We narrowed to 14,067 results for: LEA
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Plasmid#177902PurposeBacterial expression of his10-tagged human TIGIT Intracellular DomainDepositorInsertTIGIT (aa 163-244 only) (TIGIT Human)
Tags10xHis-Thrombin cut site-T7 tagExpressionBacterialPromoterT7Available sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_CRFmut
Plasmid#208659PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in neuronsDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
UseAAVPromoterhSynAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-Sniper SpCas9-NLS-6xHis
Plasmid#207374PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertSniper SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationF539S, M763I, K890NPromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cas12i-Max-T2A-EGFP
Plasmid#188268PurposeMammalian expression, Genome editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas12i-Max
UseCRISPRExpressionMammalianMutationnonePromoterCAGAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cas12i-HiFi-T2A-EGFP
Plasmid#188269PurposeMammalian expression, Genome editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas12i-HiFi
UseCRISPRExpressionMammalianMutationnonePromoterCAGAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-Hook3
Plasmid#198525PurposeExpression of GAL4 DNA-binding domain (BD)-Hook3 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertHook3 (HOOK3 Human)
TagsGAL4-DNA binding domain (BD) fragmentExpressionYeastPromoterADH1Available sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD-SKW-HHL
Plasmid#190632PurposeBacterial expression of genetically encoded potassium indicator SKW-HHLDepositorInsertSKW-HHL
ExpressionBacterialAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
T10-T7-hMOV10
Plasmid#185052Purposeexpression of WT human MOV10DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcDNA WT hMOV10 (MOV10 Human)
TagsT7ExpressionMammalianAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RBP-LOV2
Plasmid#185792PurposeEncodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linkerDepositorInsertRBP-LOV2
Tags10xHis, TEV protease cleavage site after His TagExpressionBacterialPromoterT7Available sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGPN-ACE2
Plasmid#187483PurposeExpression vector - ACE2DepositorAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-CeNLuc_SV40:PuroR
Plasmid#183036PurposeExpresses the FLAG-tagged CeNLuc (Blue LumiFluor) in mammalian cells.DepositorInsertCeNLuc LumiFluor
UseLentiviralTagsFLAGExpressionMammalianPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUb LwaCas13a + LwaCas13a Guide RNA
Plasmid#176307PurposeExpresses LwaCas13a and its associated guide RNA in Drosophila cellsDepositorInsertLwaCas13a
TagsHA tagExpressionInsectMutationE656G, I1060V, D1076G- please see depositor comme…PromoterUbi-p63eAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_NWS.HA.V5_mCherry-NLS
Plasmid#178283PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsNWS.HA.V5 and Nuclear Localization SignalPromotereF1aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_C.HA.V5_mCherry-NLS
Plasmid#178242PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsC.HA.V5 and Nuclear Localization SignalPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PB1-AG-FRB
Plasmid#178862PurposeMammalian expression of PB1-AzamiGreen-FRBDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPB1-AzamiGreen-FRB
ExpressionMammalianPromoterCMVAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FKBP-mTagBFP2-MCS
Plasmid#178864PurposeMammalian expression of a protein of interest fused to the C-terminus of FKBP-mTagBFP2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFKBP-mTagBFP2
ExpressionMammalianPromoterCMVAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TUBG1
Plasmid#171967PurposepGEX2T-TUBG1 bacterial expression plasmid for production of recombinant GST fusion proteinDepositorInsertTUBG1 (TUBG1 Human)
TagsGSTExpressionBacterialMutationR275H (Please see depositor comments)Available sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pISH-NQO1
Plasmid#136766PurposeTemplate to synthesize riboprobes for in situ hybridization (ISH) for Nqo1DepositorInsertNqo1 (Nqo1 Mouse)
UseIn situ probeMutationISH Probe insert, V184A- Please see depositor co…Available sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c021
Plasmid#139758PurposeProtein expression/crystallography (PDB:5QT0)DepositorInsertTBXT, DNA-binding domain, G177D (TBXT Human)
TagsHis6-SUMOExpressionBacterialMutationG177D; contains only amino acids E41-N211PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-24a-STE2
Plasmid#133430PurposeYeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.DepositorInsertSTE2 (STE2 Budding Yeast)
ExpressionBacterialMutationBoth native Cys (C59S/C252A) have been mutated. S…Available sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only