We narrowed to 15,824 results for: LEA

1. GFP-Drosha218-1374

   Plasmid

   #62523

   Purpose

   N-terminal aa1-217 of Drosha is deleted

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   human Drosha with aa 1-217 deleted (DROSHA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-217

   Promoter

   Available since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

2. GFP-Drosha270-1374

   Plasmid

   #62524

   Purpose

   N-terminal aa1-269 of Drosha is deleted

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   human Drosha with aa 1-269 deleted (DROSHA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-269

   Promoter

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pFLAG-74.84S Tetherin

   Plasmid

   #41071

   Depositor

   Paul Spearman

   Article

   Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

   Insert

   Tetherin (BST2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   74S, 84S

   Promoter

   CMV

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pEF Doc2beta 6X mutant GFP

   Plasmid

   #128823

   Purpose

   To visualize Doc2beta 6X mutant in PC12 cells

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2beta (Doc2b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D163A, D218A, D220N, D303A, D357A, D359A

   Promoter

   EF-1alpha

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 3695 pGEX4T-3 MLL AD mut.E

   Plasmid

   #8867

   Depositor

   Stanley Korsmeyer

   Article

   Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

   Insert

   MLL min A.D. (KMT2A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   ILPSDIMDFVL --> ILPADIMDFVL

   Promoter

   Available since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

6. HOXD4 (murine) HIS-tag pET

   Plasmid

   #8548

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXD4 (Hoxd4 Mouse)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   makes protein by Western and by in vitro transcri…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pMGF157

   Plasmid

   #96972

   Purpose

   ish1-mCherry insertion cassette with Hygro resistance gene

   Depositor

   Wesley Sundquist, Adam Frost

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   ish1 (ish1 Fission Yeast)
   

   Use

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   ish1 C-terminal domain fused with mCherry + hygro…

   Promoter

   N/A

   Available since

   Aug. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NG1301 pCI-TPI-4MS2-SMG5(short)-4H

   Plasmid

   #65806

   Purpose

   NMD reporter mRNA

   Depositor

   Niels Gehring

   Article

   Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

   Insert

   TPI (TPI1 Human)
   

   Use

   Tags

   4 MS2 stem loops, SMG5 3'UTR shortened versi…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV-EF1a-V5-Mfsd10

   Plasmid

   #120238

   Purpose

   Expresses V5-tagged Mfsd10 in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Mfsd10 (Mfsd10 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBABEhygro-Flag-ZO-2-delta-N

    Plasmid

    #27476

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of coding nucleotides from 1-351. This r…

    Promoter

    Available since

    March 31, 2011

    Availability

    Academic Institutions and Nonprofits only

11. GFP-DroshaS300A

    Plasmid

    #62526

    Purpose

    Serine 300 of Drosha is mutated to alanine

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

    Insert

    human Drosha with serine 300 changed to alanine (DROSHA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed serine 300 to alanine

    Promoter

    Available since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

12. GFP-DroshaS302A

    Plasmid

    #62527

    Purpose

    Serine 302 of Drosha is mutated to alanine

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

    Insert

    human Drosha with serine 302 changed to alanine (DROSHA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed serine 302 to alanine

    Promoter

    Available since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

13. PD-1-miSFIT-1-A, 5-A

    Plasmid

    #124682

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-1 (PDCD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCin4-3xFLAG-MKL1 L272A

    Plasmid

    #19850

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 L272A (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Leucine 272 to Alanine

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

15. NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75233

    Purpose

    CRISPR/Cas9 plasmid against human NFATc2

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc2 (NFATC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCin4-3xFLAG-MKL1 D444-630

    Plasmid

    #19840

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 D444-630 (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 444-630

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5 FRT TO myc Separase delta 55/S1126A

    Plasmid

    #59825

    Purpose

    Allows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    delta 55/S1126A

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCin4-3xFLAG-MKL1 S449A

    Plasmid

    #19842

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 S449A (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Serine 449 to Alanine

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

19. HOXB9 (murine) HIS-tag pET

    Plasmid

    #8541

    Depositor

    Corey Largman

    Article

    Chang et al Mol Cell Biol 1996 Apr;16(4):1734-45.

    Insert

    HOXB9 (Hoxb9 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Missing the first 13 amino acids of HOXB9; makes …

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pBacPak-HA-Non-stop

    Plasmid

    #52289

    Purpose

    Expresses Drosophila Non-stop

    Depositor

    Jerry Workman

    Article

    Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

    Insert

    Non-stop (not Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only