We narrowed to 18,589 results for: MUT

1. R4pGWB601_UBQ10p-miniTurbo-NES-YFP

   Plasmid

   #127369

   Purpose

   Binary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plants

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Tags

   GS linker, NES, V5, and YFP

   Expression

   Plant

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Arabidopsis UBQ10 promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWZL Blast Twist ER

   Plasmid

   #18799

   Depositor

   Bob Weinberg

   Article

   Mani et al Cell. 2008 May 16. 133(4):704-15.

   Insert

   twist (Twist1 Mouse)
   

   Use

   Retroviral

   Tags

   ER hormone binding domain

   Expression

   Mammalian

   Mutation

   G525R mutation in the ER. The presence of the G52…

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. human gamma-catenin pcDNA3

   Plasmid

   #16827

   Depositor

   Eric Fearon

   Article

   Caca et al Cell Growth Differ. 1999 Jun . 10(6):369

   Insert

   gamma-catenin (JUP Human)
   

   Expression

   Mammalian

   Available Since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

   Plasmid

   #127353

   Purpose

   gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   TurboID (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and mVenus

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-ATF6-(S1P-)

   Plasmid

   #32956

   Purpose

   For visualization of ATF6 in mammalian cells with a mutation in ATF6 that blocks cleavage by protease S1P. Activated protein will remain in the Golgi.

   Depositor

   Ron Prywes

   Article

   Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

   Insert

   ATF6 (ATF6 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S1Protease site mutation: R415A/R416A

   Promoter

   CMV

   Available Since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMinDis.X513-iNicSnFR3a-(CC93)-ER

   Plasmid

   #125121

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

   Depositor

   Henry Lester

   Article

   Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

   Insert

   iNicSnFR3a-(CC93)-ER
   

   Tags

   ER retention motif

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19_CoV-2_linker

   Plasmid

   #211731

   Purpose

   Linker fragment for direct rescue of SARS-CoV-2, including 5' Promoter and 3' Termination signals and 100bp SARS-CoV-2 homology regions

   Depositor

   Thomas Klimkait

   Article

   Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

   Insert

   Linker fragment SARS-CoV-2
   

   Expression

   Mammalian

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. UAS-FRT-GFP-FRT-u(M)Cas9

   Plasmid

   #127388

   Purpose

   Cas9 inducible by removal of a GFP flip-out cassette

   Depositor

   Michael Boutros

   Article

   Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

   Insert

   FRT-GFP-FRT-uORF(M)Cas9
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-GABRA1(A322D)-IRES2-EGFP

   Plasmid

   #170821

   Purpose

   human GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFP

   Depositor

   Tingwei Mu

   Article

   Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

   Insert

   Human GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)
   

   Tags

   IRES2-EGFP

   Expression

   Mammalian

   Mutation

   change Alanine 322 to Aspartate

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB1163

    Plasmid

    #210032

    Purpose

    Contains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blast

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas11-P2A-Cas6-T2A-Cas3
    rtta-T2A-blast
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-FLAG-CCR5

    Plasmid

    #98949

    Purpose

    Mammalian expression plasmid for N-terminal FLAG-tagged human CCR5

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CCR5 (CCR5 Human)
    

    Tags

    FLAG (dykdddd) epitope tag and Signal/leader sequ…

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. SOX9-T2A-H2B-EGFP repair template

    Plasmid

    #167972

    Purpose

    SOX9 reporter repair template

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    SRY-Box Transcription Factor 9 (SOX9 Human)
    

    Use

    CRISPR; Donor template

    Tags

    H2B-EGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. LUC01 (L/Sec/P)

    Plasmid

    #112144

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
    

    Expression

    Mammalian

    Mutation

    Cysteine 258 mutated to selenocysteine (U)

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCKC014

    Plasmid

    #226623

    Purpose

    pLenti wt hTdT template for library 3

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Insert

    Terminal deoxynucleotidyl transferase (DNTT Human)
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

    Plasmid

    #127356

    Purpose

    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and mVenus

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

    Plasmid

    #127355

    Purpose

    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and mVenus

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-BAF_L58R

    Plasmid

    #101776

    Purpose

    Expresses EGFP tagged mutant BAF (L58R) in human cells

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L58R mutation

    Promoter

    EF1a

    Available Since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pInducer20 CARD8-HA PAM E274R

    Plasmid

    #169984

    Purpose

    Tet/Dox-inducible mammalian expression of CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PAM, E274R

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC-FLAG-ZCD2

    Plasmid

    #79274

    Purpose

    Mammalian expression of ZCD2 with a C-terminal FLAG tag

    Depositor

    Rita Shiang

    Article

    Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

    Insert

    ZCD2 (CISD2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pCPH2A.X_S139A-GS3

    Plasmid

    #204749

    Purpose

    Expression of Cas9 and human H2AX with the S139A mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX S139A (H2AX Human)
    

    Expression

    Mammalian

    Mutation

    H2AX with the S139A mutation

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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