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SK-YFP-NM-A

Plasmid

#48664

Purpose

Bacterial NM repression YFP reporter: protospacer A

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

NM prototspacer A/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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SK-YFP-TD-A

Plasmid

#48666

Purpose

Bacterial TD repression YFP reporter: protospacer A

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TD prototspacer A/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pDL128

Plasmid

#43912

Depositor

Stephen Elledge

Article

Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

Insert

DIF1 (DIF1 Budding Yeast)

Expression

Yeast

Mutation

SML domain mutated PPMLINQRT->AAAAAAAA, S59G, …

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pDL127

Plasmid

#43911

Depositor

Stephen Elledge

Article

Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

Insert

DIF1 (DIF1 Budding Yeast)

Expression

Yeast

Mutation

HUG domain mutated VGMRIRQ->AAAAAAA, S59, Q66H

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pJB052

Plasmid

#22620

Depositor

Lynn Hudson

Article

Berndt et al J Biol Chem. 1992 Jul 25. 267(21):14730-

Insert

Human genomic PLP promoter region (PLP1 Human)

Use

Cat reporter plasmid

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pJB035

Plasmid

#22622

Depositor

Lynn Hudson

Article

Berndt et al J Biol Chem. 1992 Jul 25. 267(21):14730-

Insert

human genomic PLP promoter region (POU3F2 Human)

Use

Cat reporter plasmid

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pJB038

Plasmid

#22625

Depositor

Lynn Hudson

Article

Berndt et al J Biol Chem. 1992 Jul 25. 267(21):14730-

Insert

human genomic PLP promoter region (POU3F2 Human)

Use

Cat reporter plasmid

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pJB048

Plasmid

#22641

Depositor

Lynn Hudson

Article

Berndt et al J Biol Chem. 1992 Jul 25. 267(21):14730-

Insert

human genomic PLP promoter region (POU3F2 Human)

Use

Cat reporter plasmid

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pSP64TS Activin pro Vg1 Flag (DM#171)

Plasmid

#15010

Depositor

Douglas Melton

Article

Reilly et al Cell. 1996 Sep 6. 86(5):743-54.

Insert

activin-Vg1

Use

Sp6 expression for use in xenopus

Tags

Flag / HMK

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
RCAS (A) Flu cTbx4 (CT#700)

Plasmid

#13925

Depositor

Cliff Tabin

Article

Logan et al Development. 1998 Aug . 125(15):2825-35.

Insert

Tbx4 (TBX4 Chicken)

Use

Retroviral; Avian expression

Tags

Flu (3xHA)

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pAM003

Plasmid

#170007

Purpose

Encodes PmgtC-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

Superfolder GFP
PhoP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLTetO-1 and PmgtC

Availability

Academic Institutions and Nonprofits only

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pAM004

Plasmid

#170008

Purpose

Encodes PugtL-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

Superfolder GFP
PhoP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLTetO-1 and PugtL

Availability

Academic Institutions and Nonprofits only

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pAM006

Plasmid

#170010

Purpose

Encodes PpagC-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

Superfolder GFP
PhoP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLTetO-1 and PpagC

Availability

Academic Institutions and Nonprofits only

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pKB234

Plasmid

#170021

Purpose

Encodes LacI-controlled expression of surface-displayed hBD3 and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
hBD3

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(del6) A259C

Plasmid

#111329

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

branch site A259C

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(del6) C256A

Plasmid

#111331

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

branch site C256A

Availability

Academic Institutions and Nonprofits only

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pAAV-DJ-N589X

Plasmid

#130878

Purpose

Encodes the AAV-DJ capsid with the N589 residue replaced with a TAG stop codon

Depositor

Prashant Mali

Article

Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

Insert

AAV-DJ cap N589X

Use

AAV

Expression

Mammalian

Mutation

N589X

Promoter

Unknown

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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DS-SPcas

Plasmid

#48645

Purpose

Bacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycin

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
tracrRNA precursor

Use

CRISPR

Promoter

proC and tracrRNA promoter

Available Since

Dec. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pBiFC-VC155

Plasmid

#22011

Purpose

Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

VC155

Tags

HA

Expression

Mammalian

Mutation

Changed Alanine 206 to Lysine

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pBiFC-VN173

Plasmid

#22010

Purpose

Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

VN173

Tags

FLAG

Expression

Mammalian

Mutation

Venus residus 1-172

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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