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pC1300_pUB10_pcoCASphi_E9t_MCS_version1

Plasmid

#197937

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pcoCasphi-2

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr-CM

Plasmid

#188585

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler Catalytic mutant

Tags

FLAG-Myc

Expression

Insect

Mutation

deleted first ATG; D435A [GAT=>GCC]; E435A [GA…

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[N1-383]

Plasmid

#188586

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler N-terminal domain

Tags

FLAG-Myc

Expression

Insect

Mutation

deleted first ATG

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[C399-625]

Plasmid

#188587

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler C-terminal domain

Tags

FLAG-Myc

Expression

Insect

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pRB121

Plasmid

#181945

Purpose

aTc-inducible expression of dimerized mNeonGreen (mNG-mNG)

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

mNeonGreen-mNeonGreen; tetR (AX04_RS23485 Synthetic)

Expression

Bacterial

Mutation

See Depositor Comments Below

Promoter

mNG-mNG-PLtetO-1; tetR-J23106

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pT-GFP-rG1

Plasmid

#188967

Purpose

IPTG inducible GFP with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

GFP
sgRNA: agtggaaaacaatgcgaccgactagt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pT-GFP-rG2

Plasmid

#188968

Purpose

IPTG inducible GFP with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

GFP
sgRNA: agtccatgtaatcagcgtctactagt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1_1-327-dsRNAres_H

Plasmid

#146501

Purpose

Insect Expression of DmDCP1_1-327

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_1-327 (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-dsRNAres_H

Plasmid

#146502

Purpose

Insect Expression of DmDCP1

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1 (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmDCP1_328-366 (MCSII)_G

Plasmid

#146402

Purpose

Bacterial Expression of DmDCP1_328-366

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_328-366 (Dcp1 Fly)

Expression

Bacterial

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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