We narrowed to 15,156 results for: pET

1. pETSUMO2_Bacteroidetes-bGSDM

   Plasmid

   #212124

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Bacteroidetes bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETSUMO2_Runella-bGSDM

   Plasmid

   #212123

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by Runella zeae. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Runella bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETSUMO2_Vitiosangium-bGSDM

   Plasmid

   #212127

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Vitiosangium species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Vitiosangium bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETSUMO2_Gillisia-bGSDM

   Plasmid

   #212128

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Gillisia species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Gillisia bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETSUMO2_Bradyrhizobium-bGSDM

   Plasmid

   #212126

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Bradyrhizobium species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Bradyrhizobium bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET47b-T7-dAsCas12f

   Plasmid

   #204641

   Purpose

   Expression of an inactive AsCas12f in E. coli

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   inactive AsCas12f
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D225A

   Promoter

   T7

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJL1-CyPet

   Plasmid

   #102636

   Purpose

   In vitro expression of CyPet from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   CyPet
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET23a-LIR-1 D1-D2

   Plasmid

   #12103

   Depositor

   Pamela Bjorkman

   Article

   Willcox et al Nat Immunol. 2003 Sep . 4(9):913-9.

   Insert

   Leukocyte immunoglobulin-like receptor 1 (LILRB1 Human)
   

   Expression

   Bacterial

   Mutation

   Amino acids 1−198*

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

9. petRNA-FANCF-v3'

   Plasmid

   #181803

   Purpose

   petRNA FANCF v3'

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

   Insert

   ribozyme-petRNA
   

   Use

   CRISPR

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Grp1-PH pET11a

    Plasmid

    #71377

    Purpose

    Phosphoinositide Binding

    Depositor

    Mark Lemmon

    Article

    Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

    Insert

    Grp1-PH (Cyth3 Mouse)
    

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 264-391

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pET28-Kss1

    Plasmid

    #52682

    Purpose

    Expression of Kss1 in bacteria

    Depositor

    Benjamin Turk

    Article

    Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

    Insert

    KSS1 (KSS1 Budding Yeast)
    

    Tags

    His6 and T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pET28a(+)-SUMO-A'-l4-G'

    Plasmid

    #209124

    Purpose

    Bacterial expression of CC-GEMS ligand SUMO-A'-l4-G'

    Depositor

    Tom de Greef

    Article

    Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

    Insert

    SUMO-A'-l4-G'
    

    Expression

    Bacterial

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETCON-TNF

    Plasmid

    #67748

    Purpose

    Tumor Necrosis Factor Alpha Yeast Display Plasmid

    Depositor

    Timothy Whitehead

    Article

    Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

    Insert

    TNF-Alpha
    

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM41-EcPPK

    Plasmid

    #38334

    Depositor

    Florian Freimoser

    Insert

    E. coli polyphosphate kinase (ppk Escherichia coli)
    

    Tags

    6xHIS, MBP, and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pET28a-Tyr-P(11)4

    Plasmid

    #185784

    Purpose

    Encodes enzyme tyrosinase linked to P(11)4 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    Tyr-P(11)4
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET24a MDH_STRAW

    Plasmid

    #204240

    Purpose

    Bacterial expression of S. avermitilis malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDH_STRAW (mdh Streptomyces avermitilis)
    

    Tags

    6X His

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFS_0353_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2

    Plasmid

    #116965

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21c-IF3mt

    Plasmid

    #31078

    Depositor

    Linda Spremulli

    Article

    Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

    Insert

    Mature form human mitochondrial translational initiation factor 3 (MTIF3 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Includes coding sequence for the mature form. Ami…

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

19. M26I DJ-1 pET15b

    Plasmid

    #60677

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

    Insert

    M26I DJ-1 (PARK7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26I

    Promoter

    T7

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pET15-EF4n_mFAP2b

    Plasmid

    #159464

    Purpose

    Encodes for EF4n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    EF4n_mFAP2b
    

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET15-EF1p3_mFAP2b

    Plasmid

    #159470

    Purpose

    Encodes for EF1p3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    EF1p3_mFAP2b
    

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET15-mFAP10

    Plasmid

    #159463

    Purpose

    Encodes for mFAP10 with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    mFAP10
    

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET19b Adrm1

    Plasmid

    #19423

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

    Insert

    adhesion regulating molecule 1 (ADRM1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pET28a-Vx3(A7)

    Plasmid

    #35526

    Depositor

    Robert Cohen

    Article

    Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

    Insert

    Vx3(A7)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Changed G2 to P

    Promoter

    T7

    Available Since

    April 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET28(+)-NudC

    Plasmid

    #175022

    Purpose

    Purification of NudC enzyme

    Depositor

    Jeppe Vinther

    Article

    Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

    Insert

    nudC
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28a-FAM86A

    Plasmid

    #85117

    Purpose

    bacterial expression of FAM86A

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EEF2KMT (FAM86A Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET15-MTFmt

    Plasmid

    #31174

    Depositor

    Linda Spremulli

    Article

    Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

    Insert

    Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)
    

    Tags

    His and thrombin cleavage

    Expression

    Bacterial

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

28. pET28a-MSP1D1deltaH4/2

    Plasmid

    #71712

    Purpose

    Membrane scaffold protein (MSP), half of helix 4 truncated

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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29. pEThT-RvCAHS12

    Plasmid

    #192468

    Purpose

    Bacterial expression of RvCAHS12

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS12
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET22-HT/prsA

    Plasmid

    #73624

    Purpose

    Produces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag

    Depositor

    James Williamson, T. J. Kappock

    Article

    Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

    Insert

    ribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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