We narrowed to 15,981 results for: LEA

1. pSynapsin_NLS_GFP_P2A_SNAP_NR1

   Plasmid

   #170458

   Purpose

   Expresses SNAP tagged NR1 in neuronal cells

   Depositor

   Shigeki Watanabe

   Article

   Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

   Insert

   NR1 (Grin1 Rat)
   

   Use

   Lentiviral

   Tags

   SNAP

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. px459 sgAtg5

   Plasmid

   #175023

   Purpose

   CRISPR-Cas9 plasmid targeting exon 2 of human Atg5.

   Depositor

   David Tumbarello

   Article

   Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

   Insert

   ATG5 (ATG5 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension

   Plasmid

   #176542

   Purpose

   Bacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locus

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Inserts

   MBP tag
   TnpB
   

   Tags

   3' extension derived from endogenous locus a…

   Expression

   Bacterial

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-UAP56

   Plasmid

   #157661

   Purpose

   Expresses UAP56 in E.coli cell

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Spliceosome RNA helicase DDX39B (DDX39B Human)
   

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-43

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJRoC30_Th3.14

   Plasmid

   #188068

   Purpose

   FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.14)

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   FuncLib-designed high-redox potential laccase from Trametes hirsuta
   

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 PROSS + 4 FuncLib mutations, described in publ…

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TV-hPITX3-forward

   Plasmid

   #22073

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   PITX3 (ASMD Human)
   

   Use

   Cre/Lox; Zfn donor

   Tags

   eGFP

   Available Since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_UdgX-Anc689-UdgX-nCas9-RBMX

   Plasmid

   #163552

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-Anc689-UdgX-nCas9-RBMX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-SynI-FlpOn-CreOff-MCS-P2A-mCherry

   Plasmid

   #176277

   Purpose

   Viral vector for co-expression of a CDS of interest and mCherry in cells expressing Flp AND NOT Cre driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   MCS-P2A-mCherry
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   human Synapsin I

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMKO.1-shCcnb2

   Plasmid

   #162794

   Purpose

   Ccnb2 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Ccnb2 shRNA (Ccnb2 Mouse)
   

   Use

   RNAi and Retroviral

   Promoter

   U6

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. His-SUMO- flag-hDDI2-D252N_pET11a

    Plasmid

    #169123

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Expression

    Bacterial

    Mutation

    D252N

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. His-SUMO-flag-hDDI2_pET11a

    Plasmid

    #169121

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Expression

    Bacterial

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PITX3-eGFP-PGK-Puro

    Plasmid

    #31942

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Insert

    PITX3 (PITX3 Human)
    

    Use

    Targeting donor

    Tags

    eGFP

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLC-HA active Nedd8-P2A-Hygro

    Plasmid

    #124307

    Purpose

    Lentiviral vector for expression of HA tagged active or C-terminal cleaved Nedd8-P2A-Hygro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    Nedd8 (NEDD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Five C-terminal amino acids missing compared to w…

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21b-Caspase-3(D3A: D175A D28A D9A)

    Plasmid

    #90090

    Purpose

    Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

    Depositor

    Clay Clark

    Article

    Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D175A D28A D9A

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PET-B-eSpCas9-NLS-6xHis

    Plasmid

    #207383

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-eSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A, amino acids 1005-1013 repl…

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTER E2F1shRNA human

    Plasmid

    #66883

    Purpose

    Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F1

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F transcription factor 1 (E2F1 Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    H1 Tet

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pGP-CMV-GCaMP3-373

    Plasmid

    #91779

    Purpose

    Mammalian expression of low affinity calcium-sensing fluorescent protein

    Depositor

    GENIE Project, Douglas Kim

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    GCaMP3-373
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV_RBMX-eA3A-UdgX-nCas9

    Plasmid

    #163546

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    RBMX-eA3A-UdgX-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSFDuet-1_VNp-hGH

    Plasmid

    #182411

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-human growth hormone fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-hGH (GH1 Human)
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBactin-Halo-Tag

    Plasmid

    #196898

    Purpose

    Expression of Halo-Tag. Use as control for Halo-tag fusions

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Halo-Tag
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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