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HUW-TetO-hNanog

Plasmid

#25700

Depositor

Rudolf Jaenisch

Article

Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

Insert

Nanog (NANOG Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt4

Plasmid

#35910

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt4 (WNT4 Human)

Expression

Mammalian

Mutation

Silent Mutation C (492) --> T

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt10

Plasmid

#184511

Purpose

Mammalian expression of Synaptotagmin 10 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 10 (Syt10 Mouse)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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Puro-iDEST

Plasmid

#75336

Purpose

GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

TRE-DEST

Expression

Mammalian

Promoter

TRE-tight

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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SLC30A10-GFP

Plasmid

#104381

Purpose

Expresses SLC30A10-GFP wild-type in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Selyunin et al J Cell Biol. 2017 Oct 2;216(10):3249-326

Insert

SLC30A10 (SLC30A10 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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NylB'_pET21b

Plasmid

#215416

Purpose

Expression construct for NylB' gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylB'

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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mini-donor - NKX2.5-nEGFP

Plasmid

#209287

Purpose

this mini-donor knockin vector can be used to knock nuclear EGFP into NKX2.5 locus

Depositor

Xiaojun Lian

Article

Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

Insert

EGFP-nls

Use

Knockin donor vector

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCK015

Plasmid

#105347

Purpose

Bacterial translocation fo proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Mutation

BsaI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pDctp-mSox10-Mp1

Plasmid

#13513

Depositor

William Pavan

Article

Hakami et al Mech Dev. 2006 Feb . 123(2):124-34.

Insert

Sox10 (Sox10 Mouse)

Tags

Dct promoter and Mp1-polyA

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only

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pLBM0010

Plasmid

#172816

Purpose

Level 2 module containing HSP21-GFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-GFP]

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab32b

Plasmid

#140885

Purpose

Expression of zebrafish GFP-tagged Rab32b in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab32b (rab32b Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCK013

Plasmid

#105345

Purpose

Bacterial translocation fo proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Mutation

BsaI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCK016

Plasmid

#105348

Purpose

Bacterial translocation for proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Mutation

BsaI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCK014

Plasmid

#105346

Purpose

Bacterial translocation fo proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Mutation

BsaI restriction sites removed

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-TTF-1-wt

Plasmid

#74990

Purpose

Retroviral vector holding the human wild-type full-length TTF-1 (NKX2-1) cDNA

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

TTF-1 (TTF1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

wild-type, full-length, without 3' UTR

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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HIR Y1158,1162,1163F

Plasmid

#24054

Depositor

Frederick Stanley

Article

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Expression

Mammalian

Mutation

Y1158F, Y1162F, Y1163F

Available Since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only
pOCC154

Plasmid

#118902

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette

Tags

TwinStrep, cleavable with 3C protease

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pME-nCas9n-P2A-TagBFP2-pA

Plasmid

#176026

Purpose

Multisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENT

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

nCas9n-P2A-TagBFP2

Use

Gateway entry clone

Available Since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 C506S

Plasmid

#78584

Purpose

Expression flag-USP19 C506S in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 C506S (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

mutate 506 cysteine residue to serine

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pME-APEX2-NS

Plasmid

#67669

Purpose

Gateway entry clone containing APEX2 with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2

Use

Gateway multisite middle entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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