We narrowed to 14,722 results for: EGF

1. pAS3-lim-4P_gtACR2_SL2_eGFP(65C)_unc-54 3'UTR

   Plasmid

   #195853

   Purpose

   Expresses inhibitory opsin gtACR2 and GFP in turning associated neurons

   Depositor

   Andrew Leifer

   Article

   Kumar et al PLoS Biol. 2023 Sep 21;21(9):e3002280. d

   Insert

   Guillardia theta anion-conducting channelrhodopsin-2 (GtACR2)
   

   Expression

   Worm

   Promoter

   lim-4

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin

   Plasmid

   #134860

   Purpose

   Expresses EGFP-tagged recycling endosome marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   RAB11B (RAB11B Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161)

   Plasmid

   #139992

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   VRQR=D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

   Plasmid

   #87828

   Purpose

   High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.

   Depositor

   Andrea Musacchio

   Article

   Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

   Inserts

   NLS
   PTS1
   

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Promoter

   CMV promoter, tetracycline operator

   Available Since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin

   Plasmid

   #133028

   Purpose

   Expresses EGFP-tagged Golgi apparatus marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Golgin84 (GOLGA5 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-673

   Promoter

   Human elongation factor 1 alpha

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

   Plasmid

   #139996

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-EGFP-P2A-iCre (JDW 921)

   Plasmid

   #224519

   Purpose

   A Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EGFP-P2A-iCre
   

   Use

   Cre/Lox

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

   Plasmid

   #99248

   Purpose

   single-vector system with EGFP reporter for use in CROPseq knockout screens

   Depositor

   Alex Hewitt

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP

   Plasmid

   #175521

   Purpose

   Nuclear expression of HaloTag10 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag10
   

   Expression

   Mammalian

   Mutation

   HaloTag10 = HaloTag7-Q165W

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

    Plasmid

    #134661

    Purpose

    Expresses EGFP-tagged synaptic vesicle protein

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    vesicle-associated membrane protein 2 (VAMP2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FLEX-hKCNQ3(R230C)-P2A-EGFP

    Plasmid

    #209194

    Purpose

    Cre-dependent expression of Kcnq3 with R230C mutation and GFP from the same transcript

    Depositor

    Larry Zweifel

    Article

    Gamal El-Din et al Proc Natl Acad Sci U S A. 2021 Nov 9;118

    Insert

    Kcnq3 (KCNQ3 Human)
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R230 to C230

    Promoter

    CMV

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJRH-1364 LV EF1a-CD4 IRES-EGFP

    Plasmid

    #201920

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD4

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD4 (CD4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

    Plasmid

    #175522

    Purpose

    Nuclear expression of HaloTag11 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag11
    

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. EK0441 CMV-TO-BFPmut-stop-EGFP.t2 (FLP-IN)

    Plasmid

    #191161

    Purpose

    Expression of a trigger sequence from EGFP CDS in 3' UTR context in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    EGFP.t2
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)

    Plasmid

    #127392

    Purpose

    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)

    Depositor

    Ryohei Yasuda

    Article

    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    changed Threonine 286 to Aspartic acid and Threon…

    Promoter

    pCAG

    Available Since

    July 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV CAMKII PSAM4 GlyR IRES EGFP

    Plasmid

    #119744

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    CamKII

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)

    Plasmid

    #136627

    Purpose

    Plasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-LAMA-R59
    

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

    Plasmid

    #223125

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-pCAG eGFP T2A SP HALO HAPLN1

    Plasmid

    #228200

    Purpose

    AAV vector expressing GFP and HaloTag-HAPLN1 under constitutive promoter

    Depositor

    Sungjin Park

    Article

    Sterin et al J Neurosci. 2024 Sep 9:e0666242024. doi:

    Insert

    HAPLN1 (Hapln1 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    constitutive CAG promoter

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. CaMKIIP-mEGFP-P2A-paAIP2 (R5AR6A)/pAAV

    Plasmid

    #91719

    Purpose

    AAV-mediated expression of mEGFP and paAIP2 (R5AR6A), the functional mutant of paAIP2

    Depositor

    Ryohei Yasuda

    Article

    Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

    Insert

    mEGFP-P2A-paAIP2(R5AR6A)
    

    Use

    AAV

    Promoter

    CaMKII 1.3

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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