We narrowed to 9,753 results for: try

1. CROPseq-Puro-4xMS2

   Plasmid

   #212626

   Purpose

   MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSIN-WP-mPGK-GDNF-IRES-tTA-KRAB

   Plasmid

   #225488

   Purpose

   Tet-on lentiviral vector for controlled expression of GDNF

   Depositor

   Robert Gross

   Article

   Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

   Insert

   Tetracycline-controlled transactivator fused with Krüppel-associated box (KRAB) domain following an internal ribosome entry site
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Mouse phosphoglycerate kinase 1

   Available Since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME rras_Q61R

   Plasmid

   #201057

   Purpose

   Gateway middle entry vector expressing constitutively actve zebrafish rras

   Depositor

   Sarah Childs

   Article

   Greysson-Wong et al Development. 2023 Sep 15;150(18):dev2018

   Insert

   RRas_Q61R (rras Zebrafish)
   

   Use

   Zebrafish expression

   Mutation

   Mutation Q61R, constitutively active

   Promoter

   none

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. MLKS2delARMec

   Plasmid

   #130860

   Purpose

   Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

   Depositor

   Hank Bass

   Article

   Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

   Insert

   MLKS2
   

   Use

   Gateway entry vector

   Tags

   eGFP-FLAG-HA

   Mutation

   Deletion of armadillo domain

   Available Since

   Sept. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. MLKS2del4ec

   Plasmid

   #130857

   Purpose

   Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

   Depositor

   Hank Bass

   Article

   Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

   Insert

   MLKS2
   

   Use

   Gateway entry vector

   Tags

   eGFP-FLAG-HA

   Mutation

   Deletion of terminal four amino acids

   Available Since

   Sept. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. MLKS2delKASHec

   Plasmid

   #130858

   Purpose

   Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

   Depositor

   Hank Bass

   Article

   Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

   Insert

   MLKS2
   

   Use

   Gateway entry vector

   Tags

   eGFP-FLAG-HA

   Mutation

   eGFP-FLAG-HA

   Available Since

   Sept. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pENTR-TC9

   Plasmid

   #202088

   Purpose

   entry vector for gateway recombination of TevCas9 and sgRNA into a destination cassette in pCitro-dest

   Depositor

   David Edgell

   Article

   Hamilton et al ACS Synth Biol. 2023 Dec 15;12(12):3578-

   Insert

   TevCas9 + sgRNA cassette
   

   Use

   CRISPR

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. eDrTrkB

   Plasmid

   #183997

   Purpose

   Optically controlled eDrTrkB expression in mammalian cells

   Depositor

   Dmitry Gerashchenko, Vladislav Verkhusha

   Article

   Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

   Insert

   eDrTrkB (NTRK2 Human)
   

   Tags

   Ig kappa secretory signal, mCherry, Deinococcus r…

   Expression

   Mammalian

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. KTK_346

   Plasmid

   #180549

   Purpose

   Entry vector containing gfasPurple

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   gfasPurple
   

   Expression

   Bacterial

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ265N2

    Plasmid

    #174001

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    rAPOBEC1-zCas9(D10A)-SpRY-UNG
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ265O2

    Plasmid

    #174002

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ265L2

    Plasmid

    #174000

    Purpose

    Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENT-L1-Kozak-NLSlacZ-stop-L2

    Plasmid

    #186358

    Purpose

    Entry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    lacZ (lacZ Synthetic, E. coli)
    

    Use

    Expression of nuclear lacz reporter

    Tags

    Nuclear localization signal

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMpGE_En03-sgRNA_Target2 (Mpphot)

    Plasmid

    #186727

    Purpose

    Gateway entry vector for sgRNA (target 2: Mpphot [negative control]). Transient expression of sgRNA (target 2: Mpphot) in plant cells.

    Depositor

    Yutaka Kodama

    Article

    Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

    Insert

    sgRNA_Mphot
    

    Expression

    Bacterial

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMpGE_En03-sgRNA_Target1 (Mpphot)

    Plasmid

    #186726

    Purpose

    Gateway entry vector for sgRNA (target 1: Mpphot [positive control]). Transient expression of sgRNA (target 1: Mpphot) in plant cells.

    Depositor

    Yutaka Kodama

    Article

    Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

    Insert

    sgRNA_Mphot
    

    Expression

    Bacterial

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR/Zeo-sfGFP-SKL

    Plasmid

    #186720

    Purpose

    Gateway entry vector encoding sfGFP with the two peroxisome-targeting signals (out of frame).

    Depositor

    Yutaka Kodama

    Article

    Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

    Insert

    sfGFP-SKL
    

    Expression

    Bacterial

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. KTK_316

    Plasmid

    #180547

    Purpose

    Entry vector containing sfGFP

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    sfGFP
    

    Expression

    Bacterial

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. KTK_344

    Plasmid

    #180548

    Purpose

    Entry vector containing cjBlue

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    cjBlue
    

    Expression

    Bacterial

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. KTK_345

    Plasmid

    #180550

    Purpose

    Entry vector containing eforRed

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    eforRed
    

    Expression

    Bacterial

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. MNEAP1ec

    Plasmid

    #131020

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

    Insert

    MNEAP1
    

    Use

    Gateway entry vector

    Tags

    eGFP-FLAG-HA

    Available Since

    May 6, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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