We narrowed to 17,414 results for: LEA

1. pAAV-CaMKII-STChRger2-TS-EYFP

   Plasmid

   #129393

   Purpose

   Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   soma targeted ChRger2
   

   Use

   AAV

   Tags

   Kv2.1-TS-EYFP

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEZT-GST-SLFN2

   Plasmid

   #174319

   Purpose

   Expression of recombinant GST-SLFN2

   Depositor

   Bruce Beutler

   Article

   Yue et al Science. 2021 May 14;372(6543). pii: 372

   Insert

   GST-SLFN2 (Slfn2 Mouse)
   

   Use

   Bacmam, baculovirus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJP-HBB-nLuc

   Plasmid

   #175431

   Purpose

   Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nLUC plus 5' HBB leader
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ad-Flag-XBP1u

   Plasmid

   #63677

   Purpose

   Adenoviral vector for XBP1u expression

   Depositor

   Lingfang Zeng, Qingbo Xu

   Article

   Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

   Insert

   XBP1 (XBP1 Human)
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   unspliced

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

5. pCI syn iGluu

   Plasmid

   #106122

   Purpose

   Fast indicator for synaptic glutamate imaging (synapsin promoter)

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR S72T variant
   

   Tags

   Myc and PDGFR

   Expression

   Mammalian

   Mutation

   Changed Ser 72 to Thr

   Promoter

   synapsin

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EFS-DIO-GRAB_Ado1.0med

   Plasmid

   #153292

   Purpose

   Express the medium-affinity adenosine sensor GRAB_Ado1.0med in Cre positive cells

   Depositor

   Yulong Li

   Article

   Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Adenosine sensor GRAB_Ado1.0med (medium affinity)
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNOC-CRISPR-P2A-BlastR

   Plasmid

   #98147

   Purpose

   Expresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   P2A-BlastR fusion with Cas9-Nlux

   Promoter

   Ribi promoter

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Sniper-Cas9

   Plasmid

   #138559

   Purpose

   Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   Sniper-Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   F539S, M763I, K890N

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AM77_pU6.Sa-gRNA.CLYBL

   Plasmid

   #199237

   Purpose

   Expression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   S. aureus gRNA spacer
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET26b-SET

    Plasmid

    #8822

    Depositor

    Judy Lieberman

    Article

    Beresford et al J Biol Chem 2001 Nov 16;276(46):43285-93

    Insert

    SET (SET Human)
    

    Tags

    6XHis

    Expression

    Bacterial

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pcDNAintron-CHIKV-nsp3-c3xFLAG

    Plasmid

    #215696

    Purpose

    Produces the Chikungunya virus nonstructural protein 3 with a c-terminal 3xFLAG tag

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV nsp3
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pRevTRE MKL1-N100

    Plasmid

    #19848

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1-N100 (MRTFA Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Mutation

    deleted amino acids 1-100

    Available Since

    Jan. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pRSFDuet-1_VNp15-StefinA

    Plasmid

    #182405

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-stefinA fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-stefin (CSTA Human)
    

    Tags

    Vesicle Nucleating peptide 15 (VNp15)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-REPORT(PGK/CMV)-GtwyA

    Plasmid

    #208574

    Purpose

    LV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    mnucTomato
    HSVtk
    Neomycin
    d2nucEGFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    PGK/CMV and minP

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CamKIIα-CRY2PHR-P2A-CIBN-mCherry-VAMP2

    Plasmid

    #178574

    Purpose

    Lentiviral vector for CamKIIα::Opto-vTrap

    Depositor

    Won Do Heo

    Article

    Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

    Insert

    CRY2PHR-P2A-CIBN-mCherry-VAMP2
    

    Use

    Lentiviral

    Promoter

    CaMKIIa

    Available Since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Diacylglycerol Kinase Zeta

    Plasmid

    #85454

    Purpose

    expression of Diacylglycerol Kinase Zeta

    Depositor

    Stephen Gee

    Article

    Hogan et al J Biol Chem. 2001 Jul 13;276(28):26526-3

    Insert

    Diacylglycerol Kinase Zeta (DGKZ Human)
    

    Tags

    3x HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHEN2 F8-2

    Plasmid

    #186033

    Purpose

    Express VHH F8-2 with 3 c-myc tag in E. coli periplasm

    Depositor

    Isabelle Landrieu

    Article

    Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

    Insert

    VHH F8-2
    

    Tags

    6His and Myc

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTetOne-Six1-myc

    Plasmid

    #138530

    Purpose

    Inducible expression of Six1 with a myc tag

    Depositor

    Christopher Grigsby, Ana Teixeira

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    Six1 (Six1 Mouse)
    

    Tags

    3x myc tag

    Expression

    Mammalian

    Promoter

    TRE3Gs

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry Codon 59 sgRNA

    Plasmid

    #109431

    Purpose

    MLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporter

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    mCherry codon 59 gRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-p97-E578Q

    Plasmid

    #85672

    Purpose

    Expression of human p97 E578Q mutant with N-terminal GFP tag

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    p97 (Vcp Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    E578Q

    Promoter

    CMV

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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