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pDONR223_DARC_WT_V5

Plasmid

#82973

Purpose

Gateway Donor vector containing DARC, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ACKR1 (ACKR1 Human)

Use

Gateway entry vector

Mutation

F326S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DARC_p.W253R

Plasmid

#82749

Purpose

Gateway Donor vector containing DARC, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ACKR1 (ACKR1 Human)

Use

Gateway entry vector

Mutation

W253R; S326F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DARC_p.T211S

Plasmid

#82750

Purpose

Gateway Donor vector containing DARC, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ACKR1 (ACKR1 Human)

Use

Gateway entry vector

Mutation

T211S; S326F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-SaKKH-spA-miniU6-miniSdd6-MmHPD-T2

Plasmid

#204852

Purpose

AAV vector for cytosine base editing using miniSdd6 at HPD-T2 target site in mouse

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd6-nSaCas9KKH(D10A)-UGI

Use

AAV and CRISPR

Mutation

D10A, E782K, N968K, R1015H in SaCas9

Promoter

EFS, miniU6

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_C1orf150_WT

Plasmid

#81842

Purpose

Gateway Donor vector containing C1ORF150 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

C1ORF150 (GCSAML Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_C1orf150_p.Y89N

Plasmid

#81304

Purpose

Gateway Donor vector containing C1ORF150 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

C1ORF150 (GCSAML Human)

Use

Gateway entry vector

Mutation

Y89N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_C1orf150_p.G2E

Plasmid

#81309

Purpose

Gateway Donor vector containing C1ORF150 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

C1ORF150 (GCSAML Human)

Use

Gateway entry vector

Mutation

G2E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-C-6His-mEsrra

Plasmid

#173152

Purpose

mouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cells

Depositor

Brijesh Kumar Singh

Article

Tripathi et al bioRxiv 2021.11.16.468891

Insert

ORF clone Estrogen-related receptor alpha (Esrra) (Esrra Mouse)

Tags

His

Expression

Mammalian

Mutation

Wild type

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA-Cre-GpNLuc-sgmSTING

Plasmid

#208386

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine STING gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgSTING (Sting1 Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1784 - pAAV SYN1 hChR2(H134R)-V5-ER-LBD (ChRERa)

Plasmid

#201820

Purpose

An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET imaging

Depositor

Christopher Richie

Article

Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

Insert

hChR2(H134R)-V5-ER-LBD (ChRERa)

Use

AAV

Promoter

SYN1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC1822 - pAAV SYN1 DIO hChR2(H134R)-V5-ER-LBD (ChRERa)

Plasmid

#201821

Purpose

An adeno-associated viral vector expressing Cre-dependent channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET

Depositor

Christopher Richie

Article

Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

Insert

hChR2(H134R)-V5-ER-LBD (ChRERa)

Use

AAV

Promoter

SYN1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC1845 - pAAV CaMKII hChR2(H134R)-V5-ER-LBD (ChRERa)

Plasmid

#201822

Purpose

An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CaMKii promoter, for use in PET imaging

Depositor

Christopher Richie

Article

Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

Insert

hChR2(H134R)-V5-ER-LBD (ChRERa)

Use

AAV

Promoter

CaMKii

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV_IRES_GFP_CD16a

Plasmid

#196189

Purpose

Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

Depositor

Ana Ruiz-Saenz

Article

Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

Insert

CD16a (FCGR3A Human)

Use

Retroviral

Expression

Mammalian

Mutation

changed Phenylalanine 158 to Valine

Promoter

LTR

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Gad65-WPRE-HGHpA

Plasmid

#184631

Purpose

Expresses Gad65, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gad65 (Gad2 Mouse)

Use

AAV

Promoter

EF1a

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSLCAR-CD19-28z

Plasmid

#135991

Purpose

Modular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Scott McComb

Article

Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

Insert

FMC63-28-3z-P2A-EGFP (CD28, CD247 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a-short

Available Since

June 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSLCAR-CD19-CD3z

Plasmid

#135993

Purpose

Modular CD3z alone CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Scott McComb

Article

Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

Insert

FMC63-3z-P2A-EGFP (CD247, CD28 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a-short

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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MOS

Plasmid

#64120

Purpose

A modified episomal (EBNA1/oriP) vector expressing human OCT4 and SOX2 genes

Depositor

Linzhao Cheng

Article

Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

Insert

OCT4-2A-SOX2 (POU5F1, SOX2 Human)

Tags

HA and Myc

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pEP4 E02S EN2K

Plasmid

#20925

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Klf4

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; Nanog and Klf4 (SOX2, POU5F1, NANOG, KLF4 Human)

Use

Episomal/ebna

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pEP4 E02S EN2L

Plasmid

#20922

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Lin28

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; Nanog and Lin28 (POU5F1, SOX2, NANOG, LIN28A Human)

Use

Episomal/ebna

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pX458-ECFP

Plasmid

#112220

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

Depositor

Yinming Liang

Article

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

ECFP

Use

CRISPR

Expression

Mammalian

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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