We narrowed to 12,189 results for: LEA

Showing: 6421 - 6440 of 12189 results

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SuExp-mPLD3

Plasmid

#201245

Purpose

express full length mouse PLD3 protein

Depositor

David Nemazee

Publication

Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

Insert

PLD3 (Pld3 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pet11a-ABLE

Plasmid

#158627

Purpose

Amp resistant IPTG inducible 6xHis-TEV-ABLE protein for E. coli expression

Depositor

William DeGrado

Publication

Polizzi et al Science. 2020 Sep 4;369(6508):1227-1233.

Insert

Apixaban-Binding Helical Bundle

Tags

6x His-tag and TEV cleavage tag

Expression

Bacterial

Promoter

T7

Available since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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ND6-L1397-N_UGI-NES_right

Plasmid

#187846

Purpose

expresses the right-side half ND6-UGI-NES in mammalian cells

Depositor

Chengqi Yi

Publication

Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

Insert

COX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–NES–ATP5B 3'UTR

Expression

Mammalian

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-perox-mCer

Plasmid

#185603

Purpose

Lentiviral construct for expression of mCerulean targeted to peroxisomes

Depositor

Jarrod Johnson

Publication

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

perox-mCer

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pET30-T7-lacO-His-sfGFP

Plasmid

#180754

Purpose

pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Publication

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Mutation

G140C- Please see depositor comments

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-FKBP-TEVp

Plasmid

#178867

Purpose

Mammalian expression of mCherry-FKBP-TEVprotease(Δ220-242)

Depositor

Shinya Tsukiji

Publication

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

mCherry-FKBP-TEVprotease(Δ220-242)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA(MS2)_zeo_hPDX1_promoter_guide1

Plasmid

#176838

Purpose

To induce human PDX1 expression by recruiting SAM complex to PDX1 promoter

Depositor

Rudolf Jaenisch

Publication

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

sgPDX1

Expression

Mammalian

Promoter

U6

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pJH809_pJH_LldR_Pcon-sfGFP.10TAG

Plasmid

#163739

Purpose

Constitutive expression of green fluorescent protein encoding ten stop codons

Depositor

Matthew Bennett

Publication

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.10TAG

Expression

Bacterial

Mutation

gfp with first 10 leucine codons mutated to amber

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH726_pJH_LldR_PlldR-sfGFP.15TAG

Plasmid

#163730

Purpose

Lactate-inducible expression of green fluorescent protein encoding fifteen amber codons

Depositor

Matthew Bennett

Publication

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.15TAG

Expression

Bacterial

Mutation

gfp with first 15 leucine codons mutated to amber

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc460

Plasmid

#161940

Purpose

Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter

Depositor

Baojun Wang

Publication

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI

Expression

Bacterial

Promoter

PPhlF

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc071

Plasmid

#161943

Purpose

Contains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmid

Depositor

Baojun Wang

Publication

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T

Expression

Bacterial

Promoter

PBAD

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-PK1Dexp

Plasmid

#130834

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Publication

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKG1

Tags

eGFP-FLAG-HA

Expression

Plant

Promoter

CaMV 35S

Available since

June 19, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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HM3C NaVSp1_p pET24b

Plasmid

#127852

Purpose

Bacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.

Depositor

Dan Minor

Publication

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVSp1

Tags

3C Cleavage site, His Tag, and MBP

Expression

Bacterial

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pME-actn2

Plasmid

#109550

Purpose

Multisite gateway vector for 5' tagging with alpha actinin 2

Depositor

Rob Parton

Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

alpha actinin 2

Use

Zebrafish plasmids

Mutation

Please see Depositor Comments

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pbIG-Xph20-ETWV

Plasmid

#133034

Purpose

Expresses Xph20-ETWV in bacteria

Depositor

Matthieu Sainlos

Publication

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph20-ETWV

Tags

AviTag, His10, and TEV protease cleavage site

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pSBbi-Pur-AktAR2

Plasmid

#125195

Purpose

SB-transposon plasmid for stable expression of AktAR2 variant of FRET-based AKT activity reporter

Depositor

Eric Davis

Insert

AktAR2

Use

Transposon

Tags

6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…

Expression

Mammalian

Promoter

EF1a/RPBSA

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b-YC

Plasmid

#107072

Purpose

Host vector to construct a YPer or CyPet fusion protein

Depositor

Teruyuki Nagamune

Publication

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

YPet-CyPet fusion protein

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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Tmem194 pEGFP-N2 (1074)

Plasmid

#62027

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Publication

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem194 (TMEM194A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
Tmem194 pmRFP-N2 (1389)

Plasmid

#62048

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Publication

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem194 (TMEM194A Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
myc-actin-NES-1

Plasmid

#60618

Purpose

Encodes MYC tagged beta-actin with a mutation in the NES

Depositor

Primal de Lanerolle

Publication

Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

Insert

Beta-actin (ACTB Human)

Tags

MYC

Expression

Bacterial and Mammalian

Mutation

I178A, L180A

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only