We narrowed to 12,024 results for: pcdna

1. pcDNA-GFP-EBA175-Flag(Y1425A)

   Plasmid

   #154973

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 Y1425A variant (residues 1284-1462) from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Use

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Mutation

   Y1425A; insert codes for EBA175 residues 1284 to …

   Promoter

   CMV

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-GFP-EBA175-Flag(Y1425I)

   Plasmid

   #154974

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 Y1425I variant (residues 1284-1462) from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Use

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Mutation

   Y1425I; insert codes for EBA175 residues 1284 to …

   Promoter

   CMV

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA-GFP-EBA175-Flag(Y1426A)

   Plasmid

   #154975

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 Y1426A variant (residues 1284-1462) from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Use

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Mutation

   Y1426A; insert codes for EBA175 residues 1284 to …

   Promoter

   CMV

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA-GFP-EBA175-Flag(Y1426I)

   Plasmid

   #154976

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 Y1426I variant (residues 1284-1462) from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Use

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Mutation

   Y1426I; insert codes for EBA175 residues 1284 to …

   Promoter

   CMV

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA4/TO-ORF24 202-752-3xFLAG

   Plasmid

   #138424

   Purpose

   Expresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   ORF24 202-752 (ORF24 KSHV (HHV-8))
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Amino acids 202-752

   Promoter

   CMV

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5 rtTA BirA eIF4G +MIC

   Plasmid

   #158790

   Purpose

   pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 including the microexon for generation of N2A FlpIn cell lines for BioID

   Depositor

   Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

   Insert

   eIF4G1 (with microexon)
   

   Use

   Tags

   3xFlag and BirA*

   Expression

   Mammalian

   Mutation

   Promoter

   miniCMV

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3/flag-pBirA(1-K283)-FRB

   Plasmid

   #154451

   Purpose

   expresses flag-pBirA(1-K283)-FRB/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   flag-pBirA(1-K283)-FRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)

   Plasmid

   #123340

   Purpose

   Negative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-EKAR-EV(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA8-Flag-LARS (361-720aa)

   Plasmid

   #139688

   Purpose

   Expresses N-teminal Flag tagged aa 361-720 of LARS1

   Depositor

   Jie Chen

   Article

   Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

   Insert

   LARS1 (LARS1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-mCherry-mCherry-FLARE-CKAR

    Plasmid

    #123345

    Purpose

    Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    mCherry-mCherry-FLARE-CKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Venus-cpVenus-FLARE-CKAR(T/A)

    Plasmid

    #123344

    Purpose

    Negative control mutant for Venus-cpVenus-FLARE-CKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-CKAR(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR

    Plasmid

    #123336

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-cpCerulean3-FLARE-AKAR
    

    Use

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-Cerulean3-Cerulean3-FLARE-AKAR

    Plasmid

    #123335

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-Cerulean3-FLARE-AKAR
    

    Use

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5 FRT-TO-EGFP-UPF1-R615A

    Plasmid

    #136001

    Purpose

    DOX-inducible R615A UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF1
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R615A

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5 FRT-TO-EGFP-UPF1-DEAA

    Plasmid

    #136002

    Purpose

    DOX-inducible DEAA UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF1
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    DE647AA

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA4/TO-ORF66 200-429-2xStrep

    Plasmid

    #130955

    Purpose

    Expresses KSHV ORF66 a.a. 200-429 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 200-429

    Promoter

    CMV

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hsa-miR-199-dendra2 PCDNA

    Plasmid

    #121133

    Purpose

    mammalian cell expression

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    hsa-miR-199
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(+)/CUG-stop-nLuc-3XFLAG

    Plasmid

    #127309

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon, but translation terminated immediately by stop codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1/NDUFS2 _34-66/myc-His

    Plasmid

    #127495

    Purpose

    Expresses myc-tagged _34-66 NDUFS2 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 _34-66 (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 _34-66

    Promoter

    CMV

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1/NDUFS2 E57A/myc-His

    Plasmid

    #127497

    Purpose

    Expresses myc-tagged NDUFS2/E57A in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 E57A (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 E57A

    Promoter

    CMV

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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