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pX330_gRNA_IG-DMR-2

Plasmid

#159932

Purpose

Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

Depositor

Anton Wutz

Article

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse IG-DMR

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6, CBh

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pPEE43

Plasmid

#139422

Purpose

Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mMaroon1 ORF and a flexible linker at the 5' end.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

mMaroon1

Expression

Yeast

Promoter

TEF1

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pPEE43

Plasmid

#139422

Purpose

Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mMaroon1 ORF and a flexible linker at the 5' end.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

mMaroon1

Expression

Yeast

Promoter

TEF1

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMtb MCM

Plasmid

#138296

Purpose

Expression of Mtb MCM small and large subunit

Depositor

Ruma Banerjee

Article

Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

Inserts

mutA
mutB

Tags

His tag and TEV cleavage site

Expression

Bacterial

Mutation

inserted TEV cleavage site between His-tag and m…

Promoter

T7

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pMtb MCM

Plasmid

#138296

Purpose

Expression of Mtb MCM small and large subunit

Depositor

Ruma Banerjee

Article

Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

Inserts

mutA
mutB

Tags

His tag and TEV cleavage site

Expression

Bacterial

Mutation

inserted TEV cleavage site between His-tag and m…

Promoter

T7

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pDSM-de-Phhf2-PYC-Tadh1

Plasmid

#127737

Purpose

Yeast pathway position 5. PYC transcription unit with the HHF2 promoter and ADH1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)

Use

Synthetic Biology

Expression

Yeast

Promoter

Phhf2

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pDSM-de-Phhf2-PYC-Tadh1

Plasmid

#127737

Purpose

Yeast pathway position 5. PYC transcription unit with the HHF2 promoter and ADH1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)

Use

Synthetic Biology

Expression

Yeast

Promoter

Phhf2

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgAspa.CMV/CB-EGFP

Plasmid

#121510

Purpose

Expresses sgRNA targeting mouse Aspa intron 2 (sgAspa).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgAspa

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgAspa.CMV/CB-EGFP

Plasmid

#121510

Purpose

Expresses sgRNA targeting mouse Aspa intron 2 (sgAspa).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgAspa

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-STOP-HEC1

Plasmid

#114026

Purpose

Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-STOP-HEC1 (NDC80 Human)

Expression

Mammalian

Mutation

Start codon of Hec1 is mutated to stop codon

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-STOP-HEC1

Plasmid

#114026

Purpose

Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-STOP-HEC1 (NDC80 Human)

Expression

Mammalian

Mutation

Start codon of Hec1 is mutated to stop codon

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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CKS2 C8.2 gRNA

Plasmid

#90633

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKS2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CKS2 (Guide Designation C8.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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CKS2 C8.2 gRNA

Plasmid

#90633

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKS2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CKS2 (Guide Designation C8.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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PPP1CC C4.5 gRNA

Plasmid

#90846

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP1CC

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP1CC (Guide Designation C4.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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PPP1CC C4.5 gRNA

Plasmid

#90846

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP1CC

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP1CC (Guide Designation C4.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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CAMSAP3 E8.5 gRNA

Plasmid

#90562

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMSAP3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CAMSAP3 (Guide Designation E8.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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CAMSAP3 E8.5 gRNA

Plasmid

#90562

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMSAP3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CAMSAP3 (Guide Designation E8.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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DYNLT1 B2.5 gRNA

Plasmid

#90669

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYNLT1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DYNLT1 (Guide Designation B2.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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DYNLT1 B2.5 gRNA

Plasmid

#90669

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYNLT1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DYNLT1 (Guide Designation B2.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMElmo2P1gfp

Plasmid

#90127

Purpose

Gateway Mid-entry clone containing promoter sequence specific for lmo2 driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

lmo2P1Egfp (lmo2 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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