We narrowed to 11,947 results for: emb
-
Plasmid#184914PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHRSin-IRES2-EmGFP_HPC-4 Ab heavy-6xHIS
Plasmid#187358PurposeLentiviral expression of HPC-4 antibody heavy chain, and 6xHIS tagDepositorInsertHPC-4 antibody heavy chain, and 6xHIS tag
UseLentiviralPromoterSFFVAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H
Plasmid#187186PurposeProtein ExpressionDepositorAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAW2-Smed-Slit-1
Plasmid#182264PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea Slit-1DepositorInsertSmed-Slit-1
UseGibson cloning vector for synthesis of single and…Available sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP1B 1-199
Plasmid#115326PurposeExpresses residues 1-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-24DepositorAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem161a-V5
Plasmid#175149PurposeLentiviral expression of mouse Tmem161a-V5DepositorAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem53-V5
Plasmid#175142PurposeLentiviral expression of mouse Tmem53-V5DepositorAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj14
Plasmid#173144PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj14 (kcnj14 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn1a
Plasmid#164956PurposeZebrafish kcnn1a gene CDSDepositorInsertkcnn1a (kcnn1a Zebrafish)
UsePcr cloning vectorAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r
Plasmid#162967PurposeGateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteinsDepositorInsertDual Strep2 purification tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E23: attB5r-RU5u-3xFLAG-nostop-attB1r
Plasmid#162958PurposeGateway attB5r/attB1r entry clone for generation of N-terminal 3xFLAG-tagged fusion proteinsDepositorInserttriple FLAG epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AtUBQ10 (GB2478)
Plasmid#160594PurposeUBQ10 promoter from A. thaliana for constitutive expression in plant systems.DepositorInsertAtUBQ10
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 dCas9 (GB2664)
Plasmid#160596PurposeCDS of dCas9 with B3 nomenclatureDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn2
Plasmid#164958PurposeZebrafish kcnn2 gene CDSDepositorInsertkcnn2 (kcnn2 Zebrafish)
UsePcr cloning vectorAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn3
Plasmid#164959PurposeZebrafish kcnn3 gene CDSDepositorInsertkcnn3 (kcnn3 Zebrafish)
UsePcr cloning vectorAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn4
Plasmid#164960PurposeZebrafish kcnn4 gene CDSDepositorInsertkcnn4 (kcnn4 Zebrafish)
UsePcr cloning vectorAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 MS2 (GB2665)
Plasmid#160597PurposeCDS of MS2 protein with B3 nomenclatureDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMS2
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
NDV-HN-SpyTag
Plasmid#160000PurposeExpresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cellsDepositorInsertNDV-HN-SpyTag
TagsHis6-tagExpressionMammalianPromoterChicken beta-actin promoterAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Eif4a1-L
Plasmid#122346PurposeExpresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA for mouse Eif4a1
ExpressionMammalianAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTV-mHdac1-tdiRFP670-PEST2
Plasmid#122351PurposeTargeting vector for mouse Hdac1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert2A-tdiRFP-PEST with homology arms for Hdac1
UseMouse TargetingAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only