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Addgene

We narrowed to 15,824 results for: LEA

Showing: 6601 - 6620 of 15824 results
  1. pBiex PKC - Peptide 14 mCit mCer

    Plasmid
    #83565
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.
    Insert
    PKC alpha (PRKCA Human)
    Use
    Tags
    10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. pCVL.SSA TLR (Ani)

    Plasmid
    #45577
    Purpose
    Codes for the SSA-TLR with the target site for I-Ani I in a lentiviral backbone
    Depositor
    Inserts
    5' iRFP arm
    eGFP with I-Ani I TS
    +3 mCherry
    3' iRFP arm
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    embedded I-Ani I TS from 163-185bp, truncated 25 …
    Promoter
    SFFV
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
  3. pNF-KB-Split PS Intein-C tTA

    Plasmid
    #242035
    Purpose
    C_terminal segment of a blue_light_controlled split PS_intein tTA gene_expression system, co_expressing mCherry under an NF_KB_inducible promoter
    Insert
    Split PS Intein-C tTA
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    The CMV promoter in the original vector was repla…
    Promoter
    NF-KB
    Available since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pET-28a_VIM-2

    Plasmid
    #242840
    Purpose
    Expresses His6-TEV-VIM-2 in bacteria, comprising a His6-tag, the Tobacco etch virus (TEV) cleavage site “ENLYFQ/G", and the VIM-2 sequence (UniPROT Q5U7L7)
    Depositor
    Insert
    Metallo-beta-lactamase VIM-2
    Use
    Tags
    His6 + Tobacco etch virus (TEV) cleavage site “EN…
    Expression
    Bacterial
    Mutation
    deleted residues 1-26
    Promoter
    T7
    Available since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pET-28a_VIM-20

    Plasmid
    #242842
    Purpose
    Expresses His6-TEV-VIM-20 in bacteria, comprising a His6-tag, the Tobacco etch virus (TEV) cleavage site “ENLYFQ/G", and the VIM-20 sequence (UniPROT A0A344X7M2)
    Depositor
    Insert
    Metallo-beta-lactamase VIM-20
    Use
    Tags
    His6 + Tobacco etch virus (TEV) cleavage site “EN…
    Expression
    Bacterial
    Mutation
    deleted residues 1-26
    Promoter
    T7
    Available since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pET-28a_VIM-15

    Plasmid
    #242841
    Purpose
    Expresses His6-TEV-VIM-15 in bacteria, comprising a His6-tag, the Tobacco etch virus (TEV) cleavage site “ENLYFQ/G", and the VIM-15 sequence (UniPROT B4YAJ6)
    Depositor
    Insert
    Metallo-beta-lactamase VIM-15
    Use
    Tags
    His6 + Tobacco etch virus (TEV) cleavage site “EN…
    Expression
    Bacterial
    Mutation
    deleted residues 1-26
    Promoter
    T7
    Available since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pET-28a_VIM-31

    Plasmid
    #242843
    Purpose
    Expresses His6-TEV-VIM-31 in bacteria, comprising a His6-tag, the Tobacco etch virus (TEV) cleavage site “ENLYFQ/G", and the VIM-31 sequence (UniPROT I3RJZ3)
    Depositor
    Insert
    Metallo-beta-lactamase VIM-31
    Use
    Tags
    His6 + Tobacco etch virus (TEV) cleavage site “EN…
    Expression
    Bacterial
    Mutation
    deleted residues 1-26
    Promoter
    T7
    Available since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. enDR3-TEV-mEGFP-NLS (pRS1451)

    Plasmid
    #240546
    Purpose
    Expression of the engineered N-terminal hybrid-binding domain of RNase H3 (enDR3) for capturing R-loops. A TEV cleavage site, mEGFP and nuclear localization signal (NLS) are included in the fusion.
    Depositor
    Insert
    enDR3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L52M
    Promoter
    Available since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. pEJS2783 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-16 CMV-BFP

    Plasmid
    #240513
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Insert
    AcrIIC3
    Use
    Tags
    FLAG, hsa-miR-16 Target Sites, and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. pEJS2784 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-20a CMV-BFP

    Plasmid
    #240514
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Insert
    AcrIIC3
    Use
    Tags
    FLAG, hsa-miR-20 Target Sites, and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. pEJS2785 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-125a CMV-BFP

    Plasmid
    #240515
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Insert
    AcrIIC3
    Use
    Tags
    FLAG, hsa-miR-125a Target Sites, and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  12. pEJS2786 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-151a CMV-BFP

    Plasmid
    #240516
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Insert
    AcrIIC3
    Use
    Tags
    FLAG, hsa-miR-151a Target Sites, and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 6601 - 6620 of 15824 results