Addgene

We narrowed to 1,799 results for: PORA

Showing: 621 - 640 of 1799 results

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msFam160b1 (msFHIP2A) g1 lentiCRISPRv2-mCherry

Plasmid

#218654

Purpose

Knockout vector for mouse Fam160b1 (Fhip2A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Fam160b1 (Fhip2a Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP)

Plasmid

#218640

Purpose

Gateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Tags

Expression

Mutation

delPYD (I125M-END)

Promoter

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 hNLRP3 R262W (disease-associated mutation)

Plasmid

#218647

Purpose

Gateway shuttling vector for cloning NLRP3-R262W (no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (NLRP3 Human)

Use

Gateway entry

Tags

Expression

Mutation

R262W

Promoter

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3(ΔPYD, I125M-END)

Plasmid

#218641

Purpose

Gateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Tags

Expression

Mutation

delPYD (I125M-END)

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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msFam160b1 (msFHIP2A) g2 lentiCRISPRv2 mCherry

Plasmid

#218655

Purpose

Knockout vector for mouse Fam160b1 (Fhip2A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Fam160b1 (Fhip2a Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A)

Plasmid

#218636

Purpose

Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

H781E, Q782R, F785A

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist Amp msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template

Plasmid

#215000

Purpose

Endogenously tag mouse TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template (Tmem115 Mouse)

Use

Hdr template

Tags

3xHA

Expression

Mutation

Promoter

None

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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6xABRE_A

Plasmid

#185803

Purpose

A synthetic ABA signaling reporter designed by multimerization of ABRE (7bp) and its flanking sequences from the ABI1 promoter

Depositor

Jose Dinneny

Article

Wu et al Plant Physiol. 2018 Aug;177(4):1650-1665

Insert

6xABRE(ABI1)+-86MP+erGFP

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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β2AR-PPI-CALPon1.0 (TEVp)

Plasmid

#213407

Purpose

Peroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 2)

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

HA-arrestin-TEVp

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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6xABRE_R

Plasmid

#185804

Purpose

a synthetic ABA signaling reporter designed by multimerization of ABRE (7bp) and its flanking sequences from the RD29A promoter

Depositor

Jose Dinneny

Article

Wu et al Plant Physiol. 2018 Aug;177(4):1650-1665

Insert

6xABRE(RD29A)+-86MP+erGFP

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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nUnaG-ST

Plasmid

#207630

Purpose

A plasmid encoding the N-terminal UnaG fragment fused to SpyTag.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

nUnaG-SpyTag-MBP

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-84

Promoter

T7

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_g5-HT2m

Plasmid

#208710

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2m in mammalian cells

Depositor

Yulong Li

Article

Deng et al bioRxiv 2023.05.27.542566

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2m

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_r5-HTmut

Plasmid

#208714

Purpose

Expresses the red 5-HT sensor GRAB_r5-HTmut in mammalian cells

Depositor

Yulong Li

Article

Deng et al bioRxiv 2023.05.27.542566

Insert

Red fluorescent 5-HT sensor GRAB_r5-HTmut

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_g5-HT3.0mut

Plasmid

#208712

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in mammalian cells

Depositor

Yulong Li

Article

Deng et al bioRxiv 2023.05.27.542566

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-NcPAN3_234-656-GW-mut_U

Plasmid

#147681

Purpose

Bacterial Expression of NcPAN3_234-656-GW-mut

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

NcPAN3_234-656-GW-mut

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_506-938-D603K_M

Plasmid

#146962

Purpose

Bacterial Expression of NcQde-2_506-938-D603K

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-D603K

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_506-938-K638E_M

Plasmid

#146963

Purpose

Bacterial Expression of NcQde-2_506-938-K638E

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-K638E

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-A602E_M

Plasmid

#146964

Purpose

Bacterial Expression of NcQde-2_506-938-A602E

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-A602E

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-H609W_M

Plasmid

#146965

Purpose

Bacterial Expression of NcQde-2_506-938-H609W

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-H609W

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-K634A_M

Plasmid

#146966

Purpose

Bacterial Expression of NcQde-2_506-938-K634A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-K634A

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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