We narrowed to 2,317 results for: SE

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pHH0103_PFN2_Profilin

Plasmid

#110007

Purpose

Protein expression and purification of PFN2_Profilin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PFN2_Profilin (PFN2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_UEVLD_UEV

Plasmid

#109902

Purpose

Protein expression and purification of UEVLD_UEV

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

UEVLD_UEV (UEVLD Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pcoCASphi_E9t_MCS_version2

Plasmid

#197950

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pcoCasphi-2

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLib6.4B

Plasmid

#176665

Purpose

Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_XRCC4_XRCC4

Plasmid

#110047

Purpose

Protein expression and purification of XRCC4_XRCC4

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

XRCC4_XRCC4 (XRCC4 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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p-mCherry2-sgC9cen

Plasmid

#198326

Purpose

Expresses a guide RNA against a repetitive locus found in the pericentromere of human chromosome 9, with mCherry2 reporter

Depositor

Sarah McClelland

Article

Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

Insert

Chr9-CEN guide RNA

Expression

Mammalian

Promoter

hU6

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSL0721 (pDonor_L1-105)

Plasmid

#130645

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened left end. Total transposon size = 933 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (short L)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLib6.6B

Plasmid

#176666

Purpose

Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSL0376 (His-MBP-StrepII-TniQ)

Plasmid

#130641

Purpose

Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite-StrepII for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite-StrepII on TniQ

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-EAF pBacPAK8

Plasmid

#15323

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

ELL Associated Factor (EAF1 Human)

Use

Baculovirus construction

Tags

6x His and FLAG

Promoter

polyhedrin

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0121

Plasmid

#177053

Purpose

MoClo Level 1, position 2, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0117

Plasmid

#177049

Purpose

MoClo Level 1, position 6, transcriptional unit for transient expression of iridoid synthase (CrISY) from Catharanthus roseus driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

iridoid synthase (CrISY) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T2 SHP2 D61A

Plasmid

#8323

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

D61A (activated mutant)

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
pSL0715 (pDonor_R1-57)

Plasmid

#130644

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened right end. Total transposon size = 910 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (short R)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_SRPK2_Pkinase

Plasmid

#110026

Purpose

Protein expression and purification of SRPK2_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SRPK2_Pkinase (SRPK2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET32b-FLJ23445

Plasmid

#15434

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

FLJ23445

Tags

His and Trx

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pMC_mNG2(11)_BSDminus_F1

Plasmid

#184163

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(11)
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-ELL2 pBacPAK8

Plasmid

#15327

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

Eleven Nineteen Lysine Rich in Leukemia 2 (ELL2 Human)

Use

Baculovirus construction

Tags

FLAG

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pEPQD1CB0108

Plasmid

#177041

Purpose

MoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geranyl pyrophosphate synthase (PaGPPS1) from Picea abies

Use

Synthetic Biology

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Cquin_596

Plasmid

#176669

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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