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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,067 results for: LEA

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Showing: 6421 - 6440 of 14067 results

  1. pENeGFP PCNAL2 pc653

    Plasmid
    #167564
    Purpose
    The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.
    Depositor
    Cristina Cardoso
    Publication
    Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Proliferating Cell Nuclear Antigen (PCNA Human)
    Tags
    GFP
    Expression
    Mammalian
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    167564-plasmid-map-sequence-id-388295

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  4. pTM

    Plasmid
    #162783
    Purpose
    Protein expression in CHO cells - hTPA leader and C-terminal c-myc and 10xhis tags are coded
    Depositor
    Ivan Vorobiev
    Publication
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Type
    Empty backbone
    Tags
    10xHis, c-myc, and hTPA leader
    Expression
    Mammalian
    Promoter
    CHO Elongation factor 1A
    Available since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162783-plasmid-map-sequence-id-333802

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  5. pETDuet-1 TIM8,13

    Plasmid
    #170282
    Purpose
    Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production
    Depositor
    Paul Schanda
    Publication
    Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea
    Insert
    yeast Tim8 and Tim13 (TIM8 Budding Yeast)
    Tags
    TEV-cleavable N-terminal His tag on Tim13 (on bac…
    Expression
    Bacterial
    Available since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170282-plasmid-map-sequence-id-334956

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  6. A-WDR5-1

    Plasmid
    #166515
    Purpose
    scFv of a human scaffold targeting WD repeat domain 5. Antigen coverage aa 1-334 of 334.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    April 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    166515-plasmid-map-sequence-id-327811

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  7. T-TRIM33-4

    Plasmid
    #166550
    Purpose
    scFv of a human scaffold targeting E3 ubiquitin-protein ligase TRIM33. Antigen coverage aa 882-1127 of 1127
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166550-plasmid-map-sequence-id-329241

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  8. G-SMARCA4-2

    Plasmid
    #166519
    Purpose
    scFv of a human scaffold targeting SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a4 isoform B. Antigen coverage aa 1451-1580 of 1647, Bromodomain.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166519-plasmid-map-sequence-id-329058

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  9. G-BRD4-4

    Plasmid
    #166518
    Purpose
    scFv of a human scaffold targeting Bromodomain-containing protein 4 isoform long. Antigen coverage aa 44-168 of 1362, Bromodomain 1.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166518-plasmid-map-sequence-id-329057

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  10. O-IL8-15

    Plasmid
    #166544
    Purpose
    scFv of a human scaffold targeting Interleukin-8. Antigen coverage aa 28-99 of 99, full-length excl. signal peptide
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166544-plasmid-map-sequence-id-328277

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  11. Z-UBQLN2-7

    Plasmid
    #166563
    Purpose
    scFv of a human scaffold targeting Ubiquilin-2. Antigen coverage aa 1-624 of 624 aa, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    166563-plasmid-map-sequence-id-327818

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  15. pCAG-T3-SpCAS9-NG-pA

    Plasmid
    #131465
    Purpose
    Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Publication
    Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon optimized SpCas9-NG
    Use
    CRISPR
    Tags
    Flag, NLS, and polyA
    Expression
    Mammalian
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131465-plasmid-map-sequence-id-262447

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  16. pAAV-CaMKIIa-ChRger1-TS-EYFP

    Plasmid
    #127244
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.
    Depositor
    Viviana Gradinaru
    Publication
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger1-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Promoter
    CamKIIa
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127244-plasmid-map-sequence-id-247231

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Showing: 6421 - 6440 of 14067 results