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MTK2_016

Plasmid

#123708

Purpose

Encodes truncated pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV-A

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_024

Plasmid

#123716

Purpose

Encodes crippled mouse pPGK1 as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pPGK1c

Expression

Mammalian

Mutation

Crippled Kozac sequence

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3_002

Plasmid

#123719

Purpose

Encodes bsr (blasticidin-S deaminase) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Blasticydin Resistance

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_027

Plasmid

#123689

Purpose

Encodes ConL5 and human E4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L5-E4 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_028

Plasmid

#123690

Purpose

Encodes ConL6 and human F1 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L6-F1 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_029

Plasmid

#123691

Purpose

Encodes ConL7 and human B2 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L7-B2 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pME-2PH-PLCd

Plasmid

#71283

Purpose

Multisite gateway entry clone for adding C-terminal fusions of human 2PH-PLC-delta

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2PH-PLC delta (Human)

Use

Gateway multisite middle entry clone

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pSGE_91_4xUAS_dCP

Plasmid

#71169

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

91_4xUAS_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pTYB3-human PUM1-HD PUF531_Q867E-Q939E-C935S-Q1011E- C1007S-intein-CBD

Plasmid

#73306

Purpose

Expresses mutant human PUM1-HD

Depositor

Traci Hall

Article

Wang et al Nat Methods. 2009 Nov;6(11):825-30. doi:

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Tags

intein

Expression

Bacterial

Mutation

Q867E-Q939E-C935S-Q1011E-C1007S

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pSGE_768_Nhe2-EcR_dCP

Plasmid

#71223

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

768_Nhe2-EcR_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFOSGE_CUS1A07

Plasmid

#71083

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

grn (grn Fly)

Use

Gateway Cloning

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pSGE_769_DipB-EcR_dCP

Plasmid

#71222

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

769_DipB-EcR_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pSGE_716_S2-1_GATA-to-DFD

Plasmid

#71173

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

716_S2-1_GATA-to-DFD

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pSGE_539_2xUAS_dCP

Plasmid

#71168

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

539_2xUAS_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pSGE_662_S2-1-Tor_dCP

Plasmid

#71165

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

662_S2-1-Tor_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pSGE_709_S2-1-Pal_dCP

Plasmid

#71162

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

709_S2-1-Pal_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1516

Plasmid

#62386

Purpose

pFA6a Osm1(M1A)-yoEGFP::LEU2

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

OSM1-EGFP fusion

Tags

EGFP

Expression

Yeast

Mutation

Methionine 1 to Alanine

Promoter

Adh1p

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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Xp75 DDD

Plasmid

#61282

Purpose

Expresses Xenopus p75 DDD domain

Depositor

Mark Bothwell, Lara Hutson

Article

Hutson et al J Neurobiol. 2001 Nov 5;49(2):79-98.

Insert

p75 DDD (p75NTRa Frog)

Use

Xenopus expression

Expression

Mammalian

Mutation

lacks the putative death domain (contains amino a…

Promoter

CMV

Available Since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only
pCS2 xenopus twisted gastrulation

Plasmid

#24974

Depositor

Edward De Robertis

Article

Larrain et al Development. 2001 Nov . 128(22):4439-47.

Insert

twisted gastrulation (Twsg1 Mouse)

Tags

FLAG and chordin signal peptide

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pFP121

Plasmid

#21670

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only