We narrowed to 7,359 results for: GFP expression plasmids

1. PX458-3xHA-en31FnCas9

   Plasmid

   #201949

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en31FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   G1243T, E1369R, E1603H on FnCas9

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX458-3xHA-en1FnCas9

   Plasmid

   #201947

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en1FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   E1369R on FnCas9

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX458-3xHA-en15FnCas9

   Plasmid

   #201948

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en15FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   E1603H on FnCas9

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX458-3xHA-FnCas9ABEmax8.17d

   Plasmid

   #201955

   Purpose

   Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D11A on FnCas9, V82S, V106W, Q154R on mutant TadA

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]

   Plasmid

   #190236

   Purpose

   AAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinase

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Insert

   EGFP
   

   Use

   AAV

   Tags

   F2A, LDLR C-terminal sequence, mRFP1, myristoylat…

   Expression

   Mammalian

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. eA3A-BE3 (pRZ206)

   Plasmid

   #131315

   Purpose

   CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCX420

   Plasmid

   #229211

   Purpose

   Cytoplasmic-targeting Csm complex plasmid

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   Csm1; Csm2; Csm3(RNase mut)-2xsfGFP; Csm4; Csm5; Cas6
   

   Expression

   Mammalian

   Available Since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. KW57_HART_RAC1

   Plasmid

   #207399

   Purpose

   Lentiviral plasmid containing DDX3X reporter HART for RAC1 5UTR

   Depositor

   Stephen Floor

   Article

   Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

   Insert

   eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJBL7030

   Plasmid

   #133872

   Purpose

   Atz reporter plasmid

   Depositor

   Julius Lucks

   Article

   Silverman et al ACS Synth Biol. 2020 Mar 20;9(3):671-677

   Insert

   AtzO-sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   modification of pAtzDEF

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCH101

    Plasmid

    #72308

    Purpose

    Prhl/lac-w reporter plasmid

    Depositor

    Matthew Bennett

    Article

    Chen et al Science. 2015 Aug 28;349(6251):986-9. do

    Insert

    modified YFP
    

    Expression

    Bacterial

    Mutation

    T203Y sfGFP->YFP

    Promoter

    Prhl/lac-w

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNoROS-CAT

    Plasmid

    #170152

    Purpose

    Mammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidation

    Depositor

    Dimitri Pestov

    Article

    Sapio et al Front Mol Biosci. 2021 Apr 26;8:678488.

    Inserts

    catalase (Cat Mouse)
    roGFP2
    

    Use

    Genome integration via sleeping beauty transposon…

    Tags

    FLAG tag and Nucleolar Localization Sequence (NoL…

    Expression

    Mammalian

    Promoter

    RPBSA and TRE promoter

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Target-AID (pRZ762)

    Plasmid

    #131300

    Purpose

    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCBh-T7-SpG-P2A-mTagBFP2(RAS3583)

    Plasmid

    #223110

    Purpose

    pCBh Human expression plasmid for SpG with with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpG-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Spike Display_Part 1-5 DO

    Plasmid

    #172726

    Purpose

    Encodes a sfGFP dropout expression cassette in place of Parts 1-5 to be used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Syn-FLEX-ExSYTE

    Plasmid

    #216263

    Purpose

    Cre-dependent expressoin of ExSYTE (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoter

    Depositor

    Susumu Tomita

    Article

    Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

    Insert

    ExSYTE P2A EGFP - Rat DTE
    

    Use

    AAV

    Available Since

    May 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid

    #170977

    Purpose

    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.

    Depositor

    Miki Ebisuya

    Article

    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

    Insert

    CShroom3 (Shroom3 Mouse)
    

    Use

    Gateway cloning

    Tags

    SspB and mCherry

    Mutation

    Isoform X2, deleted aminoacids 1 - 1388

    Available Since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. FNL Combinatorial Cloning Platform

    Plasmid Kit

    #1000000211

    Purpose

    A collection of Multisite Gateway Entry clone plasmids which can be used in a combinatorial fashion to generate large numbers of tagged expression clones for protein expression in a variety of systems

    Depositor

    Dominic Esposito

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

18. Spike Display_Part 2 DO

    Plasmid

    #172722

    Purpose

    Encodes a sfGFP dropout expression cassette in place of Part 2 of HexaPro-D614G spike to be used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Spike Display_Part 1 DO

    Plasmid

    #172721

    Purpose

    Encodes a sfGFP dropout expression cassette in place of Part 1 of HexaPro-D614G spike to be used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Spike Display_Part-5 DO

    Plasmid

    #172725

    Purpose

    Encodes a sfGFP dropout expression cassette in place of Part 5 of HexaPro-D614G spike to be used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge