We narrowed to 18,039 results for: emb
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Plasmid#48812Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorAvailable SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only
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pKK-FLAG-TEV
Plasmid#105768PurposeExpression of your protein of interest in fusion with FLAG at the N-terminus. The tag is cleavable by TEV protease or enterokinase.DepositorTypeEmpty backboneUseFlp-in competentTagsFLAG-TEVExpressionMammalianAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJET-07-mNeonGreen-R11a
Plasmid#179890PurposeDonor with homologous arms for Rab11a to knock in mNeonGreenDepositorInsertRAB11A, member RAS oncogene family (RAB11A Human)
UseCRISPR; Donor for homologous based knock inTagsmNeonGreenExpressionBacterial and MammalianAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-hPMCA2xb
Plasmid#47751Purposemammalian expression of mCherry tagged hPMCA2, xb splice variantDepositorInsertPMCA2x/b (ATP2B2 Human)
TagsmCherryExpressionMammalianMutationxb splice variantPromoterCMVAvailable SinceNov. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJET-41-DExCon-antiGFPnanobody-mCherry-R11b
Plasmid#179901PurposeDonor with homologous arms for Rab11b to knock in DExCon-antiGFPnanobody-mCherry moduleDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsantiGFPnanobody-mCherryExpressionBacterial and MammalianPromoterTRE3GSAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mEGFP
Plasmid#105794PurposeExpression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mEGFPExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-CRY2-P14P5K
Plasmid#79569Purposeexpression of GFP-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1cDepositorInsertPip5k1c (Pip5k1c Mouse)
TagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationR445E and R446E; truncation of amino acids 636-66…PromoterCMVAvailable SinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N3/hArf5(WT)-EGFP
Plasmid#79534PurposeExpresses C-terminally EGFP-tagged Arf5(WT) in mammalian cellsDepositorAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBrain-GFP-TACC3KDP(S558A)-shTACC3
Plasmid#59357PurposeExpresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.DepositorInsertTransforming Acidic Coiled Coil protein 3 (TACC3 Human)
UseRNAiTagsGFPExpressionMammalianMutationSilent mutations in amino acids 30-33 to confer s…PromoterCMVAvailable SinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
RCAS-sgATG7
Plasmid#75228PurposeAn adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor GammohDepositorAvailable SinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only