Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 5,008 results for: AAT

Showing: 621 - 640 of 5008 results

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pUC57-ITR2-H1-GFP sgRNA Target 2- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

Plasmid

#210738

Purpose

Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 2

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

Sp Cas9 (cas9 S pyogenes)
Sp sgRNA GFP Target 2

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Promoter

H1 and eCMV

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-GFP sgRNA Target 4- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

Plasmid

#210740

Purpose

Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 4

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

Sp Cas9 (cas9 S pyogenes)
Sp sgRNA GFP Target 4

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Promoter

H1 and eCMV

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-GFP sgRNA Merry Target 2- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

Plasmid

#210742

Purpose

Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 2

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

SaSp Cas9
Merry sgRNA GFP Target 2

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Mutation

N-terminal Sa Cas9, C-terminal Sp Cas9

Promoter

H1 and eCMV

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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tet pLKO.1-shNUP37 v1 puro

Plasmid

#192343

Purpose

Lentiviral expression vector for an inducible shNUP37 v1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

shNUP37 v1 (NUP37 Human)

Use

Lentiviral

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAP01

Plasmid

#186260

Purpose

sfGFP under light light responsive PEL222 promoter

Depositor

Yongku Cho

Article

Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

Insert

sfGFP

Expression

Bacterial

Promoter

PEL222 (GGTAGCCTTTAGTCCATGTTAGCGAAGAAAATGGTTTGTTA…

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_MTOR

Plasmid

#183311

Purpose

All-in-One CRISPRko system with a guide RNA that targets MTOR gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

MTOR

Use

Lentiviral

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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shRNA-Ratio-NegCon

Plasmid

#183554

Purpose

Designed to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio negative control (Luciferase), Fig S2)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Luciferase shRNA

Expression

Mammalian

Promoter

CBh

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_YES1

Plasmid

#183329

Purpose

All-in-One CRISPRko system with a guide RNA that targets YES1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

YES1

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNArodA

Plasmid

#149658

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-PGRMC1-1-54

Plasmid

#173472

Purpose

PGRMC1 lacking the C-terminal region downstream of the transmembrane domain

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1 (PGRMC1 Human)

Tags

HA

Expression

Mammalian

Mutation

Deleted for amino acids 55-194

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-PGRMC1-21-195

Plasmid

#173473

Purpose

PGRMC1 lacking the N-terminal region upstream of the transmembrane region

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1 (PGRMC1 Human)

Tags

HA

Expression

Mammalian

Mutation

Deleted for amino acids 1-20

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNAflaB

Plasmid

#149561

Purpose

flaB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_PflaBS-sgRNAflaB

Plasmid

#149643

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, PflaBS

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNArodA

Plasmid

#149569

Purpose

rodA gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0826L-sgRNAflaB

Plasmid

#149648

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0826L

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNArodA

Plasmid

#149655

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_PresTS-sgRNAflaB

Plasmid

#149644

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, PresTS

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_PresTL-sgRNAflaB

Plasmid

#149645

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, PresTL

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0026-sgRNAflaB

Plasmid

#149646

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0026

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9S_P0826S-sgRNAflaB

Plasmid

#149647

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0826S

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 621 - 640 of 5008 results

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