-264.

Insert

Aβ(M1-42/C18C33) (APP Human)

Expression

Bacterial

Mutation

changed valine 18 to cysteine; changed glycine 33…

Promoter

T7

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3XHA-Zmat3

Plasmid

#167343

Purpose

Expresses HA-tagged Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

Zinc finger matrin-type 3 (Zmat3 Mouse)

Tags

3HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX307-HCoV-NL63-3CL

Plasmid

#160286

Purpose

Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-Bat-CoV-HKU9-3CL

Plasmid

#160284

Purpose

Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA

Plasmid

#217511

Purpose

KD Recombinase dependent conditional smGdP-10XHA cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA

Tags

smGdP-10XHA

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-TWIN-STREP-FLAG-FLCN

Plasmid

#164981

Purpose

FLCN expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

FLCN (FLCN Human)

Tags

2xSTREP FLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

Plasmid

#217512

Purpose

KD Recombinase dependent conditional smGdP-10XOllas cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

Tags

smGdP-10XOllas

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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