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pLV-SFFV-myc-STIM1-WPRE-UbC-Emerald

Plasmid

#225939

Purpose

Lentiviral vector plasmid expressing human stromal interaction molecule 1 (STIM1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Richard Eva, Bart Nieuwenhuis, Evan Reid

Inserts

STIM1 (STIM1 Human)
Emerald

Use

Lentiviral

Tags

myc-tag

Expression

Mammalian

Promoter

SFFV and UbC

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-myc-STIM2-WPRE-UbC-Emerald

Plasmid

#225940

Purpose

Lentiviral vector plasmid expressing human stromal interaction molecule 2 (STIM2) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Richard Eva, Bart Nieuwenhuis, Evan Reid

Inserts

STIM2 (STIM2 Human)
Emerald

Use

Lentiviral

Tags

myc-tag

Expression

Mammalian

Promoter

SFFV and UbC

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-myc-ATL1-WPRE-UbC-Emerald

Plasmid

#225944

Purpose

Lentiviral vector plasmid expressing human atlastin GTPase 1 (ATL1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Richard Eva, Bart Nieuwenhuis, Evan Reid

Inserts

ATL1 (ATL1 Human)
Emerald

Use

Lentiviral

Tags

myc-tag

Expression

Mammalian

Promoter

SFFV and UbC

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-NEK9shRNA2

Plasmid

#220555

Purpose

To inducibly knockdown NEK9 expression

Depositor

Sean Lee

Insert

NEK9 shRNA2 (NEK9 Human)

Use

Lentiviral

Promoter

TRE3G (TetOP) promoter

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-NEK8shRNA1

Plasmid

#220552

Purpose

To inducibly knockdown NEK8 expression

Depositor

Sean Lee

Insert

NEK8 shRNA1 (NEK8 Human)

Use

Lentiviral

Promoter

TRE3G (TetOP) promoter

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-NEK8shRNA2

Plasmid

#220553

Purpose

To inducibly knockdown NEK8 expression

Depositor

Sean Lee

Insert

NEK8 shRNA2 (NEK8 Human)

Use

Lentiviral

Promoter

TRE3G (TetOP) promoter

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-NEK9shRNA1

Plasmid

#220554

Purpose

To inducibly knockdown NEK9 expression

Depositor

Sean Lee

Insert

NEK9 shRNA1 (NEK9 Human)

Use

Lentiviral

Promoter

TRE3G (TetOP) promoter

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEN_hU6miR-G12-E

Plasmid

#25757

Purpose

Entry vector with human U6 promoter driving mouse G alpha 12 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 12 miR-shRNA (Gna12 Mouse)

Use

Entry vector

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pEN_TmiR_G12-E

Plasmid

#25761

Purpose

Entry vector with TRE promoter driving mouse G alpha 12 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 12 miR-shRNA (Gna12 Mouse)

Use

Entry vector

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pEN_hU6miR-Gb2-K

Plasmid

#25759

Purpose

Entry vector with human U6 promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 miR-shRNA (Gnb2 Mouse)

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pSCAR_Cas9-blast_GFP

Plasmid

#162074

Purpose

3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Inserts

Cas9-2a-blasticidin S deaminase
EGFP

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Mutation

codon-optimized for expression in M. musculus

Promoter

EF1a and sv40

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSCAR_Cas9-hygro_GFP

Plasmid

#162075

Purpose

3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Inserts

Cas9-2a-hygromycin B phosphotransferase
EGFP

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Mutation

codon-optimized for expression in M. musculus

Promoter

EF1a and sv40

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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Plasmids for Multiplexed Optical Sensors Arrayed in Islands of Cells (MOSAIC)

Plasmid Kit

#1000000175

Purpose

Plasmids and Gateway entry vectors for fluorescent sensors of diverse physiological parameters in cells.

Depositor

Adam Cohen

Application

Gene Expression and Labeling

Vector Type

Lentiviral, Mammalian Expression

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only
LentiCRISPRv2-mCherry

Plasmid

#99154

Purpose

Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

Depositor

Agata Smogorzewska

Inserts

Cas9
mCherry

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS-NS

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pUBC-Pfv-Sapphire_PURO

Plasmid

#203651

Purpose

Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a puromycin selection marker.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

Lentiviral

Tags

Sapphire

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSMPUW_6.14_M4_Ef1a-HA-gateway-SV40-Zeo

Plasmid

#128450

Purpose

lentiviral destination vector with Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeo Resistance gene

Depositor

Lindy Barrett

Insert

Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeocin Resistance gene

Use

Lentiviral

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pUBC-SV40NLS-Pfv-Sapphire

Plasmid

#203653

Purpose

Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

Lentiviral

Tags

Sapphire

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_GFP_24

Plasmid

#238190

Purpose

Empty lentiviral vector for expressing protein sequences fused to GFP and FTH1 (24-mer), under a CMV promoter

Depositor

Feng Zhang

Type

Empty backbone

Use

Lentiviral

Tags

GFP-FTH1 and SV40

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-mCherry-WPRE-UbC-MCS

Plasmid

#211801

Purpose

Lentiviral vector plasmid expressing mCherry under the spleen focus-forming virus (SFFV) promoter and containing a multiple cloning site (MCS) under the Ubiquitin C (UbC) promoter

Depositor

Richard Eva, Bart Nieuwenhuis

Inserts

mCherry
multiple cloning site

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV and UbC

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pUltra-Plus-H2B-EGFP

Plasmid

#161776

Purpose

3rd generation lentiviral bicistronic vector for mammalian expression of H2B-EGFP and one gene of interest

Depositor

Jorge Luis Arias-Arias

Insert

H2B-EGFP from Addgene Plasmid #26790

Use

Lentiviral; Bicistronic

Tags

EGFP

Expression

Mammalian

Promoter

UbC and UbC promoter

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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