We narrowed to 59,446 results for: cloning

1. pCA_o-2

   Plasmid

   #202092

   Purpose

   Receiver cloning vector for odd-2 level constructs. Contains lacZ for blue/white screening.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pSLIK_hygro_BirA

   Plasmid

   #136537

   Purpose

   Lentiviral expression vector for an inducible BirA

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   BirA
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Expression

   Mammalian

   Available Since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-PB2-B/BRISBANE/60/2008

   Plasmid

   #187917

   Purpose

   Expresses PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase basic protein 2
   

   Tags

   UNTAGGED

   Promoter

   CMV Promoter

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. P2A-NLS-tdtomato-EPG backbone

   Plasmid

   #215548

   Purpose

   An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRL1342

   Plasmid

   #70691

   Purpose

   RSF1010-based vector with extensive polylinker, and resistance to Cm and Em. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.

   Depositor

   Peter Wolk

   Article

   Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

   Type

   Empty backbone

   Use

   Bacterial shuttle vector

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. DL015:CMV::iPAK4

   Plasmid

   #177880

   Purpose

   ibox-PAK4cat (iPAK4) fusion with CMV promoter, cloned into a lentivirus backbone

   Depositor

   Adam Cohen

   Article

   Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   iPAK4
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. P2A-mCherry-EhT backbone

   Plasmid

   #215549

   Purpose

   An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomato

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. DL016:CMV::eGFP-iPAK4

   Plasmid

   #177881

   Purpose

   eGFP-IPAK4 fusion with CMV promoter, cloned into a lentivirus backbone

   Depositor

   Adam Cohen

   Article

   Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   iPAK4
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-HA-hTAF4

   Plasmid

   #17983

   Depositor

   Naoko Tanese

   Article

   Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

   Insert

   TAF4 (TAF4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   missing 5' end

   Available Since

   Aug. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

10. puc19-pCas13a

    Plasmid

    #118963

    Purpose

    Intermediate/cloning vector that can be used for sub-cloning into final destination vectors based on one's requirements.

    Depositor

    Magdy Mahfouz

    Article

    Aman et al Genome Biol. 2018 Jan 4;19(1):1. doi: 10

    Insert

    Cas13a
    

    Expression

    Plant

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCPP5052

    Plasmid

    #128729

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopAM1
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-15b PAI-RBP

    Plasmid

    #45390

    Depositor

    Thomas Gelehrter

    Article

    Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

    Insert

    PAI-RBP (SERBP1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pDONR221-kcnk18

    Plasmid

    #209000

    Purpose

    Riboprobe generation

    Depositor

    GuangJun Zhang

    Article

    Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

    Insert

    kcnk18 (kcnk18 Zebrafish)
    

    Use

    Cloning

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGuide

    Plasmid

    #64711

    Purpose

    Cloning and expression of Streptococcus pyogenes guide RNA

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

    Insert

    Streptococcus pyogenes guide RNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBT13-splitD

    Plasmid

    #122597

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQTEV-TAGLN2

    Plasmid

    #31569

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    transgelin 2 (TAGLN2 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEN_TTmcs

    Plasmid

    #25755

    Purpose

    Entry vector with TRE-tight promoter followed by multiple cloning site.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Entry vector

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT137-splitC

    Plasmid

    #122605

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221_SLC22A2

    Plasmid

    #131964

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

    Insert

    SLC22A2 (SLC22A2 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pMG343

    Plasmid

    #176183

    Purpose

    preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    TPK2+ccdB dropout cassette
    

    Expression

    Yeast

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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