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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,055 results for: Coli

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Showing: 6581 - 6600 of 14055 results

  5. Tn7 integration plasmid

    Plasmid
    #154134
    Purpose
    Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.
    Depositor
    Shimon Bershtein
    Article
    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038
    Inserts
    left and right end of Tn7 arm
    Spectinomycin resistant gene
    Expression
    Bacterial
    Promoter
    Spectinomycin resistance gene promoter and no
    Available Since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154134-plasmid-map-sequence-id-306811

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  7. pCasTet-λ

    Plasmid
    #128318
    Purpose
    Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.
    Depositor
    Christopher Johnston
    Article
    Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p
    Insert
    TetR and pTetR/TetO
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    Constitutive expression of cas9 and anhydro-tetra…
    Promoter
    tet promoter
    Available Since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128318-plasmid-map-sequence-id-254797

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  8. pSAM_AraC

    Plasmid
    #91569
    Purpose
    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.
    Depositor
    Harry Mobley
    Article
    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.
    Insert
    AraC/PBAD
    Expression
    Bacterial
    Available Since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91569-plasmid-map-sequence-id-181631

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  10. pMS_366

    Plasmid
    #182131
    Purpose
    expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation
    Depositor
    George Church
    Article
    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118
    Inserts
    retron RNA
    ec.86 RT
    CspRecT
    Mutation
    incorporated rifampicin-resistance-conferring don…
    Available Since
    Sept. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    182131-plasmid-map-sequence-id-355782

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  13. Keasling-2337

    Plasmid
    #100168
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    lacIq-Ptrc-ADS
    Expression
    Bacterial
    Promoter
    trc
    Available Since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100168-plasmid-map-sequence-id-193496

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  17. pEM-Cas9HF1

    Plasmid
    #89961
    Purpose
    Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.
    Depositor
    Michael Lynch
    Article
    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021
    Insert
    Cas9HF1
    Expression
    Bacterial
    Mutation
    N497A/R661A/Q695A/Q926A
    Promoter
    pTet
    Available Since
    June 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    89961-plasmid-map-sequence-id-181339

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Showing: 6581 - 6600 of 14055 results