We narrowed to 9,242 results for: pho

1. pGEX-2T-RNAPII-CTD wt #14-HA

   Plasmid

   #160688

   Purpose

   Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

   Depositor

   Jeffry Corden

   Article

   Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

   Insert

   RNA Polymerase II CTD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C terminal domain only

   Promoter

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS406 ATG9 stopGFP

   Plasmid

   #114669

   Purpose

   Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

   Depositor

   Daniel Klionsky

   Article

   Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

   Insert

   ATG9
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Changed the three linker base pairs after the las…

   Promoter

   ATG9

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

   Plasmid

   #61966

   Purpose

   Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))

   Depositor

   Lynne Regan

   Article

   Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

   Inserts

   N-mCherry-ME(pS)VD
   TRAP(RK)-C-mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD and PLtetO

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Dapp1-PH pET11a

   Plasmid

   #71376

   Purpose

   Phosphoinositide Binding

   Depositor

   Mark Lemmon

   Article

   Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

   Insert

   Dapp1-PH (DAPP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa 148-273

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaPH

   Plasmid

   #22930

   Purpose

   Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PH domain

   Depositor

   Alexandra Newton

   Article

   Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

   Insert

   PHLPP1-deltaPH (PHLPP1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Includes 127-1205 aa. Deleted amino-terminal PH d…

   Promoter

   CMV

   Available since

   Feb. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

6. Grp1-PH pET11a

   Plasmid

   #71377

   Purpose

   Phosphoinositide Binding

   Depositor

   Mark Lemmon

   Article

   Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

   Insert

   Grp1-PH (Cyth3 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa 264-391

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pCX-2A-2A

   Plasmid

   #74670

   Purpose

   based on pCX-C1 with F2A1/F2A2 inserted

   Depositor

   Roberto Giovannoni

   Article

   De Giorgi et al Plasmid. 2015 May;79:22-9. doi: 10.1016/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGS

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pB-puro-V5-Trpt1

   Plasmid

   #126607

   Purpose

   Expresses N-terminal V5-tagged mouse Trpt1

   Depositor

   Xiaozhong Wang

   Article

   Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

   Insert

   tRNA 2' phosphotransferase 1 (Trpt1 Mouse)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET22-HT/prsA

   Plasmid

   #73624

   Purpose

   Produces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag

   Depositor

   James Williamson, T. J. Kappock

   Article

   Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

   Insert

   ribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAtipiTrc

    Plasmid

    #53275

    Purpose

    Contains an Arabidopsis thaliana idi1 cDNA fused to a strong Trc promoter. Produces an active isopentenyl diphosphate isomerase (IDI) in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al J Bacteriol. 2000 Oct;182(20):5841-8.

    Insert

    ipi1 (IPP2 Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Trc

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. F5A deltaCT S6K1 MSCV puro

    Plasmid

    #8464

    Depositor

    David Sabatini

    Article

    Ali et al J Biol Chem 2005 Apr 4;.

    Insert

    f5a delta ctp70 S6K1 (Rps6kb1 Rat)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    deletion of 104 c-terminal amino acids mutation …

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA-Dest53-SHP-1 S591D

    Plasmid

    #21803

    Depositor

    Stephen Shaw

    Article

    Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

    Insert

    SHP-1 (PTPN6 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed Serine 591 to Aspartic acid

    Promoter

    Available since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA-Dest53-SHP-1 S591A

    Plasmid

    #21802

    Depositor

    Stephen Shaw

    Article

    Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

    Insert

    SHP-1 (PTPN6 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed Serine 591 to Alanine

    Promoter

    Available since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pMEDS

    Plasmid

    #236568

    Purpose

    Introduces a multiple expression and dual selection (MEDS) system to use alongside CRISPR for applications such as gene editing.

    Depositor

    Shannon Moonah

    Article

    Sharma et al PLoS One. 2025 May 6;20(5):e0321881. doi

    Inserts

    CD/UPRT (negative selection)
    NPT II (positive selection)
    

    Use

    Tags

    3x Myc and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMx/ATP10D-HA

    Plasmid

    #209234

    Purpose

    Mammalian expression of ATP10D

    Depositor

    Hye-Won Shin

    Article

    Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

    Insert

    ATP10D (ATP10D Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSUMO chicken-cPLA2-C2-domain

    Plasmid

    #170753

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

    Insert

    PLA2G4A-C2 domain (PLA2G4A Chicken)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUNC2001

    Plasmid

    #67280

    Purpose

    Expresses MBP-Vibrio cholera photolyase in E. coli, vector pMalcw

    Depositor

    Aziz Sancar

    Article

    Worthington et al J Biol Chem. 2003 Oct 3;278(40):39143-54

    Insert

    Vibrio cholera photolyase
    

    Use

    Tags

    maltose binding protein

    Expression

    Bacterial

    Mutation

    none known

    Promoter

    Available since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST (AAV9)

    Viral Prep

    #105448-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST (#105448). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST plasmid DNA. hSyn-driven expression of ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Hillel Adesnik

    Article

    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

    Promoter

    Syn

    Tags

    mRuby2

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

19. FLAG-Cdk9 T186A

    Plasmid

    #28101

    Depositor

    Andrew Rice

    Article

    Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

    Insert

    Cdk9 (CDK9 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Contains a T186A mutation in Cdk9

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 6xHis-SNAP-PI(3,5)P2 Probe

    Plasmid

    #211512

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    SnxA-2xPX
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge