We narrowed to 11,695 results for: nar;

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DmTmem63

Plasmid

#136598

Purpose

Expresses the mechanosensitive channel Drosophila melanogaster Tmem63 in mammalian cells

Depositor

Ardem Patapoutian

Article

Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

Insert

DmTMEM63

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETB-de4GFP

Plasmid

#85472

Purpose

Inducible expression of dual emitting pH sensitive GFP in bacterial cells

Depositor

S. James Remington

Article

Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

Insert

de4GFP

Tags

6xHis

Expression

Bacterial

Mutation

S65T/C48S/H148C/T203C

Promoter

T7

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-GCaMP6s

Plasmid

#184712

Purpose

Calcium biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

GCaMP6s

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAD6

Plasmid

#134349

Purpose

E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

Depositor

Seth Darst

Article

Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

Insert

mfd (mfd E. coli)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBTK1024 (pDS-Def1)

Plasmid

#183125

Purpose

Expresses double-stranded RNA targeting the Def1 gene from Apis mellifera

Depositor

Jeffrey Barrick

Article

Lariviere et al Nat Protoc. 2023 Mar;18(3):902-928. doi:

Insert

Fragment of Def1 gene

Use

Synthetic Biology

Available Since

May 19, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBAV150

Plasmid

#79764

Purpose

binary DEST vector for plant expression

Depositor

Nico Dissmeyer, Jean Greenberg

Article

Vinatzer et al Mol Microbiol. 2006 Oct;62(1):26-44. Epu

Type

Empty backbone

Use

Tetr

Expression

Plant

Promoter

DEX

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pBTK1050

Plasmid

#209540

Purpose

BTK Type 1 homology arm for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Upstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region

Use

Synthetic Biology

Available Since

Feb. 9, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK1051

Plasmid

#209541

Purpose

BTK Type 5 homology arm for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Recombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region

Use

Synthetic Biology

Available Since

Feb. 2, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJPT003-PM-HaloTag-1x FLAG

Plasmid

#202494

Purpose

Express "PM" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Tags

1x FLAG and Palmitoylation-Myristoylation Site

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-W340A.pcDNA3-k

Plasmid

#127404

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon and W34…

Promoter

CMV

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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XW08

Plasmid

#65832

Purpose

Punc-4-QF-SL2-mcherry-3'UTR-unc-54

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

OR74A quinic acid utilization activator (qa-1F)

Tags

mCherry

Expression

Worm

Promoter

Punc-4

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSL-1

Plasmid

#180422

Purpose

Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a low level RFP promoter.

Depositor

Nancy Moran

Article

Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Red Chromprotein gene

Expression

Bacterial

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG_N2N3-HIS-ybbr

Plasmid

#101238

Purpose

SdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Article

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

SdrG_N2N3

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA1

Plasmid

#199727

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU3-sgRNA1 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA2

Plasmid

#199728

Purpose

Golden Gate entry vector; 2nd gRNA under OsU3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU3-sgRNA2 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pmNFM

Plasmid

#83126

Purpose

Mouse neurofilament protein M cDNA mammalian expression construct

Depositor

Anthony Brown

Article

Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

Insert

mouse NFM (Nefm Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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PHBA02

Plasmid

#83536

Purpose

Constitutive TEF1 promoter mediated expression of the UBiC and ARO4* genes

Depositor

Claudia Vickers

Article

Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

Insert

pTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t

Expression

Yeast

Promoter

pTEF1

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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XW52

Plasmid

#65838

Purpose

XW52 Pmig-13-Czipper-QF-AD-NLS-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

Czipper-QF-AD-NLS

Tags

mCherry

Expression

Worm

Promoter

Pmig-13

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pMJ915v2 + sfGFP

Plasmid

#88918

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-4493NES(Booster-PKA)

Plasmid

#184710

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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