We narrowed to 7,705 results for: tev

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OR2M4_Deletion_gRNA

Plasmid

#195194

Purpose

dual gRNAs for deletion of OR2M4 in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

OR2M4 dual gRNA (OR2M4 Human)

Expression

Mammalian

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold)

Plasmid

#113041

Purpose

AAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

gRNA CCR5 (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-EMX1 gRNA (SpyCas9 scaffold)

Plasmid

#113040

Purpose

AAV vector; encodes GFP as well as a U6-driven EMX1-targeting gRNA (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

gRNA EMX1 (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1mLCAD_K42R_FLAG

Plasmid

#24486

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Lys42Arg

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold)

Plasmid

#113042

Purpose

AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

CFTR gRNA (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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ABE8.13-d

Plasmid

#136297

Purpose

expresses ABE8.13-d in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.13-d

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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TRBV16

Plasmid

#123451

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV16 (TRBV16 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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AcrIIC1X* (AcrIIC1X-mCherry chimera)

Plasmid

#128114

Purpose

Expresses AcrIIC1(N3F/D15Q/A48I) fused to mCherry; mediates potent inhibition of both, N. meningitidis as well as S. aureus Cas9

Depositor

Dominik Niopek

Article

Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

Insert

AcrX* (AcrIIC1 N3F/D15Q/A48I fused to mCherry)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5R 99xGly-uFMR1-3xHA

Plasmid

#242192

Purpose

Transient expression of 99xGly-uFMR1-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

99xGly-uFMR1-3xHA (FMR1 Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2026

Availability

Academic Institutions and Nonprofits only

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pRK5R 15xGly-uFMR1-3xHA

Plasmid

#242191

Purpose

Transient expression of 15xGly-uFMR1-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

15xGly-uFMR1-3xHA (FMR1 Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLJY1 DD-FAM98B[1-330]

Plasmid

#211353

Purpose

Constitutive lentiviral expression of DD-V5-FAM98B[1-330] fusion protein

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

DD-FAM98B[1-330] (FAM98B Human)

Use

Lentiviral

Tags

FKBP12 DD and V5

Mutation

Truncation of C-terminal glycine-rich IDR

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pYO379

Plasmid

#235733

Purpose

Protein expression of VPS34 + VPS15-ZZ + ZZ-VPS30 (all yeast) in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS34 (VPS34 Budding Yeast)
VPS15 (VPS15 Budding Yeast)
VPS30 (VPS30 Budding Yeast)

Tags

3xTEV-ZZ and ZZ-3xTEV

Expression

Yeast

Mutation

S2A on ScVPS34

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGM84

Plasmid

#220172

Purpose

CRISRPi guide RNA against BTF3

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

BTF3 (sgRNA) (BTF3 Human)

Use

Lentiviral

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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Chr1q_Cassette-Proximal_gRNA

Plasmid

#195127

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting location immediately downstream of Chr1q_cassette-integration_gRNA, to be used for cassette deletion controls

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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TRAV29

Plasmid

#123401

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV29 (TRAV29DV5 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMDC32-FIT2-N[80]A

Plasmid

#96992

Purpose

Express mouse FIT2 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

Insert

MmFIT2 (Fitm2 Mouse)

Expression

Plant

Mutation

Amino acid residue 80 was mutated (N[80]A). Mutat…

Promoter

35S

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1mLCAD_K42Q_FLAG

Plasmid

#24487

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Lys42Gln

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1mLCAD_K42A_FLAG

Plasmid

#24488

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Lys42Ala

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
COVID-SARS2 NSP14/10

Plasmid

#159613

Purpose

Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10

Depositor

Opher Gileadi

Article

Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650

Inserts

Covid-SARS2 Nsp14 (ORF1ab Synthetic, COVID-SARS2)
Covid-SARS2 Nsp10

Tags

His6, TEV cleavage site and none

Expression

Bacterial

Mutation

Codon-optimized for E. coli expression

Promoter

(Bicistronic) and T7 - LacO

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b-FNLDTA

Plasmid

#195340

Purpose

Expresses Engineered BrCas12b in Bacteria

Depositor

Piyush Jain

Article

Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis Tag and MBP, TEV cleavage site, 6x His Tag

Expression

Bacterial

Mutation

F208W, N524V, L795I, D868V, T874S, A1015E

Promoter

T7 Promoter

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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