We narrowed to 7,924 results for: mme
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Plasmid#162923PurposeGateway attB4/attB1r entry clone for mammalian expression using the SV40 promoterDepositorInsertSV40 immediate early promoter
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1-D1135E
Plasmid#154924PurposeaTc inducible Cas9HF1 with D1135E mutationDepositorInsertCas9HF1-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationN497A/R661A/Q695A/Q926A/D1135E and stop codon on …PromoterpTetAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEE22
Plasmid#161787PurposeHi-SC2 reporter delivery plasmidDepositorInsertHi-SC2 reporter
UseConjugative plasmid to deliver hi-sc2 reporterMutationHimar site included MmeI site, tandem loxP sites,…Promotern/aAvailable SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pISH-Olfr1395
Plasmid#136731PurposeTemplate to synthesize riboprobes for in situ hybridization (ISH) for Olfr1395DepositorInsertOlfr1395
UseIn situ probeMutationISH Probe insert, D175G and L220F- please dep. co…Available SinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-lambdaN-UPF1-DEAA
Plasmid#136007PurposeDEAA UPF1 inserted with lambdaN tagged on the N-terminus to use with the Tethering Assay (BoxB)DepositorInsertUPF1
TagsLambdaNExpressionMammalianMutationDE647AA and S743A (Please see depositor comments …Available SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMLS415
Plasmid#114272PurposeSapTrap donor vector for point mutagenesis; pENTR-221::syntron(lox2272-119+)DepositorInsertCbr-Unc-119
UseCRISPR and Cre/LoxMutation*please see depositor comment belowAvailable SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKSJ375
Plasmid#103064PurposeExpresses ImmE1, immunity to colicin E1DepositorExpressionBacterialPromoterE1 immunity protein promoter from ColE1Available SinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-Fv2E-flt4-ns
Plasmid#67695Purposemiddle entry plasmid with dimerizable extracellular domain fused to Danio Flt4 cytoplasmic domainDepositorInsertFv2E-flt4
Mutationno stop codon; D1302E (see depositor's comme…Available SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
4QL1
Plasmid#61942PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWDR5:APC041-G05:C36415 (WDR5 Human)
Tags6x His and TEV cleavage siteExpressionBacterialMutationSee commentsPromoterT7Available SinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
SpDJ-1 pET15b
Plasmid#60689PurposeRecombinant protein expression of DJ-1DepositorAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
4MI0
Plasmid#60097PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertEZH2:JMC033-A03:C216172 (EZH2 Human)
UseBaculovirus expressionTags6xHisMutationSee commentsPromoterT7Available SinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
FLAG-Sec31a W985A #790
Plasmid#42122DepositorInsertSec31a (SEC31A Human)
TagsFLAGExpressionMammalianMutationG742R and W985A NOTE: the mutation is actually W…PromoterCMVAvailable SinceMarch 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
XE5 Xwnt-5A Hind RNA
Plasmid#16872DepositorInsertXwnt-5A Hind RNA (wnt5a.S Frog)
UseXenopus expressionMutationStarts with AA 14 (see comments)Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMT-Pygopus
Plasmid#31900DepositorAvailable SinceSept. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMT FRB-Draper INT-GFP
Plasmid#126665PurposeInducible expression of a synthetic FRB-Draper receptor in S2 cellsDepositorInsertFRB-Draper INT domain
ExpressionInsectMutationK986N- see depositor comments belowAvailabilityAcademic Institutions and Nonprofits only -
pET28a SnoopTag-mEGFP-SpyTag
Plasmid#72325PurposeBacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.DepositorInsertpET28a SnoopTag-mEGFP-SpyTag
TagsN-terminal His6 tagExpressionBacterialAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-taCasp3-T2A-TEVp-WPRE3-BGHpA
Plasmid#242456PurposeAAV plasmid for expressing taCaspase-3 and T2A linked TEVp for caspase mediated cell ablation, and an intersectional strategy for Cre-off and Flp-on expression. See comments for more detail.DepositorAvailable SinceDec. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
APEX2-OMM
Plasmid#79056PurposeSoybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling proteinDepositorInsertSoybean APEX2
UseLentiviralTagsflag and last 31 amino acids of human MAVSExpressionBacterial and MammalianPromoterCMV immearly promotorAvailable SinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
ERM-APEX2
Plasmid#79055PurposeSoybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.DepositorInsertAPEX2 anchored to the cytosolic face of the ER membrane
UseLentiviralTagsResidues 1-27 of P450 oxidase 2C1 and V5ExpressionBacterial and MammalianPromoterCMV immearly promotorAvailable SinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLSC-5
Plasmid#62889PurposeLenti vector for expression of inducible split Cas9DepositorInsertsSpCas9(574-1368)
SpCas9(2-573)
UseCRISPR and LentiviralTagsFFBP12, FRB, NES PTK2, NLS SV40, and P2AExpressionMammalianMutation*see comment belowPromoterEFSAvailable SinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only