Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 8,392 results for: crispr grnas

Clear keywords
Showing: 6481 - 6500 of 8392 results

  2. mU6-sgLkb1_1st-hU6-sgNT

    Plasmid
    #177220
    Purpose
    Expresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgLkb1_1st/sgNT1
    Use
    Lentiviral
    Promoter
    mU6/hU6
    Available Since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177220-plasmid-map-sequence-id-350037

    Enlarge

  3. mU6-sgSik1_2nd-hU6-sgSik3_2nd

    Plasmid
    #177219
    Purpose
    Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_2nd/sgSik3_2nd
    Use
    Lentiviral
    Promoter
    mU6/hU6
    Available Since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177219-plasmid-map-sequence-id-350035

    Enlarge

  6. Direct-seq_lentiGuide-Puro-Tetra Loop-8A8G

    Plasmid
    #157982
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Article
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (Tetra-8A8G)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    157982-plasmid-map-sequence-id-332394

    Enlarge

  7. pCAG-nucGFP11-3xControlgRNA

    Plasmid
    #224568
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    GFP11-H2B
    Use
    CRISPR
    Tags
    Histone H2B (nuclear localization)
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224568-plasmid-map-sequence-id-451496

    Enlarge

  10. pKLV-gRNA-CTBP2-MGMT_1

    Plasmid
    #136419
    Purpose
    Lentiviral expression of gRNAs targeting intron 1 of human CTBP2 and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.
    Depositor
    Massimo Squatrito
    Insert
    U6_sgRNA(CTBP2)_U6_sgRNA(MGMT)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    136419-plasmid-map-sequence-id-271761

    Enlarge

  13. pDual_dsCas9_Venus_hs_NQO1

    Plasmid
    #214684
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human NQO1
    Depositor
    Kevin Janes
    Insert
    dgRNA_NQO1 (NQO1 Human)
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214684-plasmid-map-sequence-id-428771

    Enlarge

  14. pDual_dsCas9_Venus_hs_TP73

    Plasmid
    #214685
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214685-plasmid-map-sequence-id-428818

    Enlarge

  15. pDual_dsCas9_BFP_hs_TP73

    Plasmid
    #214687
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214687-plasmid-map-sequence-id-428806

    Enlarge

  17. pADH118

    Plasmid
    #90983
    Purpose
    NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. albicans LEU2 2 of 2
    Use
    CRISPR
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90983-plasmid-map-sequence-id-180511

    Enlarge

  18. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

    Plasmid
    #166693
    Purpose
    Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.
    Depositor
    Elvir Becirovic
    Article
    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi
    Inserts
    dCas9-VPR
    3x Cnga1 promoter-targeting sgRNAs
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    TRE and U6
    Available Since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    166693-plasmid-map-sequence-id-331043

    Enlarge

  20. AgU6.695_3xP3-DsRed1-SV40 (Single gRNA)

    Plasmid
    #243009
    Purpose
    For cloning a single gRNA after BspQI digestion under An. gambiae U6.695 promoter.
    Depositor
    George Christophides
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Insect
    Available Since
    Sept. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    243009-plasmid-map-sequence-id-486379

    Enlarge

Showing: 6481 - 6500 of 8392 results