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We narrowed to 6,481 results for: siae

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Showing: 6461 - 6480 of 6481 results

  1. PB-NES-ZapCV2

    Plasmid
    #222950
    Purpose
    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV2
    Tags
    ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222950-plasmid-map-sequence-id-450583

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  2. pb-NES-ZapCV5 (cpV143)

    Plasmid
    #112061
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV5
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CBA
    Available Since
    July 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  3. pLH_Scr15

    Plasmid
    #100537
    Purpose
    CEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    Phytochrome B (PHYB Mustard Weed)
    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    Use
    Cre/Lox and Synthetic Biology
    Tags
    CreC, CreN, and SV40 NLS
    Expression
    Yeast
    Mutation
    N-terminal version of PhyB (AA 1-621)/ Mutation o…
    Promoter
    FBA1 and TDH3
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    100537-plasmid-map-sequence-id-194456

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  4. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

    Plasmid
    #112060
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV2
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CMV
    Available Since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    112060-plasmid-map-sequence-id-216934

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  5. pRL_CT_GI_del1

    Plasmid
    #98744
    Purpose
    For red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Inserts
    phytochrome interacting factor 3 (PIF3 Mustard Weed)
    phytochrome B (PHYB Mustard Weed)
    Use
    Synthetic Biology
    Tags
    SV40-NLS, VP64-AD, and synTALE-DBD
    Expression
    Yeast
    Mutation
    mutated nucleotide 576 C to A, deleted amino acid…
    Promoter
    FBA1 and TDH3
    Available Since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98744-plasmid-map-sequence-id-189858

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  6. pLH_Scr16

    Plasmid
    #100538
    Purpose
    CEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    Phytochrome B (PHYB Mustard Weed)
    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Cdc1 NLS, CreC, CreN, and SV40 NLS
    Expression
    Yeast
    Mutation
    AA 1-621 of PhyB/ Mutation nucleotide 576 C-->A
    Promoter
    FBA1 and TDH3
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    100538-plasmid-map-sequence-id-194462

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  9. CEAS (C-SWAT for Essential Allele Strains)

    Plasmid Kit
    #1000000223
    Purpose
    Vectors used to lower gene expression in S. cerevisiae. Compatible with the genome-wide S. cerevisiae C-SWAT strain collection.
    Depositor
    Rob Mitra
    Application
    Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Available Since
    June 5, 2023
    Availability
    Academic Institutions and Nonprofits only

  10. Yeast Prototrophy Kit

    Plasmid Kit
    #1000000085
    Purpose
    Plasmids designed to restore prototrophy and test metabolic capacity in common S. cerevisiae laboratory strains.
    Depositor
    Markus Ralser
    Application
    Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Available Since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. EasyClone 2.0 Yeast Toolkit

    Plasmid Kit
    #1000000073
    Purpose
    An expanded toolkit of integrative vectors for stable gene expression in S. cerevisiae.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    USER
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. The Expanded EasyClone-MarkerFree toolkit

    Plasmid Kit
    #1000000190
    Purpose
    Expansion of the EasyClone-MarkerFree Vector Set (#1000000098) with eight additional chromosomal loci for the stable integration of heterologous DNA into Saccharomyces cerevisiae by CRISPR/Cas9.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    USER
    Available Since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  13. ScEnSor Kit

    Plasmid Kit
    #1000000215
    Purpose
    To investigate the intracellular environment of Saccharomyces cerevisiae with a variety of biosensors.
    Depositor
    Lisbeth Olsson
    Application
    Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Available Since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  14. pCut plasmid toolkit

    Plasmid Kit
    #1000000118
    Purpose
    Plasmids for integrating into well-characterized chromosomal loci to program gene expression in S. cerevisiae.
    Depositor
    Aindrila Mukhopadhyay
    Application
    Genome Editing
    Vector Type
    Yeast Expression
    Editing Type
    CRISPR
    Available Since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only

  15. The Expanded CRISPEY Kit

    Plasmid Kit
    #1000000257
    Purpose
    Use the Expanded CRISPEY Kit for CRISPR-based gene editing of the Saccharomyces cerevisiae genome.
    Depositor
    Jeffrey Lewis
    Application
    Genome Editing
    Vector Type
    Yeast Expression
    Editing Type
    CRISPR
    Available Since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only

  16. Yeast GoldenBraid Cloning System and Toolkit

    Plasmid Kit
    #1000000138
    Purpose
    This toolkit allows generation of transcriptional units using GoldenBraid for integration in the S. cerevisiae genome at loci, YPRCΔ15 and YORWΔ22, to support high and stable transgene expression.
    Depositor
    Elena Caro Bernat
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (GoldenBraid)
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  17. Yeast Secrete and Detect

    Plasmid Kit
    #1000000166
    Purpose
    Assembly of transcription units for protein secretion in the yeasts Saccharomyces cerevisiae and Pichia pastoris.
    Depositor
    Sylvestre Marillonnet
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  18. Easy-MISE toolkit

    Plasmid Kit
    #1000000230
    Purpose
    Easy-Modular Integrative fuSion-ready Expression toolkit allows fast metabolic engineering in Saccharomyces cerevisiae using CRISPR-Cas9 to insert TUs into well-defined genomic loci.
    Depositor
    Paola Branduardi
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Yeast Expression
    Editing Type
    CRISPR
    Cloning Type
    Golden Gate
    Available Since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only

  19. yeast Tunable Expression Systems Toolkit (yTEST)

    Plasmid Kit
    #1000000266
    Purpose
    Plasmids for assembly of small-molecule inducible promoter systems in Saccharomyces cerevisiae.
    Depositor
    Jeff Hasty
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only

  20. Multiplex Yeast Toolkit (MYT)

    Plasmid Kit
    #1000000229
    Purpose
    Collection of plasmids which extend the capabilities of the MoClo Yeast Toolkit (YTK) for genetically engineering S. cerevisiae.
    Depositor
    Tom Ellis
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Nov. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 6461 - 6480 of 6481 results