163,636 results

1. pdCas9-sgRNA-RFP

   Plasmid

   #166005

   Purpose

   Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

   Depositor

   Martin Buck

   Article

   Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP scFv [N86/20]

   Plasmid

   #206744

   Purpose

   Mammalian Expression of GFP scFV. Derived from hybridoma N86/20 scFv.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   GFP (Aequorea victoria) recombinant scFV
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti-mTagBFP2

   Plasmid

   #216126

   Purpose

   Lentiviral vector for expressing mTagBFP2.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-mTagBFP2-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL198

   Plasmid

   #180472

   Purpose

   For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

   Depositor

   Peggy Lemaux

   Article

   Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   ZmCas9

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 HA Arf6

   Plasmid

   #10834

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf6 (ARF6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. Active Wnt2-V5

   Plasmid

   #43809

   Depositor

   Xi He

   Article

   MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

   Insert

   WNT2 (WNT2 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-CMV-V5-RFP-SLMAP (isoform r)

   Plasmid

   #235685

   Purpose

   Express RFP tagged SLMAP gene in mammalian cells using the CMV promoter

   Depositor

   Duojia Pan

   Article

   Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

   Insert

   SLMAP (SLMAP Human)
   

   Use

   Lentiviral

   Tags

   V5-RFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA 3.1 KBTBD4 WT

   Plasmid

   #184627

   Purpose

   Mammalian expression vector with N-terminal 3xFlag for KBTBD4 WT expression in mammalian cells

   Depositor

   Vincenzo D'Angiolella

   Article

   Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

   Insert

   N-terminal 3xFlag tagged KBTBD4 WT (KBTBD4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   no mutation

   Promoter

   CMV

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

   Plasmid

   #219565

   Purpose

   3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome

   Depositor

   Andrea Ventura

   Article

   Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

   Insert

   HygroR_2A_H2B-GFP-N_loxP_mScarlet
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Construct 28 -hUBCp_TetR_p2a_LacI_p2a_ZeoR

    Plasmid

    #81253

    Purpose

    Lentiviral construct for building stable cell lines expressing TetR and LacI via Zeocin selection

    Depositor

    Timothy Lu

    Article

    Perli et al Science. 2016 Aug 18. pii: aag0511.

    Insert

    TetR and LacI
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCBC-MT1T2

    Plasmid

    #50593

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T1, gRNA scaffold, OsU3t plus, TaU3p, T2
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX-4T1-14-3-3 zeta GST

    Plasmid

    #13278

    Depositor

    Michael Yaffe

    Article

    Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

    Insert

    14-3-3 zeta (YWHAZ Bovine)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pDONR223-WNK1

    Plasmid

    #23670

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    WNK1 (WNK1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    G4C

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP

    Plasmid

    #226516

    Purpose

    Mamalian cell expression of SNAP-tag2 on mitochondrial surface and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    TOMM20-SNAP-tag2-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKL001

    Plasmid

    #98917

    Purpose

    Constitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Insert

    CaPR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23118 - Strong Constitutive

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-5X1 Flag PA1

    Plasmid

    #15560

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    PA1 (PAGR1 Human)
    

    Use

    Tags

    Flag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

17. LRP4 (extracellular) scFv [N207/27]

    Plasmid

    #190537

    Purpose

    Mammalian Expression of LRP4 (extracellular) scFV. Derived from hybridoma N207/27.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    LRP4 (extracellular) (Mus musculus) recombinant scFV (Lrp4 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NL4-3 mCherry Luciferase

    Plasmid

    #44965

    Purpose

    HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.

    Depositor

    Warner Greene

    Article

    Lassen et al PLoS One. 2012;7(1):e30176. doi: 10.1371

    Insert

    mCherry, Luciferase, IRES
    

    Use

    Lentiviral

    Tags

    Luciferase, IRES and mCherry with D212G

    Expression

    Mutation

    S34C mutation in Nef

    Promoter

    Available since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCh-KIF5B*-strep

    Plasmid

    #120164

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 5B (Kif5b Mouse)
    

    Use

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF5B: 1-555 aa

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti6/V5-DEST-FASCIN

    Plasmid

    #31207

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    FASCIN (FSCN1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge