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pR

Plasmid

#193746

Purpose

Expresses LuxR under the control of BBa_J23102 constitutive promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al ACS Synth Biol. 2021 Oct 15;10(10):2456-

Insert

LuxR

Expression

Bacterial

Promoter

BBa_J23102

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pWSK29-wzzBST

Plasmid

#172968

Purpose

pWSK29 with Salmonella typhimurium wzzBST gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzzB gene from Salmonella enterica serovar Typhimurium

Expression

Bacterial

Mutation

K198E- please see depositor comments

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK29-fepEST

Plasmid

#172969

Purpose

pWSK29 with Salmonella typhimurium fepEST gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

fepE gene from Salmonella enterica serovar Typhimurium

Expression

Bacterial

Mutation

G160S- please see depositor comments

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLIC.B3.MJ0130m

Plasmid

#11528

Depositor

Sung-Hou Kim

Article

Kim et al Proc Natl Acad Sci U S A. 2005 Mar 1. 10

Insert

Type I restriction-modification enzyme (MJ_0130m M. jannaschii)

Tags

HisTag, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library 1

Pooled Library

#168776

Purpose

Pooled library expressing barcoded SARS-CoV-2 RNA Binding Domain mutations

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Expression

Yeast

Species

Sars-cov-2

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library 2

Pooled Library

#168777

Purpose

Pooled library expressing barcoded SARS-CoV-2 RNA Binding Domain mutations

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Expression

Yeast

Species

Sars-cov-2

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only
pT3-Neo

Plasmid

#69123

Purpose

Sleeping Beauty (SB) transposon with G418 resistance

Depositor

Martin Bonamino

Article

Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

Insert

Neo (codon optimized for human)

Use

Sleeping beauty transposon

Expression

Mammalian

Promoter

U3MSCV

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pPB_mU6_enh-gRNA_Puro-T2A-BFP

Plasmid

#235571

Purpose

Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

sgRNA backbone

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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FokI-dCas9

Plasmid

#52970

Purpose

Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

Depositor

David Liu

Article

Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

Fok1 fused to dCas9

Use

CRISPR

Tags

Fok1 nuclease (homodimeric wildtype) and NLS

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pRS156

Plasmid

#182707

Purpose

CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

Depositor

Rebecca Shapiro

Article

Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pT3-Neo-EF1a-GFP

Plasmid

#69134

Purpose

Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

Depositor

Martin Bonamino

Article

Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

Type

Empty backbone

Use

Sleeping beauty transposon

Expression

Mammalian

Promoter

U3MSCV

Available Since

Jan. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-KIRIN1

Plasmid

#107416

Purpose

Mammalian cell expression plasmid for potassium ion ratiometric indicator KIRIN1 with nuclear exclusion sequence

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1

Tags

Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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Tom20-CIB-GFP

Plasmid

#117242

Purpose

Light activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

CIBN

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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LOTUS-V/pcDNA3

Plasmid

#87127

Purpose

Bioluminescent and ratiometric voltage indicator

Depositor

Takeharu Nagai

Article

Inagaki et al Sci Rep. 2017 Feb 13;7:42398. doi: 10.10

Insert

LOTUS-V

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pTA-HUH

Plasmid

#221152

Purpose

HisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolytica

Depositor

Sheng Yang

Article

Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

Insert

HisG-URA3-HisG

Expression

Bacterial and Yeast

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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35S::GeNL/pCAMBIA1301

Plasmid

#190070

Purpose

Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana

Depositor

Takeharu Nagai

Article

Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

Insert

Green-enhanced Nano-lantern

Expression

Plant

Promoter

CaMV 35S

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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Mito-Lapronic/pGW1

Plasmid

#140754

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Mito-Lapronic

Tags

Mitochondrial Sequence Signal

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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Cry2(1-531)-mCh-BAXS184E

Plasmid

#117238

Purpose

Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

Cry2531-mCh-BAXS184E

Expression

Mammalian

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pENO1-iRFP-NATr

Plasmid

#129731

Purpose

Integration plasmid to overexpress iRFP in Candida albicans

Depositor

Robert Wheeler

Article

Bergeron et al Infect Immun. 2017 Oct 18;85(11). pii: I

Insert

iRFP-Candida albicans codon-optimized

Expression

Yeast

Promoter

ENO1 from Candida albicans

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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BRAC/pcDNA3

Plasmid

#51967

Purpose

mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicator

Depositor

Takeharu Nagai

Article

Saito et al PLoS One. 2010 Apr 1;5(4):e9935. doi: 10

Insert

BRAC

Expression

Mammalian

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only