We narrowed to 7,842 results for: trac
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Plasmid#157615PurposeMammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only
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pD649-HAsp-PAPLN(Trunc1)-COMP5AP-AviTag-9xHis
Plasmid#157603PurposeMammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-CEACAM8-COMP5AP-AviTag-9xHis
Plasmid#157294PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-NFAM1-COMP5AP-AviTag-9xHis
Plasmid#157066PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-His10-BTLAicd (AA190-289, Y243F, Y257F, Y282F)-TwinStrep
Plasmid#180806PurposeExpression of 10*His tagged human BTLA intracellular domain (AA190-289, Y243F, Y257F, Y282F)-TwinStrepDepositorInsertBTLAint (AA190-289, Y243F, Y257F, Y282F)-TwinStrep (BTLA Human)
ExpressionBacterialMutationBTLA (Y243F, Y257F, Y282F)PromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His10-BTLAicd (AA190-289, Y226F, Y257F, Y282F)-TwinStrep
Plasmid#180807PurposeExpression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y257F, Y282F)-TwinStrepDepositorInsertBTLAint (AA190-289, Y226F, Y257F, Y282F)-TwinStrep (BTLA Human)
ExpressionBacterialMutationBTLA (Y226F, Y257F, Y282F)PromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y282F)-TwinStrep
Plasmid#180808PurposeExpression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y282F)-TwinStrepDepositorInsertBTLAint (AA190-289, Y226F, Y243F, Y282F)-TwinStrep (BTLA Human)
ExpressionBacterialMutationBTLA (Y226F, Y243F, Y282F)PromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y257F)-TwinStrep
Plasmid#180809PurposeExpression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y257F)-TwinStrepDepositorInsertBTLAint (AA190-289, Y226F, Y243F, Y257F)-TwinStrep (BTLA Human)
ExpressionBacterialMutationBTLA (Y226F, Y243F, Y257F)PromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-2-GST-PD-1icd (AA194-288, Y223F, Y248F)
Plasmid#180815PurposeExpression of GST fused human PD-1 intracellular domain (AA194-288, Y223F, Y248F)DepositorInsertPD-1int (AA194-288, Y223F, Y248F) (PDCD1 Human)
ExpressionBacterialMutationPD-1 (Y223F, Y248F)PromoterTacAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMH011_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry
Plasmid#179434PurposeDual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertTRE-cit-HS4-mch (SH)
ExpressionMammalianPromoterpEF alpha, pRSVAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL006_phiC31_neo-5xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry
Plasmid#179425PurposeDual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integrationDepositorInsertTRE-cit-(nospacer)-mch
ExpressionMammalianPromoterpEF alpha, pRSVAvailable SinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-kappa-myc-dK-2xG4S-TMst
Plasmid#145766PurposeExpresses myc-dL5(E52K, L91S) low affinity Fluorogen Activating Protein (FAP) on the extracellular surface anchored to the mammalian cell plasma membrane with the PDGFR transmembrane domain.DepositorInsertkappa-myc-dK-2xG4S-TMst
ExpressionMammalianMutationThe dK FAP is dL5 with mutations at E50K, L89S, a…PromoterCMVAvailable SinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRalpha-Y206S
Plasmid#136435PurposeMammalian expression plasmid for c-myc-tagged PDGFRA (Y206S mutant)DepositorInsertPDGFRalpha (PDGFRA Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationY206S (disrupts PDGF binding, but still binds HCM…PromoterCMVAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSlick-Neo-KANK1
Plasmid#121983PurposeTetracycline-inducible expression of untagged full-length KANK1DepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCri System
Plasmid Kit#1000000058PurposePlasmids used for cytoplasmic and periplasmic/extracellular heterologous protein expression in E. coli, Bacillus subtilis, and Pichia pastoris.DepositorApplicationGene Expression and LabelingVector TypeBacterial Expression, Yeast ExpressionAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
ScEnSor Kit
Plasmid Kit#1000000215PurposeTo investigate the intracellular environment of Saccharomyces cerevisiae with a variety of biosensors.DepositorApplicationGene Expression and LabelingVector TypeYeast ExpressionAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS
Plasmid#115694PurposeAll-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.DepositorInsertsNmeCas9
Nme-sgRNA
UseCRISPRTagsNLS and NLS, HAExpressionInsect and MammalianMutationNone and fusion of repeat/tracrRNA to make a sgRNAPromoterEF-1 alpha and U6Available SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SIGLEC7-COMP5AP-AviTag-9xHis
Plasmid#157337PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2(WT)-Fc(IgG1)
Plasmid#145163PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgG1)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-NOTCH4(Trunc1)-Fc(DAPA)-AviTag-6xHis
Plasmid#157040PurposeMammalian expression of cell-surface protein partial extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertNOTCH4 (NOTCH4 Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only