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pMJA008

Plasmid

#128512

Purpose

Expresses Xylanase C

Depositor

Marcos Alcocer

Article

Furniss et al Biochim Biophys Acta. 2002 Jul 29;1598(1

Insert

Xylanase C

Expression

Bacterial

Promoter

Histone 4B

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pNIC28-BpWcbI

Plasmid

#125591

Purpose

Overexpression plasmid for production of WcbI from Burkholderia pseudomallei

Depositor

Nicholas Harmer

Article

Vivoli et al IUCrJ. 2013 Oct 18;1(Pt 1):28-38. doi: 1

Insert

BpWcbI (wcbI Burkholderia pseudomallei)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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relAE335Q_NS1

Plasmid

#100917

Purpose

expresses E.coli relAE335Q from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

RelA fragment from E.coli with point mutation E335Q

Use

Cyanobacteria cloning vector

Tags

FLAG

Mutation

E 335 converted to Q

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMST-712

Plasmid

#118080

Purpose

pmbfA::cexA::ttrpC

Depositor

Michael Sauer

Article

Steiger et al Metab Eng. 2019 Mar;52:224-231. doi: 10.

Insert

CexA

Promoter

pmbfA

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJet1.2 ftsY

Plasmid

#117130

Purpose

FtsY gene cassette

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

ftsY

Use

Golden gate donor vector for gene targeting in ba…

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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rpaA_NS1

Plasmid

#101829

Purpose

drives expression of rpaA from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with its native promoter

Use

Cyanobacteria cloning vector

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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P0050::rpaA

Plasmid

#101830

Purpose

drives expression of rpaA from an rpaA-independent promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with p0050 promoter (+ gentamicin resistance casette)

Use

Cloning vector

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::galP_NS1

Plasmid

#101843

Purpose

drives expression of galP transporter from NS1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Ptrc promoter driving GalP transporter expression

Use

Cloning vector

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pPICZC_CeTRIC-B2_Del

Plasmid

#87345

Purpose

Inducible expression of CeTRIC-B2 in P.Pichia strains

Depositor

Zhenfeng Liu

Article

Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

Insert

TRIC-B2

Tags

polyhistidine

Expression

Yeast

Mutation

Δ253-313

Promoter

AOX1

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAWp7-5

Plasmid

#72737

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-[miR-128 + miR-132]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-CMVp-GFP-[miR-128 + miR-132]

Use

Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-18

Plasmid

#72750

Purpose

pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-132]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-132]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-17

Plasmid

#72749

Purpose

pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-132]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-132]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-16

Plasmid

#72748

Purpose

pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-132]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-132]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-15

Plasmid

#72747

Purpose

pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-128]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-128]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-14

Plasmid

#72746

Purpose

pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-13

Plasmid

#72745

Purpose

pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-128]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-128]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-11

Plasmid

#72743

Purpose

pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-124]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-124]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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c7-87/199_HLL

Plasmid

#63122

Purpose

Mm-cpn cysteine double mutant for crosslinking

Depositor

Tanja Kortemme

Article

Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

Insert

Mm-cpn c7_87/199_HLL (NEWENTRY )

Tags

6x HIS-tag

Expression

Bacterial

Mutation

C140S-C237S-C286A-C359T-C393A-C470S-C484S-K87C-S1…

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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c7-132/202_HLL

Plasmid

#63123

Purpose

Mm-cpn double cysteine mutant for crosslinking

Depositor

Tanja Kortemme

Article

Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

Insert

Mm-cpn c7_132/202_HLL (NEWENTRY )

Tags

6x HIS-tag

Expression

Bacterial

Mutation

C140S-C237S-C286A-C359T-C393A-C470S-C484S-Q132C-S…

Available Since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Myotilin-C-14

Plasmid

#55316

Purpose

Localization: Cytoskeleton, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Myotilin (MYOT Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits