We narrowed to 18,525 results for: puro
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BARBEKO sgRNA library
Pooled Library#174163PurposeCytosine base editors for genome-scale knockout screens in human cells. Targets gene start codons, splice sites, or introduces premature termination codons.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable SinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP- SCAT3.2
Plasmid#172497PurposeSCAT3.2 is fluorescent reporter (FRET probe) for cell survival/apoptosis with Caspase-3 sensitive sequence which can be introduced to mammalian cells by retrovirus vector.DepositorInsertAmcyan
UseRetroviralTagsmVenusExpressionMammalianPromoterMLV LTRAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
p8092 LentiCRISPR v2 sgNT-1
Plasmid#163315PurposeExpresses Cas9 and a non-targeting control guide RNADepositorInsertsgNT-1
UseCRISPR and LentiviralPromoterU6Available SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
dCas9-ddMSK1
Plasmid#165603PurposeExpresses Sp dCas9 fused to truncated inactive human MSK1 (42-802, D195A, D565A)DepositorInsertS. Pyogenes dCas9 with c-terminal truncated inactive human Mitogen- and stress-activated protein kinase-1 (42-802, D195A, D565A) (RPS6KA5 Synthetic, Human, S. Pyogenes)
UseCRISPR and LentiviralTagsFLAG TagExpressionMammalianPromoterEF1aAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMH-EBFP
Plasmid#161714PurposeGateway-compatible destination vector for mammalian expression of N-terminal EBFP protein under CMV promoterDepositorTypeEmpty backboneTagsEBFPExpressionMammalianPromotercmvAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEXPqcxip-hSURF4-FLAG
Plasmid#159139PurposeHu SURF4 -flag mammalian expression Gateway vector.DepositorInsertsurfeit 4 (SURF4 Human)
UseRetroviralTagsFLAGExpressionMammalianPromoterCMV (cytomegalovirus) enhancer and the MSV (mouse…Available SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRT LAMP3 mCRISPRed_mTagBFP2 pcDNA5
Plasmid#159455PurposeEncodes a lysosome tagret fusion protein of the pH resistant proteins mCRISPRed and mTagBFP2DepositorInsertmCRISPRed
TagsmTagBFP2ExpressionMammalianPromoterCMVAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-PDL1
Plasmid#159280PurposeHuman AAVS1 targeting vector for knockin of a CAGGS promoter-driven human PDL1/CD274 (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.DepositorInsertHuman PDL1/CD274 (CD274 Human)
UseAAV; S1 knockin donor vectorExpressionMammalianPromoterCAGGSAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-LMW-FGF2
Plasmid#157663PurposeExpresses FGF2 in mammalian cellDepositorInsertFibroblast growth factor 2 (FGF2 Human)
TagsHA tagExpressionMammalianMutationdeleted amino acid 1-134Available SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDA027
Plasmid#131385PurposeORFeus expression, mutantDepositorInsertORFeus
TagsORF2-3xFlagExpressionMammalianMutationH230A, D702YAvailable SinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4134903
Plasmid#151951PurposeMammalian expression plasmid for SARS-CoV-2 N (Nucleocapsid)DepositorInsertSARS-CoV-2 N (Nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
ExpressionMammalianMutationwtPromoterCMVAvailable SinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4134906
Plasmid#151960PurposeMammalian expression plasmid for Human coronavirus OC43 nucleocapsidDepositorInsertHuman coronavirus OC43 nucleocapsid protein (EYW02_gp8 Human coronavirus OC43)
ExpressionMammalianMutationwtPromoterCMVAvailable SinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAS_4xMma PylT_FLAG-AcKRS
Plasmid#140021PurposePlasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integrationDepositorInsertAcKRS-3 (pylS Methanosarcina mazei)
TagsFLAGExpressionMammalianMutationL301M, L305I, Y306F, L309A, C348FPromoterEF1Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Cep152
Plasmid#136825PurposeMammalian expression of the centrosomal protein Cep152 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep152 (CEP152 Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
px459-TDP-43 sgRNA1
Plasmid#133762PurposeExpresses Cas9 and human TDP-43 sgRNADepositorAvailable SinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-SI-112
Plasmid#131127PurposeC-to-T base editing reporterDepositorInsertMutant EGFP
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterCMV promoterAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-SI-121
Plasmid#131126PurposeA-to-G base editing reporterDepositorInsertmutant EGFP
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterCMV promoterAvailable SinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shcGAS-Mmu
Plasmid#127698PurposeDoxycyclin inducible shRNA knockdown of mouse cGAS geneDepositorAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-cGASΔN-HA
Plasmid#130912PurposeExpresses human cGASΔN (aa160-522)-HA; Puromycin selection markerDepositorInsertcGASΔN
TagsHAExpressionMammalianPromoterCMVAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only
